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Cloning and expression of trypsin-encoding cDNA from Blattella germanica and its possibility as an allergen

김선미|김봉진|변강현|옥미선
대한기생충학ㆍ열대의학회 (The Korean Society For Parasitology and Tropical Medicine)|2005.09|Parasites, Hosts and Diseases|제43권 제3호|pp. 101-110

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참고문헌

주제분류예방의학/직업환경의학

초록

Abstract

In this study, the trypsin gene (bgtryp-1) from the German cockroach, Blattella germanica, was cloned viathe immunoscreening of patients with allergies to cockroaches. Nucleotide sequence analysis predicted an 863 bpopen reading frame which encodes for 257 amino acids. The deduced amino acid sequence exhibited 42-57% homol-ogy with the serine protease from dust mites, and consisted of a conserved catalytic domain (GDSGGPLV). bgtryp-1was determined by both Northern and Southern analysis to be a 0.9 kb, single-copy gene. SDS-PAGE and Westernblotting analyses of the recombinant protein (Bgtryp-1) over-expressed in Escherichia colirevealed that the molecularmass of the expressed protein was 35 kDa, and the expressed protein was capable of reacting with the sera of cock-roach allergy patients. We also discussed the possibility that trypsin excreted by the digestive system of the Germancockroach not only functions as an allergen, but also may perform a vital role in the activation of PAR-2.

참고문헌 (38)

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관련 논문AI추천

바퀴(Blattella germanica)에서 발현되는 세린계 단백분해효소 유전자군의 염기서열분석 및 알레르겐으로서의 가능성김현범, 박경원, 박성현, 박종률, 옥미선, 유태현, 이원용대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2008 · 제51권 제6호Reactivity of German Cockroach Allergen, Bla g 2, Peptide Fragments to IgE Antibodies in Patients’ Sera이해석대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2008 · 제46권 제4호IgE Binding Reactivity of Peptide Fragments of Bla g 4, a Major German Cockroach Allergen용태순, 정경용, 홍천수, Kwang Hyun Shin대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2009 · 제47권 제1호A Chymotrypsin Gene Homologue from the Mulberry Longicorn Beetle, Apriona germari: cDNA Sequence Characterization and mRNA Expression Pattern김익수, 손흥대, 윤형주, 이광식, 진병래, Zong Zheng Gui한국잠사학회 · International Journal of Industrial Entomology and Biomaterials2005 · 제11권 제2호Expressed sequence tags analysis of Blattella germanica김봉진, 김선미, 김주영, 옥미선, 유태현, 유학선, 정해진, 정형석대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2005 · 제43권 제4호Enzymatic Activities of Allergen Extracts from Three Species of Dust Mites and Cockroaches Commonly Found in Korean Home용태순, 정경용, Chungryul Kim대한기생충학ㆍ열대의학회 · Parasites, Hosts and Diseases2010 · 제48권 제2호

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