Optimization of an extraction method for the simultaneous quantification of six active compounds in the aril part of Orostachys japonicus using HPLC–UV
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In this study, we describe the development of a new high-performance liquid chromatography (HPLC) method for the simultaneous analysis of six bioactive compounds (including gallic acid, epicatechin 3-gallate, quercitrin, afzelin, quercetin, and kaempferol) from Orostachys japonicus. The extraction method was investigated and optimization of the extraction time (min), solvent composition (%), and solvent to material ratio were conducted. As a result, 30 min extraction with 50% methanol and 40:1 mL/g of solvent: material ratio achieved the highest extraction efficiency with a yield of 3.32 mg/g. Furthermore, the developed HPLC method was validated and the correlation coefficient (R) values were within the satisfactory range of 0.9995-0.9999 over the linearity range of 1.53-417 μg/mL. The limit of detection and limit of quantification for the six active components were between 0.03-0.08 μg/mL and 0.08-0.26 μg/mL, respectively. With these newly optimized and developed methods, four batches of O. japonicus were analyzed to confirm the high extraction efficiency of the method and the feasibility of an application.
Abstract
In this study, we describe the development of a new high-performance liquid chromatography (HPLC) method for the simultaneous analysis of six bioactive compounds (including gallic acid, epicatechin 3-gallate, quercitrin, afzelin, quercetin, and kaempferol) from Orostachys japonicus. The extraction method was investigated and optimization of the extraction time (min), solvent composition (%), and solvent to material ratio were conducted. As a result, 30 min extraction with 50% methanol and 40:1 mL/g of solvent: material ratio achieved the highest extraction efficiency with a yield of 3.32 mg/g. Furthermore, the developed HPLC method was validated and the correlation coefficient (R) values were within the satisfactory range of 0.9995-0.9999 over the linearity range of 1.53-417 μg/mL. The limit of detection and limit of quantification for the six active components were between 0.03-0.08 μg/mL and 0.08-0.26 μg/mL, respectively. With these newly optimized and developed methods, four batches of O. japonicus were analyzed to confirm the high extraction efficiency of the method and the feasibility of an application.
참고문헌 (29)
- J. H. Lee, , Food Chem, 124(4), pp.1627~1633 (2011)
- 이수정, "건조 방법에 따른 와송의 항산화 효과", 한국식품영양과학회지, 37(5), pp.605~611 (2008), 한국식품영양과학회
- M. -J. Ko, , Sci. Rep, 10(1), pp.1~10 (2020)
- D. S. Im, , Acta Biochim. Biophys. Sin, 49(10), pp.945~953 (2017)
- J. C. Park, , J. Ethnopharmacol, 102(3), pp.313~318 (2005)
- Z. Zhou, , J. Ethnopharmacol, 265, pp.113392~ (2021)
- G. Das, , Plos One, 12(7), pp.e0181280~ (2017)
- H. Zhang, , Food Chem, 131(4), pp.1097~1103 (2012)
- H. -S. Lee, , J. Funct. Foods, 5(3), pp.1217~1225 (2013)
- 김세건, "Anti-obesitic Effect of Orostachys japonicus in Rats Model fed a Hyperlipidemic Diet", Natural Product Sciences, 17(2), pp.117~122 (2011), 한국생약학회
- J. H. Kim, , Int. J. Biol. Macromol, 91, pp.1033~1039 (2016)
- M. -H. Chen, , J. Ethnopharmacol, 181, pp.193~213 (2016)
- X. -M. Wang, , Fitoterapia, 83(8), pp.1576~1584 (2012)
- H. J. Yoo, , J. Pharm. Pharmacol, 60(6), pp.779~786 (2008)
- T. Khezeli, , Talanta, 150, pp.577~585 (2016)
- Rohit Upadhyay, "Ultrasound-Assisted Extraction of Flavonoids and Phenolic Compounds from Ocimum tenuiflorum Leaves", Food Science and Biotechnology, 24(6), pp.1951~1958 (2015), 한국식품과학회[DOI]
- J. Li, , Prep. Biochem. Biotechnol, 49(1), pp.21~29 (2019)
- Q. Niu, , Prep. Biochem. Biotechnol, 50(1), pp.82~90 (2020)
- S. Deng, , J. Chromatogr. B, 877(24), pp.2487~2492 (2009)
- N. Sendi, , Prep. Biochem. Biotechnol, 50(9), pp.943~953 (2020)
- "ICH Guideline. Q2 (R1): Validation of Analytical Procedure: Text and Methodology (Q2) (R1) in ICH Hamonised Tripartite Guideline", ~ (2005)
- D. Gao, , Plants, 9(11), pp.1586~ (2020)
- Gao Dan, "Discrimination and quality evaluation of fifteen components in Stauntonia hexaphylla leaves from different harvest time by HPLC–PDA–ESI–MS", MS and ELSD coupled with multivariate statistical analysis and anti-inflammatory activity evaluation, 2020, pp.63(5), 1~11[DOI]
- D. Gao, , Acta Chromatogr, 33(4), pp.371~377 (2021)
- Gao Dan, "Simultaneous qualitative and quantitative analysis of morroniside and hederacoside D in extract mixture of Cornus officinalis and Stauntonia hexaphylla leaves to improve benign prostatic hyperplasia by HPLC-UV", 분석과학, 33(5), pp.224~231 (2020), 한국분석과학회
- A. Pandey, , Ind. Crops Prod, 119, pp.218~225 (2018)
- A. Arvindekar, , Ind. Crops Prod, 83, pp.587~595 (2016)
- F. Chemat, , Ultrason. Sonochem, 18(4), pp.813~835 (2011)
- A. Spietelun, , Chem. Rev, 113(3), pp.1667~1685 (2013)