Calcium Phosphate Graft Material이 적용된 발치와의 치유 양상에 대한 조직학적 연구
Histological Study of Extraction Socket Grafted with Calcium Phosphate Materials: An Animal Study
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본 연구의 목적은 인산칼슘제제인 MBCPⓇ, PolyboneⓇ을 발치와에 이식 후 치유 양상을 조직학적으로 관찰하는 것이다. 비글견의 소구치를 발치 후 발치와에 골이식재를 이식하였으며, 3개월간의 치유기간을 거친 후 조직표본을 제작하여 현미경으로 치유 양상을 관찰하였다. MBCPⓇ, PolyboneⓇ은 조직학적으로 비슷한 치유 양상을 보였으며 이물반응이나 염증 반응 없이 신생골 생성을 유도하여 정상적인 치유를 도모하였다. 치유된 조직의 조직 계측학적 평가에서 신생골 비율은 골이식재를 이식하지 않은 대조군이 34.5%, MBCPⓇ는 28.4%, PolyboneⓇ은 23.8%였으며, 골이식재를 포함한 전체 mineralized tissue의 비율은 각각 34.5%, 42.9%, 37.3% 였다. 이상의 결과에서 MBCPⓇ, PolyboneⓇ은 발치와에 사용할 골이식재로 가치가 있는 것으로 생각된다.
Abstract
The purpose of this study was to evaluate histologic result of bone substituting materials on extraction sockets. We compare the histologic findings of control, MBCPⓇ, PolyboneⓇ. Mandibular premolar teeth of Beagle dogs were extracted available for bone filling. All alveolar extraction sockets were thoroughly debrided with surgical curet to remove the periodontal ligament. The graft materials were filled into the extraction sockets. The animals were sacrified 90 days after implantation. Both treated and control mandibular sites were histologically evaluated with light microscopy. Histological observation at 90 days revealed that control and experimental sites were healed uneventfully without any adverse tissue reaction. Regenerated new bone formation ratio is 34.5% for control, 28.4% for MBCPⓇ, 23.8% for PolyboneⓇ. From this results, it was suggested that MBCPⓇ, PolyboneⓇ are promising bone substituting materials to promote normal tissue healing and new bone formation.
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