갈락토오즈 측정을 위한 전기화학적 바이오센서 개발

Abstract

In principle, the blood galactose level may be determined conveniently with a strip-type biosensorsimilar to that for glucose. In this study, we describe the development of a disposable galactose biosensor stripfor point-of-care testing. The sensor strip is constructed with screen-printed carbon paste electrode (SPCE) andsample amount (<100 L). The developed strip the galactose level in less than 90 s using bienzymatic systemof galactose oxidase (GAO) and horseradish peroxidase (HRP). The effects of pH, mediator (1,1-glassy carbon electrodes, and optimized further with the strip-type electrodes. The sensor exhibits linear responsein the range of 0~400 M (r2 = 0.997, S/N = 3). Since a low working potential, in principle, the fabricateddisposable galactose biosensor has -100 mV (vs. Ag/AgCl), it is applied for the detection of galactose, interferingresponses from common interferents such as ascorbic acid, uric acid and acetaminophen could be minimized.. Corresponding authorPhone : +82-(0)2-940-5246 Fax : +82-(0)2-91-8584E-mail : namh@kw.ac.kr394 ......... .............Analytical Science & TechnologyThe sensor has been used to determine the total galactose level in standard samples with satisfactoryreproducibility (CV = 5 %).