A Simple, Reproducible, Inexpensive, Yet Old-Fashioned Method for Determining Phagocytic and Bactericidal Activities of Macrophages
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Macrophages (Mo|) play a pivotal role in the protection system by recognizing and eliminating invading pathogenic bacteria. Phagocytosis and the killing of invading bacteria are major effector functions of Mo|. Although the phagocytic and bactericidal activitiesof Mo| have been analyzed via several methods using a light microscope, a fluorescence microscope, or a fluorescence-activatedcell sorter, expensive materials and equipment are usually required, and the methods are rather complicated. Moreover, it is impossible to determine both the phagocytic and bactericidal activities of Mo| simultaneously using these methods. In this review,we describe a simple, reproducible, inexpensive, yet old-fashioned method (antibiotic protection assay) for determining the phagocytic and bactericidal activities of Mø.
Abstract
Macrophages (Mo|) play a pivotal role in the protection system by recognizing and eliminating invading pathogenic bacteria. Phagocytosis and the killing of invading bacteria are major effector functions of Mo|. Although the phagocytic and bactericidal activitiesof Mo| have been analyzed via several methods using a light microscope, a fluorescence microscope, or a fluorescence-activatedcell sorter, expensive materials and equipment are usually required, and the methods are rather complicated. Moreover, it is impossible to determine both the phagocytic and bactericidal activities of Mo| simultaneously using these methods. In this review,we describe a simple, reproducible, inexpensive, yet old-fashioned method (antibiotic protection assay) for determining the phagocytic and bactericidal activities of Mø.
참고문헌 (39)
- Doherty GJ, "Mechanisms of endocytosis", Annu Rev Biochem, 78, pp.857~902 (2009)
- Underhill DM, "Information processing during phagocytosis", Nat Rev Immunol, 12, pp.492~502 (2012)
- Garin J, "The phagosome proteome: insight into phagosome functions", J Cell Biol, 152, pp.165~180 (2001)
- Fang FC, "Antimicrobial reactive oxygen and nitrogen species:concepts and controversies", Nat Rev Microbiol, 2, pp.820~832 (2004)
- Ray K, "Life on the inside: the intracellular lifestyle of cytosolic bacteria", Nat Rev Microbiol, 7, pp.333~340 (2009)
- Buchmeier NA, "Intracellular survival of wild-type Salmonella typhimurium and macrophage-sensitive mutants in diverse populations of macrophages", Infect Immun, 57, pp.1~7 (1989)
- Skerrett SJ, "Recombinant murine interferon-gamma reversibly activates rat alveolar macrophages to kill Legionella pneumophila", J Infect Dis, 166, pp.1354~1361 (1992)
- Inoue S, "Intracellular killing of Listeria monocytogenes in the J774.1 macrophage-like cell line and the lipopolysaccharide (LPS)-resistant mutant LPS1916 cell line defective in the generation of reactive oxygen intermediates after LPS treatment", Infect Immun, 63, pp.1876~1886 (1995)
- Stevanin TM, "Nitric oxide detoxification systems enhance survival of Neisseria meningitidis in human macrophages and in nasopharyngeal mucosa", Infect Immun, 73, pp.3322~3329 (2005)
- Emoto M, "Alpha-galactosylceramide promotes killing of Listeria monocytogenes within the macrophage phagosome through in-variant NKT-cell activation", Infect Immun, 78, pp.2667~2676 (2010)
- Mandell GL, "Interaction of intraleukocytic bacteria and antibiotics", J Clin Invest, 52, pp.1673~1679 (1973)
- Lobo MC, "The effect of antibiotics on Escherichia coli ingested by macrophages", Proc Soc Exp Biol Med, 142, pp.1048~1050 (1973)
- Vaudaux P, "Gentamicin antibacterial activity in the presence of human polymorphonuclear leukocytes", Antimicrob Agents Chemother, 16, pp.743~749 (1979)
- Bryskier A, "Antimicrobial agents" (2005), ASM Press
- Vakulenko SB, "Versatility of aminoglycosides and prospects for their future", Clin Microbiol Rev, 16, pp.430~450 (2003)
- Klein JO, "Gentamicin: activity in vitro and observations in 26 patients", Am J Med Sci, 248, pp.528~544 (1964)
- Waitz JA, "Recent microbiological studies with gentamicin", Infect Dis, 119, pp.355~360 (1969)
- Espaze EP, "Antibiotic susceptibilities of Listeria: in vitro studies", Infection, 16(Suppl 2), pp.S160~S164 (1988)
- Ho YI, "In-vitro activities of aminoglycosideaminocyclitols against mycobacteria", J Antimicrob Chemother, 40, pp.27~32 (1997)
- Wilson G, "Isolation & antimicrobial susceptibility of Shigella from patients with acute gastroenteritis in Western Nepal", Indian J Med Res, 123, pp.145~150 (2006)
- Schmid S, "Pathophysiology", pp.261~315 (1979), Springer-Verlag
- Tseng JT, "Mechanisms and spectrum of streptomycin resistance in a natural population of Pseudomonas aeruginosa", Antimicrob Agents Chemother, 2, pp.136~141 (1972)
- Ximenes J, "Activity of amikacin, gentamicin and kanamycin against Pseudomonas aeruginosa", Int Med Res, 4, pp.165~175 (1976)
- de Melo MA, "Effect of mucin on Campylobacter jejuni association and invasion on HEp-2 cells", Microb Pathog, 5, pp.71~76 (1988)
- Portnoy DA, "Role of hemolysin for the intracellular growth of Listeria monocytogenes", J Exp Med, 167, pp.1459~1471 (1988)
- Shaw JH, "Model for invasion of human tissue culture cells by Neisseria gonorrhoeae", Infect Immun, 56, pp.1625~1632 (1988)
- Drevets DA, "Gentamicin kills intracellular Listeria monocytogenes", Infect Immun, 62, pp.2222~2228 (1994)
- Ohya S, "Killing mechanism of Listeria monocytogenes in activated macrophages as determined by an improved assay system", J Med Microbiol, 47, pp.211~215 (1998)
- Francis G, "The biological action of saponins in animal systems: a review", Br J Nutr, 88, pp.587~605 (2002)
- Armstrong JA, "Response of cultured macrophages to Mycobacterium tuberculosis, with observations on fusion of lysosomes with phagosomes", J Exp Med, 134(3 Pt 1), pp.713~740 (1971)
- Herbst S, "Interferon gamma activated macrophages kill mycobacteria by nitric oxide induced apoptosis", PLoS One, 6, pp.e19105~ (2011)
- Drevets DA, "Roles of complement and complement receptor type 3 in phagocytosis of Listeria monocytogenes by inflammatory mouse peritoneal macrophages", Infect Immun, 59, pp.2645~2652 (1991)
- Drevets DA, "Listericidal and nonlistericidal mouse macrophages differ in complement receptor type 3-mediated phagocytosis of L. monocytogenes and in preventing escape of the bacteria into the cytoplasm", J Leukoc Biol, 52, pp.70~79 (1992)
- Utermöhlen O, "Severe impairment in early host defense against Listeria monocytogenes in mice deficient in acid sphingomyelinase", J Immunol, 170, pp.2621~2628 (2003)
- Sharma L, "Assessment of phagocytic activity of cultured macrophages using fluorescence microscopy and flow cytometry", Methods Mol Biol, 1172, pp.137~145 (2014)
- Kaneko M, "Listeria monocytogenes: incidence, growth behavior and control", pp.153~163 (2015), Nova Science Publishers
- Peck R, "A one-plate assay for macrophage bactericidal activity", J Immunol Methods, 82, pp.131~140 (1985)
- Mancuso P, "Disruption of leptin receptor-STAT3 signaling enhances leukotriene production and pulmonary host defense against pneumococcal pneumonia", J Immunol, 186, pp.1081~1090 (2011)
- Domingo-Gonzalez R, "Prostaglandin E2-induced changes in alveolar macrophage scavenger receptor profiles differentially alter phagocytosis of Pseudomonas aeruginosa and Staphylococcus aureus post-bone marrow transplant", J Immunol, 190, pp.5809~5817 (2013)
