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Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum

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Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2,and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7,expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was 109TCID50/ml. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 1:4,096. IFN-γ and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.

Abstract

Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2,and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7,expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was 109TCID50/ml. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 1:4,096. IFN-γ and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.

참고문헌 (39)

  1. Dubey JP, "Newly recognized fatal protozoan disease of dogs", J Am Vet Med Assoc, 192, pp.1269~1285 (1988)
  2. Davison HC, "Isolation of bovine Neospora in Britain", Vet Rec, 141, pp.607~ (1997)
  3. Yamane I, "In viro isolation and characteristion of a bovine Neospora species in Japan", Rec Vet Sci, 63, pp.77~80 (1997)
  4. Dubey JP, "Neosporosis in cattle; biology and economic impact", J Am Vet Med Assoc, 214, pp.1160~1163 (1999)
  5. Thurmond MC, "Effect of Neospora caninum infection on milk production in first-lactation dairy cows", J Am Vet Med Assoc, 210, pp.672~674 (1997)
  6. Stenlund S, "Serum antibody profile and reproductive performance during two consecutive pregnancies of cows naturally infected with Neospora caninum", Vet Parasitol, 85, pp.227~234 (1999)
  7. Jenkins M, "Diagnosis and seroepidemiology of Neospora caninum-associated bovine abortion", Int J Parasitol, 32, pp.631~636 (2002)
  8. Cramer G, "Neospora caninum serostatus and culling of Holstein cattle", J Am Vet Med Assoc, 221, pp.1165~1168 (2002)
  9. Cañón-Franco WA, "Prevalence of antibodies to Neospora caninum in dogs from Amazon, Brazil", Vet Parasitol, 115, pp.71~74 (2003)
  10. Liu Q, "The first report on the serological test of Neosporosis in dairy cows", Chin J Vet Med, 39, pp.8~9 (2003)
  11. Chao WD, "Serological investigation of Neospora caninum infection in dairy cows in Geermu county of Qinghai province", Chin J Vet Sci Technol, 35, pp.1012~1014 (2005)
  12. Bayin CH, "Serologic tests with the recombinant NcSRS1 antigen on the cattles infected with Neospora caninum in Xinjiang partial areas", Chin J Vet Parasitol, 16, pp.10~12 (2008)
  13. Zhang SF, "The comparison of colloidal gold immunochromatography and ELISA used in the detection of Neospora caninum sera antibody", Chin J Vet Med, 44, pp.54~55 (2008)
  14. Wang CR, "Development and preliminary application of indirect ELISA diagnostic method for Neospora caninum in dairy cattle", Chin J Prev Vet Med, 31, pp.145~149 (2009)
  15. Deng C, "Identification of Neospora caninum in aborted fetus of dairy cow in China", Chin Vet Sci, 37, pp.16~19 (2007)
  16. Ding D, "Isolation and identification of Jilin strain Neospora caninum", Chin Vet Sci, 29, pp.423~425 (2009)
  17. Hemphill A, "Identification of a major surface protein on Neospora caninum tachyzoites", Parasitol Res, 82, pp.497~504 (1996)
  18. Hemphill A, "Characterization of a cDNA-clone encoding Nc-p43, a major Nesopora caninum tachyzoite surface protein", Parasitology, 115, pp.581~590 (1997)
  19. Sonda S, "The major 36 kDa Neospora caninum tachyzoite surface protein is closely related to the major Toxoplasma gondii surface antigen", Mol Biochem Parasitol, 97, pp.97~108 (1998)
  20. Hemphill A, "Subcellular localization and functional characterization of Nc-p43, a major Neospora caninum tachyzoite surface protein", Infect Immun, 64, pp.4279~4287 (1996)
  21. Howe DK, "The p29 and p35 immunodominant antigens of Neopora caninum tachyzoites are homologous to the family of surface antigens of Toxoplasma gondii", Infect Immun, 66, pp.5322~5328 (1998)
  22. Nishikawa Y, "Characterization of Neospora caninum surface protein NcSRS2 based on baculovirus expression system and its application for serodiagnosis of Neospora infection", J Clin Microbiol, 39, pp.3987~3991 (2001)
  23. Nishikawa Y, "Neospora caninum NcSRS2 is transmenbrane protein that contains a glycosylphosphatidylinositol anchor insect cells", Vet Parasitol, 109, pp.191~201 (2002)
  24. Nishikawa Y, "Prevention of vertical transmission of Neospora caninum in BALB", c mice by recombinant vaccinia virus carrying NcSRS2 gene, 2001, pp.19, 1710~1716
  25. Dubremetz JF, "Kinetics and pattern of organelle exocytosis during Toxoplasma gondii", host-cell interaction, 1993, pp.79, 402~408
  26. Jenkins M, "Inclusion of CpG adjuvant with plasmid DNA coding for NcGRA7 improves protection against congenital neosporosis", Infect Immun, 72, pp.1817~1819 (2004)
  27. Chahan B, "Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1", Vet Parasitol, 118, pp.177~185 (2003)
  28. Bruna-Romero O, "Enhanced protective immunity against malaria by vaccination with a recombinant adenovirus encoding the circumsporozoite protein of Plasmodium lacking the GPI-anchoring motif", Vaccine, 22, pp.3575~3584 (2004)
  29. Zhan XG, "Immunogenicity of a recombinant adenovirus expressing HA gene of H3N2 subtype swine influenza virus in mice", Scientia Agricultura Sinica, 43, pp.1235~1241 (2010)
  30. Lovett JL, "Molecular characterization of a thrombospondin-related anonymous protein homologue in Neospora caninum", Mol Biochem Parasitol, 107, pp.33~43 (2000)
  31. Huang P, "Densegranule protein NcGRA7, a new marker for the serodiagnosis of Neospora caninum infection in aborting cows", Clin Vaccine Immunol, 14, pp.1640~1643 (2007)
  32. Shott JP, "Adenovirus 5 and 35 vectors expressing Plasmodium falciparum circumsporozoite surface protein elicit potent antigen-specific cellular IFN-gamma and antibody responses in mice", Vaccine, 26, pp.2818~2823 (2008)
  33. Zhou D, "A chimpanzee-origin adenovirus vector expressing the rabies virus glycoprotein as an oral vaccine against inhalation infection with rabies virus", Mol Ther, 14, pp.662~672 (2006)
  34. Gong F, "Medical Immunology", pp.132~141 (2009), Science Press
  35. Cui Z, "Veterinary Immunology", pp.81~85 (2004), China Agriculture
  36. Cao LL, "Regulation of host Th1 immune response by the alarmins of Neospora caninum", pp.2010
  37. Wu XY, "Research progress on host cell invasion by Neospora caninum", Chin Bull Life Sci, 22, pp.873~877 (2010)
  38. Feng XS, "Separation and Investigation on Neospora caninum soluble complexes which Induce Th-1immune response", pp.2009
  39. Baszler TV, "Interferon-gamma and interleukin-12 mediate protection to acute Neospora caninum infection in BALB", c mice, 1999, pp.29, 1635~1646

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