Dirofilaria repens in Vietnam: Detection of 10 Eye and Subcutaneous Tissue Infection Cases Identified by Morphology and Molecular Methods
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.
Abstract
From 2006 to 2010, hospitals in Hanoi treated 10 human patients for dirofilariasis. The worms were collected from parasitic places, and identification of the species was completed by morphology and molecular methods. Ten parasites were recovered either from the conjunctiva (n=9) or subcutaneous tissue (n=1). The parasites were 4.0-12.5 cm in length and 0.5-0.6 mm in width. Morphological observations suggested all parasites as Dirofilaria repens. Three of the 10 parasites (1 from subcutaneous tissue and 2 from eyes) were used for molecular confirmation of the species identification. A portion of the mitochondrial cox1 (461 bp) was amplified and sequenced. Nucleotide and amino acid homologies were 95% and 99-100%, respectively, when compared with D. repens (Italian origin, GenBank AJ271614; DQ358814). This is the first report of eye dirofilariasis and the second report of subcutaneous tissue dirofilariasis due to D. repens in Vietnam.
참고문헌 (17)
- Pampiglione S, "Human dirofilariasis due to Dirofilaria (Nochtiella) repens: An update of world literature from 1995 to 2000", Parasitologia, 42, pp.231~254 (2000)
- Cancrini, G, "Aedes albopictus and Culex pipiens implicated as natural vectors of Dirofilaria repens in central Italy", J Med Entomol, 44, pp.1064~1066 (2007)
- Canestri TG, "The species of the genus Dirofilaria Railliet & Henry, 1911", Parasitology, 39, pp.369~374 (1997)
- Hoa LV, "Comparative study of Dirofilaria macacae, Sandground1933, a parasite of primates, and Dirofilaria repens, Raillet and Henry 1911, a parasite of Vietnamese carnivora", Bull Soc PatholExot Filiales, 64, pp.347~360 (1971)
- Dujic MP, "Orbital swelling as a sign of live Dirofilaria repens in subconjunctival tissue", Scand J Infect Dis, 35, pp.430~431 (2003)
- Raniel Y, "Subconjunctival infection with Dirofilaria repens", Isr Med Assoc J, 8, pp.139~ (2006)
- Rishniw M, "Discrimination between six species of canine microfilariae by a single polymerase chain reaction", Vet Parasitol, 135, pp.303~314 (2006)
- Casiraghi M, "A phylogenetic analysis of filarial nematodes: Comparison with the phylogeny of Wolbachia endosymbionts", Parasitology, 122, pp.93~103 (2011)
- Hu M, "Mitochondrial genomes of parasitic nematodes- progress and perspectives", Trends Parasitol, 22, pp.78~84 (2006)
- Le TH, "Mitochondrial genomes of parasitic flatworms", Trends Parasitol, 18, pp.206~213 (2002)
- Miyazaki I, "An Illustrated Book of Helminthic Zoonoses", pp.422~436 (1991), International Medical Foundation of Japan
- De NV, "Human dirofilariasis in the world and the first identification for species in Vietnam", J Pharmaceut Med, 5, pp.11~15 (2008)
- Dang TCT, "A human case of subcutaneous dirofilariasis caused by Dirofilaria repens in Vietnam: Histologic and molecular confirmation", Parasitol Res, 107, pp.1003~1007 (2010)
- Hu M, "Structure and organization of the mitochondrial genome of the canine heartworm, Dirofilaria immitis", Parasitology, 127, pp.37~51 (2003)
- Ghedin E, "Draft genome of the filarial nematode parasite Brugia malayi", Science, 317, pp.1756~1760 (2007)
- Keddie EM, "The mitochondrial genome of Onchocerca volvulus: sequence, structure and phylogenetic analysis", Mol Biochem Parasitol, 95, pp.111~127 (1998)
- Tamura K, "Prospects for inferring very large phylogenies by using the neighbor-joining method", Proc Natl Acad Sci USA, 101, pp.11030~11035 (2007)