A study on the analysis of artificial sweeteners in processed foods including emulsified foods by HPLC-DAD
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초록
This study has been carried out to develop a standard method for quantifying of 3 permitted artificial sweeteners (including sodium saccharine, aspartame, acesulfame potassium) contained in foods by HPLC-DAD. A simple and rapid sample pretreatment method was used to remove fat and protein from the test solution with Carrez clearing regent precipitant know to be effective for protein and fat removal. The artificial sweeteners in the test solution purified through sample pretreatment were detected by high performance liquid chromatograph using a Reverse phase C18 column (5 μm, 4.6 × 250 mm). The simultaneous quantitative test of 3 kinds of artificial sweeteners can be effectively performed on the high fat emulsified foods containing a large amount of fat. Using the established simultaneous quantitative test method, artificial sweeteners were tested in foods such as dairy products, snacks and chocolate. The results calibration curve showed good linearity with high regression coefficients and the result of recovery test showed satisfactory recoveries within 80~110 %.
Abstract
This study has been carried out to develop a standard method for quantifying of 3 permitted artificial sweeteners (including sodium saccharine, aspartame, acesulfame potassium) contained in foods by HPLC-DAD. A simple and rapid sample pretreatment method was used to remove fat and protein from the test solution with Carrez clearing regent precipitant know to be effective for protein and fat removal. The artificial sweeteners in the test solution purified through sample pretreatment were detected by high performance liquid chromatograph using a Reverse phase C18 column (5 μm, 4.6 × 250 mm). The simultaneous quantitative test of 3 kinds of artificial sweeteners can be effectively performed on the high fat emulsified foods containing a large amount of fat. Using the established simultaneous quantitative test method, artificial sweeteners were tested in foods such as dairy products, snacks and chocolate. The results calibration curve showed good linearity with high regression coefficients and the result of recovery test showed satisfactory recoveries within 80~110 %.
참고문헌 (24)
- "식품공전" (2016), 식품의약품안전처[URL]
- 최성희, "한국인의 인공감미료 섭취수준 평가", 한국식품과학회지, 43(3), pp.387~395 (2011), 한국식품과학회
- S. Chattopadhyay, "Artificial sweeteners-a review", J. Food Sci. Technol, 51, pp.611~621 (2014)
- Food additives in food analysis contained in the MFDS, , ~ (2016)
- "식품첨가물공전" (2016), 식품의약품안전처[URL]
- H. -Y. Kim, , Korean. J. Food. Sci. Technol, 36(1), pp.14~18 (2004)
- 서희재, "한국인의 아스파탐과 아세설팜 칼륨의 식이 섭취 수준 평가", 한국식품영양과학회지, 43(4), pp.604~611 (2014), 한국식품영양과학회
- 이성봉, "HPLC", MS, 한국식품위생안전성학회지, pp.2014, 29(4)[DOI]
- 김일영, "HPLC-MS", MS를 이용한 어린이 기호식품중의 감미료 분석, 2010, pp.25(2), 118~121[DOI]
- S. K. Jung, "Simultaneous Determination of Artificial Sweeteners in Foods by HPLC", 한국법과학회 학술대회, pp.105~106
- "Foodstuffs-Determination of acesulfame-K, aspartame and saccharin-High performance liquid chromatographic method", EN method BS EN 12856:1999, ~ (1999)
- "Determination of cyclamate by HPLC method", EN method BS EN 12857:1999, ~ (1999)
- J. F. Lawrence, , Journal of the Association of Official Analytical Chemists, 71(5), pp.934~937 (1988)
- D. de Queiroz Pane, , Journal of Food Science and Technology, 52(11), pp.6900~6913 (2015)
- 임호수, "HPLC-MS", MS Analysis of 9 Artificial Sweeteners in Imported Foods, 2013, pp.22, 233~240[DOI]
- A. B. Bergamo, , Food Chemistry, 124(4), pp.1714~1717 (2011)
- H. Matsumoto, , Journal of the Food Hygienic Society of Japan, 49(1), pp.31~36 (2018)
- Y. Zhu, , Journal of Chromatography A, 1085(1), pp.143~146 (2005)
- Q. H. Yan, , Journal of Liquid Chromatography & Related Technologies, 35(2), pp.268~279 (2012)
- M. J. Scotter, , Food Additives and Contaminants, 26(5), pp.614~622 (2009)
- ISO, International Sugar Organization, "Alternative sweeteners in a high sugar price environment 2012", Mark. Eval. Consum. Stat. Comm, 1~53 (2012)
- H. Sakai, , Food Additives & Contaminants : Part A, 32(6), pp.808~816 (2015)
- A. Elhassan, , New York Science Journal, 10, pp.121~128 (2017)
- D. J. Yang, , Food Chem, 57, pp.3022~3027 (2009)