Effects of Interleukin-17A on the Early Stages of Arterial Thrombosis in Mice
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Purpose: Interleukin (IL)-17A has been suggested to play a role in the growth and organization of thrombi. We examined whetherIL-17A plays a role in the early stages of thrombosis and whether there are sex differences in the effects of IL-17A. Materials and Methods: We performed a blinded, randomized, placebo-controlled study to compare time to thrombotic occlu sion and sex differences therein between mice treated with IL-17A and those treated with saline using a ferric chloride-inducedmodel. We also assessed thrombus histology, blood coagulation, and plasma levels of coagulation factors. Results: Time to occlusion values did not differ between the IL-17A group and the control group (94.6±86.9 sec vs. 121.0±84.4 sec,p=0.238). However, it was significantly shorter in the IL-17A group of female mice (74.6±57.2 sec vs. 130.0±76.2 sec, p=0.032). Inrotational thromboelastometry, the IL-17A group exhibited increased maximum clot firmness (71.3±4.5 mm vs. 66.7±4.7 mm,p=0.038) and greater amplitude at 30 min (69.7±5.2 mm vs. 64.5±5.3 mm, p=0.040) than the control group. In Western blotting,the IL-17A group showed higher levels of coagulation factor XIII (2.2±1.5 vs. 1.0±0.9, p=0.008), monocyte chemoattractant pro tein-1 (1.6±0.6 vs. 1.0±0.4, p=0.023), and tissue factor (1.5±0.6 vs. 1.0±0.5, p=0.003). Conclusion: IL-17A plays a role in the initial st ages of arterial thrombosis in mice. Coagulation factors and monocyte chemoat tractant protein-1 may be associated with IL-17A-mediated thrombosis.
Abstract
Purpose: Interleukin (IL)-17A has been suggested to play a role in the growth and organization of thrombi. We examined whetherIL-17A plays a role in the early stages of thrombosis and whether there are sex differences in the effects of IL-17A. Materials and Methods: We performed a blinded, randomized, placebo-controlled study to compare time to thrombotic occlu sion and sex differences therein between mice treated with IL-17A and those treated with saline using a ferric chloride-inducedmodel. We also assessed thrombus histology, blood coagulation, and plasma levels of coagulation factors. Results: Time to occlusion values did not differ between the IL-17A group and the control group (94.6±86.9 sec vs. 121.0±84.4 sec,p=0.238). However, it was significantly shorter in the IL-17A group of female mice (74.6±57.2 sec vs. 130.0±76.2 sec, p=0.032). Inrotational thromboelastometry, the IL-17A group exhibited increased maximum clot firmness (71.3±4.5 mm vs. 66.7±4.7 mm,p=0.038) and greater amplitude at 30 min (69.7±5.2 mm vs. 64.5±5.3 mm, p=0.040) than the control group. In Western blotting,the IL-17A group showed higher levels of coagulation factor XIII (2.2±1.5 vs. 1.0±0.9, p=0.008), monocyte chemoattractant pro tein-1 (1.6±0.6 vs. 1.0±0.4, p=0.023), and tissue factor (1.5±0.6 vs. 1.0±0.5, p=0.003). Conclusion: IL-17A plays a role in the initial st ages of arterial thrombosis in mice. Coagulation factors and monocyte chemoat tractant protein-1 may be associated with IL-17A-mediated thrombosis.
참고문헌 (40)
- Tomaiuolo M, "Regulation of platelet activation and coagulation and its role in vascular injury and arterial thrombosis", Interv Cardiol Clin, 6, pp.1~12 (2017)
- Kuijpers MJ, "Key role of platelet procoagulant activity in tissue factor-and collagen-dependent thrombus formation in arterioles and venules in vivo differential sensitivity to thrombin inhibition", Microcirculation, 15, pp.269~282 (2008)
- Jackson SP, "Arterial thrombosis--insidious, unpredictable and deadly", Nat Med, 17, pp.1423~1436 (2011)
- Owens AP 3rd, "Tissue factor and thrombosis : the clot starts here", Thromb Haemost, 104, pp.432~439 (2010)
- Esmon CT, "The interactions between inflammation and coagulation", Br J Haematol, 131, pp.417~430 (2005)
- Swystun LL, "The role of leukocytes in thrombosis", Blood, 128, pp.753~762 (2016)
- de Boer OJ, "Neutrophils, neutrophil extracellular traps and interleukin-17 associate with the organisation of thrombi in acute myocardial infarction", Thromb Haemost, 109, pp.290~297 (2013)
- Ruf W, "Neutrophils release brakes of coagulation", Nat Med, 16, pp.851~852 (2010)
- Gould TJ, "Neutrophil extracellular traps promote thrombin generation through platelet-dependent and platelet-independent mechanisms", Arterioscler Thromb Vasc Biol, 34, pp.1977~1984 (2014)
- Peshkova AD, "Activated monocytes enhance platelet-driven contraction of blood clots via tissue factor expression", Sci Rep, 7, pp.5149~ (2017)
- Brill A, "Neutrophil extracellular traps promote deep vein thrombosis in mice", J Thromb Haemost, 10, pp.136~144 (2012)
- Fuchs TA, "Neutrophil extracellular trap(NET)impact on deep vein thrombosis", Arterioscler Thromb Vasc Biol, 32, pp.1777~1783 (2012)
- Park H, "Histological features of intracranial thrombi in stroke patients with cancer", Ann Neurol, 86, pp.143~149 (2019)
- Lee KO, "Effect of leukopenia induced by cyclophosphamide on the initial stage of arterial thrombosis in mice", Thromb Res, 206, pp.111~119 (2021)
- Shebuski RJ, "Role of inflammatory mediators in thrombogenesis", J Pharmacol Exp Ther, 300, pp.729~735 (2002)
- Ding P, "IL-17A promotes the formation of deep vein thrombosis in a mouse model", Int Immunopharmacol, 57, pp.132~138 (2018)
- Maione F, "Interleukin-17A exacerbates ferric chloride-induced arterial thrombosis in rat carotid artery", Int J Inflam, 2014, pp.247503~ (2014)
- Bouchnita A, "Modeling of the effects of IL-17 and TNF-α on endothelial cells and thrombus growth", C R Biol, 340, pp.456~473 (2017)
- Pu Y, "IL-17A upregulates expression of endothelial tissue factor in liver cirrhosis via the ROS", p38 signal pathway, 2016, pp.470, 41~47
- Marzona I, "Sex differences in stroke and major adverse clinical events in patients with atrial fibrillation : a systematic review and meta-analysis of 993, 600 patients", Int J Cardiol, 269, pp.182~191 (2018)
- WagstaffAJ, "Is female sex a risk factor for stroke and thromboembolism in patients with atrial fibrillation? A systematic review and meta-analysis", QJM, 107, pp.955~967 (2014)
- Raccah BH, "Gender differences in efficacy and safety of direct oral anticoagulants in atrial fibrillation : systematic review and network meta-analysis", Ann Pharmacother, 52, pp.1135~1142 (2018)
- Ramanathan R, "Sex difference in clot lysability and association to coronary artery calcification", Biol Sex Differ, 9, pp.9~ (2018)
- Zuern CS, "Platelet function and response to aspirin : gender-specific features and implications for female thrombotic risk and management", Semin Thromb Hemost, 35, pp.295~306 (2009)
- Farkouh A, "Sex-related differences in drugs with anti-inflammatory properties", J Clin Med, 10, pp.1441~ (2021)
- Leng XH, "Platelets of female mice are intrinsically more sensitive to agonists than are platelets of males", Arterioscler Thromb Vasc Biol, 24, pp.376~381 (2004)
- Kwon I, "Thrombolytic effects of the snake venom disintegrin saxatilin determined by novel assessment methods : a FeCl3-induced thrombosis model in mice", PLoS One, 8, pp.e81165~ (2013)
- Görlinger K, "Management of bleeding patients", pp.37~64 (2016), Springer International Publishing
- Wolberg AS, "Thrombin generation, fibrin clot formation and hemostasis", Transfus Apher Sci, 38, pp.15~23 (2008)
- Byrnes JR, "Factor XIIIa-dependent retention of red blood cells in clots is mediated by fibrin α-chain crosslinking", Blood, 126, pp.1940~1948 (2015)
- Weisel JW, "Fibrin formation, structure and properties", Subcell Biochem, 82, pp.405~456 (2017)
- Ylä-Herttuala S, "Expression of monocyte chemoattractant protein 1 in macrophage-rich areas of human and rabbit atherosclerotic lesions", Proc Natl Acad Sci U S A, 88, pp.5252~5256 (1991)
- Nishiyama K, "Simultaneous elevation of the levels of circulating monocyte chemoattractant protein-1 and tissue factor in acute coronary syndromes", Jpn Circ J, 62, pp.710~712 (1998)
- Schecter AD, "Tissue factor is induced by monocyte chemoattractant protein-1 in human aortic smooth muscle and THP-1 cells", J Biol Chem, 272, pp.28568~28573 (1997)
- Libby P, "Inflammation and thrombosis : the clot thickens", Circulation, 103, pp.1718~1720 (2001)
- Grover SP, "Tissue factor : an essential mediator of hemostasis and trigger of thrombosis", Arterioscler Thromb Vasc Biol, 38, pp.709~725 (2018)
- Morrissey JH, "Tissue factor : an enzyme cofactor and a true receptor", Thromb Haemost, 86, pp.66~74 (2001)
- Hot A, "Combination of IL-17 and TNFα induces a pro-inflammatory, pro-coagulant and pro-thrombotic phenotype in human endothelial cells", Ann Rheum Dis, 71, pp.768~776 (2012)
- Gremmel T, "Sex differences of leukocyte-platelet interactions and on-treatment platelet reactivity in patients with atherosclerosis", Atherosclerosis, 237, pp.692~695 (2014)
- James AH, "Pregnancy, contraception and venous thromboembolism(deep vein thrombosis and pulmonary embolism)", Vasc Med, 22, pp.166~169 (2017)
