Antigenemia and Specific IgM and IgG Antibody Responses in Rabbits Infected with Toxoplasma gondii
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In this experiment, the correlation between antigenemia and specific antibody responses in Toxoplasma gondiiinfected rabbits was assessed. We injected 1,000 T. gondii tachyzoites (RH) subcutaneously into 5 rabbits. Parasitemia, circulating antigens, and IgM and IgG antibody titers in blood were tested by ELISA and immunoblot. For detection of parasitemia, mice were injected with blood from rabbits infected with T. gondii and mice died between days 2 and 10 post-infection (PI). Circulating antigens were detected early on day 2 PI, and the titers increased from day 4 PI and peaked on day 12 PI. Anti-Toxoplasma IgM antibody titers increased on day 6 PI and peaked on days 14-16 PI. IgG was detected from day 10 PI, and the titers increased continuously during the experiment. The antigenic protein patterns differed during the infection period, and the number of bands increased with ongoing infection by the immunoblot analysis. These result indicated that Toxoplasma circulating antigens during acute toxoplasmosis are closely related to the presence of parasites in blood. Also, the circulating antigen levels were closely correlated with IgM titers, but not with IgG titers. Therefore, co-detection of circulating antigens with IgM antibodies may improve the reliability of the diagnosis of acute toxoplasmosis.
Abstract
In this experiment, the correlation between antigenemia and specific antibody responses in Toxoplasma gondiiinfected rabbits was assessed. We injected 1,000 T. gondii tachyzoites (RH) subcutaneously into 5 rabbits. Parasitemia, circulating antigens, and IgM and IgG antibody titers in blood were tested by ELISA and immunoblot. For detection of parasitemia, mice were injected with blood from rabbits infected with T. gondii and mice died between days 2 and 10 post-infection (PI). Circulating antigens were detected early on day 2 PI, and the titers increased from day 4 PI and peaked on day 12 PI. Anti-Toxoplasma IgM antibody titers increased on day 6 PI and peaked on days 14-16 PI. IgG was detected from day 10 PI, and the titers increased continuously during the experiment. The antigenic protein patterns differed during the infection period, and the number of bands increased with ongoing infection by the immunoblot analysis. These result indicated that Toxoplasma circulating antigens during acute toxoplasmosis are closely related to the presence of parasites in blood. Also, the circulating antigen levels were closely correlated with IgM titers, but not with IgG titers. Therefore, co-detection of circulating antigens with IgM antibodies may improve the reliability of the diagnosis of acute toxoplasmosis.
참고문헌 (14)
- Ajioka JW, "Toxoplasma: Molecular and Cellular Biology", pp.37~58 (2007), Horizon Bioscience
- Shaapan RM, "Sensitivity and specificity of various serological tests for the detection of Toxoplasma gondii infection in naturally infected sheep", Vet Parasitol, 153, pp.359~362 (2008)
- Yokota M, "Effects of heparin on polymerase chain reaction for blood white cells", J Clin Lab Anal, 13, pp.133~140 (1999)
- Voller A, "Enzyme immunoassays with special reference to ELISA techniques", J Clin Pathol, 31, pp.507~520 (1978)
- Chen R, "Evaluation of a rapid ELISA technique for detection of circulating antigens of Toxoplasma gondii", Microbiol Immunol, 52, pp.180~187 (2008)
- Hafid J, "Detection of circulating antigens of Toxoplasma gondii in human infection", Am J Trop Med Hyg, 52, pp.336~339 (1995)
- Hassan MM, "The importance of detecting circulating Toxoplasma antigens in human cases", J Egypt Soc Parasitol, 27, pp.27~34 (1997)
- Susanto L, "Preparation of Toxoplasma gondii RH strain antigen, antigen analysis and antigen detection in sera: a review", Southeast Asian J Trop Med Public Health, 32(suppl 2), pp.195~201 (2001)
- Ise Y, "Detection of circulating antigens in sera of rabbits infected with Toxoplasma gondii", Infect Immun, 48, pp.269~272 (1985)
- Acebes MV, "Detection of circulating antigens in the diagnosis of acute toxoplasmosis", Am J Trop Med Hyg, 51, pp.506~511 (1994)
- Charde$s T, "Antibody responses to Toxoplasma gondii in sera, intestinal secretions, and milk from orally infected mice and characterization of target antigens", Infect Immun, 58, pp.1240~1246 (1990)
- Makioka A, "Recognition of tachyzoite and bradyzoite antigens of Toxoplasma gondii by infected hosts", Infect Immun, 59, pp.2763~2766 (1991)
- Cazabonne P, "Kinetics study and characterisation of target excreted", secreted antigens of immunoglobulin G, M, A and E antibodies from mice infected with different strains of Toxoplasma gondii, 1994, pp.80, 58~63
- Lee YH, "Detection of Toxoplasma antigens and antibodies in mice infected with different strains of Toxoplasma gondii", Korean J Parasitol, 33, pp.201~210 (1995)