Simultaneous determinations of anthracycline antibiotics by high performance liquid chromatography coupled with radial-flow electrochemical cell

Abstract

.The analytical method of HPLC with the radial-flow electrochemical cell (RFEC) has been developedto determine doxorubicin, epirubicin, nogalamycin, daunorubicin and idarubicin simultaneously by employingreference electrode, a potential of diffusion curent plateau in the mobile phase. At a Vf of 1.0 mL/mindoxorubicin, epirubicin, daunorubicin and idarubicin appeared at a retention time (tr) of 6.4 min, 7.4 min, 12.7min and 18.4 min, respectively, while at a Vf of 0.6 mL/min, doxorubicin, epirubicin, nogalamycin, daunorubicinand idarubicin appeared at a tr of 9.9 min, 11.5 min, 13.5 min, 19.6 min and 28.7 min, respectively. The linearity. Corresponding authorPhone : +82-(0)2-2287-5144 Fax : +82-(0)2-2287-0070E-mail: yhahn@smu.ac.krAnalyses of anthracyclines by HPLC/RFEC 309Vol. 20, No. 4, 2007between each anthracycline injected (2.40×107 M~1.42×105 M) and peak area (charge) was excellent with thesquare of the correlation coefficient (R2) higher than 0.999. The detection limits were 1.0× 108 M~1.5× 107 Mfor the five anthracyclines. Within-day precision for the five anthracyclines were in reasonable relative standarddeviations less than 3 % (1.0