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Effect of Solvent on the Surface of Protein Chip Plate

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주제분류기계공학

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Abstract

Nickel chloride coated protein chip plate was developed by using a spin coating method. The ability of histidine tagged protein adsorption was investigated at various solvents. The surface of plate has a large aggregated nickel complex with high density in water. However, the surface of plate has a very small size of aggregated nickel complex with low density in isopropanol. The ability of protein adsorption decreased as increasing the size of alkyl chain in various alcohol solvents. The mechanism on the ability of protein adsorption at the plate surface is discussed.

참고문헌 (4)

  1. E. T. Fung, "Protein Biochips for Differential Profiling. Current Opinion in Biotechnology", 12, pp.65~ (2001)
  2. G. MacBeath, "Printing Proteins as Microarrays for High-throughput Function Determina- tion. Science", 289, pp.1760~ (2000)
  3. D. V. Nicolau, "Micron-sized Protein Patterning on Diazonaphthoquinone", novolak Thin Polymeric Films. Langmuir, 19981927., pp.14, 1927~
  4. R. Bashir, "Adsorption of Avidin on Microfabricated Surfaces for Protein Biochip Applications. Biotechnol. Bioeng.", 73, pp.324~ (2001)

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