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Duplex SDS-PAGE를 이용한 단백질 분리향상

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주제분류화학

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Abstract

The protein separation with molecular weight using SDS-PAGE(sodium dodecyl sulfate-polyacrylamidegel electrophoresis) is the one of the most conventional and simple techniques. In, this study, two dimensionalSDS-PAGE using same separation principle consecutively was investigated and compared with one dimensionalSDS-PAGE. The enhanced separation from duplex SDS-PAGE was observed and separated proteins in the gelwere identified by MALDI TOF MS. Identified proteins from diferent gel spots were found to have differentgi numbers. Therefore, duplex SDS-PAGE separation method will be used for economic separation method in

참고문헌 (8)

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