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Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against experimental Neospora caninum infection

김동수|송경주|정우석|강승원|나병국|송철용|조정화
대한기생충학ㆍ열대의학회 (The Korean Society For Parasitology and Tropical Medicine)|2005.03|Parasites, Hosts and Diseases|제43권 제1호|pp. 1-21

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주제분류예방의학/직업환경의학

초록

Abstract

Protective efficacy of vaccination with Neospora caninum multiple recombinant antigens against N. caninum infection was evaluated in vitro and in vivo. Two major immunodominant surface antigens (NcSAG1 and NcSRS2) and two dense granule proteins (NcDG1 and NcDG2) of N. caninum tachyzoites were expressed in E. coli, respectively. An in vitro neutralization assay using polyclonal antisera raised against each recombinant antigen showed inhibitory effects on the invasion of N. caninum tachyzoites into host cells. Separate groups of gerbils were immunized with the purified recombinant proteins singly or in combinations and animals were then challenged with N. caninum infection. Following these experimental challenges, the protective efficacy of each vaccination was determined by assessing animal survival rate. All experimental groups showed protective effects of different degrees against experimental infection. The highest protection efficacy was observed for combined vaccination with NcSRS2 and NcDG1. Our results indicate that combined vaccination with the N. caninum recombinant antigens, NcSRS2 and NcDG1, induces the highest protective effect against N. caninum infection in vitro and in vivo, and suggest that they should be considered as a desirable combination for N. caninum vaccine development.

참고문헌 (14)

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  8. "The p29 and p35 immunodominant antigens of Neospora caninum tachyzoites are homologous to the family of surface antigens of Toxoplasma gondii", 66, pp.5322~5328 (1998)
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관련 논문AI추천

Review of Neospora caninum and neosporosis in animalsJ.P.DUBEY대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2003 · 제41권 제1호Loss of infectivity of Neospora caninum oocysts maintained for a prolonged timeAlexandre Moraes Pinheiro, Kattyanne De Souza Costa, Luis Fernando Pita Gondim, Maria Angela Ornelas De Almeida, Milton M. McAllister, Rosangela Soares Uzeda, Sara Lima Santos대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2007 · 제45권 제4호Vaccination against Murine Toxoplasmosis Using Recombinant Toxoplasma gondii SAG3 Antigen Alone or in Combination with Quil A남호우, 신대완, 안명희, 이영하, 이재호연세대학교의과대학 · Yonsei Medical Journal2007 · 제48권 제3호ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine seraDae-YongKIM, Ho-WooNAM, Hye-JinAHN, SeraKIM대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2003 · 제41권 제3호Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum현학남, Li-Jun Jia, Long-Zheng Yu, Ming-Ming Liu, Nian-Chao Qian, Shou-Fa Zhang, Xue-Mei Zhang대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2013 · 제51권 제2호Seroprevalence of antibodies to Neospora caninum in dogs and raccoon dogs in KoreaByung-ChunLEE, Byung-JaeSO, Chang-WooLee, Dae-YongKIM, Jae-HoonKIM, J.P.DUBEY, Min-SooKANG, Woo-SukHWANG대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2003 · 제41권 제4호

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