GC-MSD를 이용한 어린이 제품 중 알러지 유발 방향성 물질의 동시분석법 연구
Study on simultaneous determination of aromatic material causing allergic in children’s products by GC-MSD
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어린이 제품 중 21종의 알러지 유발 방향성 물질들을 기체 크로마토그래피 질량 분석기(GCMSD) 를 사용하여 분석하였다. 분석물질들은 자동 속슬렛 추출장치를 사용하여 추출하였으며, 고속 냉동원심분리기에서 10분동안 원심분리하고 상징액을 실린지 필터로 여과 한 후 2 mL vial에 옮겨 Split mode 로 주입하여 분석하였다. 확립된 조건에서 검정곡선은 0.9981 이상의 결정계수를 갖는 직선성을 보였으며, 감도의 경우는 0.3145 ~ 1.6757로서 물질별로 상당히 넓은 범위의 감도를 나타내었다. 기기검출한계는 0.0016 ~ 0.0423 μg/mL로서 최대 약 0.05 μg/mL이하를 나타내었고, 방법검출한계는 0.0030 ~ 0.0589 μg/ mL의 범위를 나타내었다. 또한 정량한계는 0.0096 ~ 0.1876 μg/mL의 범위를 보였고, 정밀도는0.41 ~ 10.49%, 정확도는 83 ~ 116%의 범위를 나타내었다. 본 연구에서 개발한 분석법으로 시판품에 실제 적용하였다.
Abstract
Twenty-one allergy-induced aromatic material in children’s products were analyzed using gas chromatography mass spectrometer(GC-MSD). The analytes were extracted using an automatic Soxhlet extractor, centrifuged for 10 minutes in a fast freezing centrifuge, and the supernatant was filtered with a syringe filter and then transferred into a 2 mL vial and injected in a split mode. In the established condition, the calibration curve showed linearity with a determination coefficient of 0.9981 or more. Sensitivity was 0.3145 ~ 1.6757, which showed a fairly wide range of sensitivity for each substance. The detection limit of the device was 0.0016 ~ 0.0423 μg/mL and the maximum detection limit was less than 0.05 μg/mL. The method detection limit ranged from 0.0030 ~ 0.0589 μg/mL. In addition, the limit of quantification ranged from 0.0096 to 0.1876 μg/mL, with precision ranging from 0.41 to 10.49 % and accuracy ranging from 83 to 116 %. The analytical method developed in this study was applied to commercial products.
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