평면 소자형 락테이트 바이오센서
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Abstract
Two electrode-based lactate biosensor was prepared by immobilizing lactate oxidase (LOD) obtainedfrom pediococcus species in a poly(vinyl alcohol). Hydrogen peroxide (H2O2) produced by the reaction of lactateand LOD was detected on the Pt-black that was electrochemically deposited on the Au electrode. Sensorsfabricated with Pt-black deposited Au electrode provided a high current of H2O2 oxidation at a substantiallylowered applied potential (+300 mV vs. Ag/AgCl), resulting in reduced interferences from easily oxidizablespecies such as ascorbic acid, acetaminophen, and uric acid. An outer membrane is formulated by adjustingwater uptake of hydrophilic polyurethane (HPU). The sensor performance was evaluated in vitro with bothflow-through arrangement and static mode. The sensor showed a linear range from 0.1 mM to about 9.0 mMin 0.05 M phosphate bufer (pH 7.6) containing 0.05 M NaCl. Storing the sensors prepared in this work at4oC bufer solution while not in use, they provided same electrochemical performance for more than 25 days.
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