고체상 추출과 GC/MS를 이용한 소변 중 대마대사체(THCCOOH) 분석
Determination of 11-nor-9-carboxy-△9-tetrahydrocannabinol(THCCOOH) in human urine by solid-phase extraction and GC/MS
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Abstract
1-nor-9-carboxy-9(THC) which is the primary psychoactive component of marijuana. It is also the target analyte for thediscrimination marijuana use. A method using solid-phase extraction (SPE) and gas chromatography/massspectrometry (GC/MS) was developed for the determination of THCCOOH in human urine. Urine samples(3 mL) were extracted by SPE column with a cation exchange cartridge after basic hydrolysis. The eluentswere then evaporated, derivatized, and injected into the GC/MS. The limits of detection (LOD) and quantitation. Corresponding authorPhone : +82-(0)2-535-4173 Fax : +82-(0)2-535-4175E-mail: saturn-jjc@spo.go.kr, jc2661_2000@naver.com442 ............ ...Analytical Science & Technology(LOQ) were 0.4 and 1.2 ng/mL, respectively. The response was linear with a correlation coefficient of 0.9within the concentration range of 1.2 (LLE 1.3)~50.0 ng/mL. The precision and accuracy were stable within1.20% and the recovery was 83.6~90.7%. The recovery of SPE method was lower than that of liquid-liquidextraction (LLE), but there were no apparent differences in LOD, LOQ, precision and accuracy between thetwo methods. While SPE method is used as a very effective and rapid procedure for sample pretreatment, andclean extracts, LLE method was not suitable for the extraction procedure of THCCOOH in urine. Theapplicability of the method was proven by analyzing a urine samples from a marijuana abusers.
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