The Association of SERPINE2 Gene with COPD in a Chinese Han Population
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Purpose: Polymorphisms of several candidate genes have been studied and associated with the development of chronic obstructive pulmonary disease (COPD). One such candidate is the SERPINE2 (Serpin peptidase inhibitor, clade E member 2) gene. Materials and Methods: To assess whether the SERPINE2 gene is associated with COPD in a Chinese Han population. Samples were collected from a Chinese Han population and analyzed for the association of single nucleotide polymor phisms (SNPs) or haplotypes of SERPINE2 gene with COPD in a case-control study. Three SNPs including rs840088 G/A in intron 1, rs1438831 A/G in 5’ upstream sequence and rs3795879 G/A in intron 3 were detected using the polymerase chain reaction (PCR)-based restriction fragment length polymorphism technique in 409 COPD subjects and 411 controls. Genotyping of the SREPINE2 polymorphisms at positions rs840088, rs1438831and rs3795879 was performed. Results: We found that none of the rs840088G/A, rs1438831G/A and rs3795879 G/A polymorphisms were associated with the disease. The p-values were 0.630, 0.208 and 0.398 respectively. Conclusion: Our data suggested that there was no significant association between SERPINE2 polymorphism and COPD susceptibility in the Chinese Han population.
Abstract
Purpose: Polymorphisms of several candidate genes have been studied and associated with the development of chronic obstructive pulmonary disease (COPD). One such candidate is the SERPINE2 (Serpin peptidase inhibitor, clade E member 2) gene. Materials and Methods: To assess whether the SERPINE2 gene is associated with COPD in a Chinese Han population. Samples were collected from a Chinese Han population and analyzed for the association of single nucleotide polymor phisms (SNPs) or haplotypes of SERPINE2 gene with COPD in a case-control study. Three SNPs including rs840088 G/A in intron 1, rs1438831 A/G in 5’ upstream sequence and rs3795879 G/A in intron 3 were detected using the polymerase chain reaction (PCR)-based restriction fragment length polymorphism technique in 409 COPD subjects and 411 controls. Genotyping of the SREPINE2 polymorphisms at positions rs840088, rs1438831and rs3795879 was performed. Results: We found that none of the rs840088G/A, rs1438831G/A and rs3795879 G/A polymorphisms were associated with the disease. The p-values were 0.630, 0.208 and 0.398 respectively. Conclusion: Our data suggested that there was no significant association between SERPINE2 polymorphism and COPD susceptibility in the Chinese Han population.
참고문헌 (24)
- Pauwels RA, "GOLD Scientific Committee. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease. NHLBI", WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary, 2001, pp.163, 1256~1276
- World Health Report, , World Health Organization, ~
- Lopez AD, "Chronic obstructive pulmonary disease: current burden and future projections", Eur Respir J, 27, pp.397~412 (2006)
- Lieberman J, "Alpha 1-antitrypsin Pi-types in 965 COPD patients", Chest, 89, pp.370~373 (1986)
- Celli BR, "Airway obstruction in never smokers: results from the Third National Health and Nutrition Examination Survey", Am J Med, 118, pp.1364~1372 (2005)
- Behrendt CE, "Mild and moderate-to-severe COPD in nonsmokers: distinct demographic profiles", Chest, 128, pp.1239~1244 (2005)
- Sandford AJ, "Chronic obstructive pulmonary disease. 1: susceptibility factors for COPD the genotype-environment interaction", Thorax, 57, pp.736~741 (2002)
- Sandford AJ, "Genetic risk factors for chronic obstructive pulmonary disease", Curr Opin Pulm Med, 8, pp.87~94 (2002)
- Molfino NA, "Current thinking on genetics of chronic obstructive pulmonary disease", Curr Opin Pulm Med, 13, pp.107~113 (2007)
- Eddahibi S, "Polymorphism of the serotonin transporter gene and pulmonary hypertension in chronic obstructive pulmonary disease", Circulation, 108, pp.1839~1844 (2003)
- Yildiz P, "Gene polymorphisms of endothelial nitric oxide synthase enzyme associated with pulmonary hypertension in patients with COPD", Respir Med, 97, pp.1282~1288 (2003)
- Castaldi PJ, "Genetic associations with hypoxemia and pulmonary arterial pressure in COPD", Chest, 135, pp.737~744 (2009)
- Demeo DL, "The SERPINE2 gene is associated with chronic obstructive pulmonary disease", Am J Hum Genet, 78, pp.253~264 (2006)
- Zhu G, "The SERPINE2 gene is associated with chronic obstructive pulmonary disease in two large populations", Am J Respir Crit Care Med, 176, pp.167~173 (2007)
- Chappell S, "The SERPINE2 gene and chronic obstructive pulmonary disease", Am J Hum Genet, 79, pp.184~186 (2006)
- "Standards for the diagnosis and care of patients with chronic obstructive pulmonary disease. American Thoracic Society", Am J Respir Crit Care Med, 152, pp.S77~S121 (1995)
- Miller SA, "A simple salting out procedure for extracting DNA from human nucleated cells", Nucleic Acids Res, 16, pp.1215~ (1988)
- Smith OO, "Genetic", environmental determinants of adult chronic obstructive pulmonary disease and possible links with childhood wheezing, 2001, pp.2, 178~183
- Carter RE, "The gene for the serpin thrombin inhibitor (PI7), protease nexin I, is located on human chromosome 2q33-q35 and on syntenic regions in the mouse and sheep genomes", Genomics, 27, pp.196~199 (1995)
- Rossignol P, "Protease nexin-1 inhibits plasminogen activation-induced apoptosis of adherent cells", J Biol Chem, 279, pp.10346~10356 (2004)
- Mbebi C, "Thrombin receptor induction by injury-related factors in human skeletal muscle cells", Exp Cell Res, 263, pp.77~87 (2001)
- Lohmueller KE, "Meta-analysis of genetic association studies supports a contribution of common variants to susceptibility to common disease", Nat Genet, 33, pp.177~182 (2003)
- Hersh CP, "Attempted replication of reported chronic obstructive pulmonary disease candidate gene associations", Am J Respir Cell Mol Biol, 33, pp.71~78 (2005)
- Silverman EK, "Case-control association studies for the genetics of complex respiratory diseases", Am J Respir Cell Mol Biol, 22, pp.645~648 (2000)
