어린이 제품 중 가스 크로마토그래피를 이용한 알러지 유발 방향성 물질의 동시분석법
Simultaneous determination of aromatic material causing allergic in children’s products by Gas Chromatography
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어린이 제품 중 22종의 알러지 유발 방향성 물질들을 기체 크로마토그래피 불꽃 이온화 검출기(GC-FID)와 기체 크로마토그래피 질량 분석기(GC-MSD)를 사용하여 분석하였다. 분석물질들은 자동속슬렛 추출장치를 사용하여 추출하였으며, 고속 냉동 원심분리기에서 10분동안 원심분리 후 상징액을 2 mL vial 옮겨 Split mode로 주입하여 분석하였다. 확립된 조건에서 검정곡선은 0.996 이상의 상관계수를 갖는 직선성을 보였으며, 감도의 경우는 6.7~1,859,839로서 물질별로 기기특성 및 검출기에 따라 상당히 넓은 범위의 감도를 나타내었다. 기기검출한계는 0.0032~0.0335 μg/mL로서 최대 약 0.1 μg/ mL이하의 기기검출한계를 나타내었고, 방법검출한계는 0.0033~0.1161 μg/mL의 범위를 나타내었다. 또한정량한계는 0.0100~0.5422 μg/mL의 범위를 보였고, 정밀도는 0.21~4.89%, 정확도는 89~111%의 범위를 나타내었다. 본 연구에서 개발한 분석법으로 시판품에 실제 적용하였다.
Abstract
Twenty-two allergy-induced aromatics in children were analyzed using a gas chromatography flame ionization detector (GC-FID) and gas chromatography mass spectrometer (GC-MSD). Analytes were extracted using an automatic Soxhlet extractor and centrifuged for 10 min in a fast freezing centrifuge, and the supernatant was transferred into a 2 mL vial and injected in split mode. Under the established conditions, the calibration curve showed linearity with a correlation coefficient of 0.996 or more. A wide range of sensitivity of 6.7 to 1,859,839 depending on the device characteristics and detector used was shown. The detection limit of the device was 0.0032 to 0.0335 μg/mL, and the maximum detection limit was less than 0.1 μg/mL. The detection limit of the method ranged from 0.0033 to 0.1161 μg/mL. In addition, the limit of quantification ranged from 0.0100 to 0.5422 μg/mL, with a level of precision ranging from 0.21 % to 4.89 % and a degree of accuracy ranging from 89 % to 111 %. The analytical method developed in this study was applied to commercial products.
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