공기 중 존재하는 진균에 의한 호산구의 활성화
Activation of Eosinophils with Airborne Fungi
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Abstract
Background and Objectives：Allergic fungal sinusitis is characterized by allergic mucin which includes eosinophils and fungal the pathophysiologic function of eosinophils by directly stimulating eosinophils with fungal antigens. Subjects and Method：Eosinophils were isolated from healthy volunters and stimulated with extracts from 5 comon fungal species (Alternaria, Aspergilus, Candida, Cladosporium, and Penicillium). Superoxide production and eosinophil derived neurotoxin (EDN) were measured to determine whether fungi activated eosinophils. An inhibition study was done using serine and cystein protease inhibitors. ：When cultured with fungal antigens, eosinophils produced superoxide by Alternaria and Cladosporium but only Alternaria induced EDN production. Serine protease inhibitors (PMSF, Pefabloc) and heat treatment of fungi significantly inhibited the activation of eosinophils but the cystein protease inhibitor (E-64) wasnt inhibited. Conclusion：Eosinophils are directly activated by Alternariamay activate eosinophils which play important roles in the pathogenesis of AFS, resulting in the destruction of fungi and respiratory epithelial cells.
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