Oxidative Stress in Vivax Malaria
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Malaria is still a leading cause of morbidity and mortality. The increase in lipid peroxidation reported in malaria infection and antioxidant status may be a useful marker of oxidative stress during malaria infection. The aim of this study was to investigate the role of antioxidant enzymes against toxic reactive oxygen species in patients infected with Plasmodium vivax and healthy controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were determined in 91 P. vivax patients and compared with 52 controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were 8.07±2.29 nM/ml, 2.69±0.33 U/ml, and 49.6±3.2 U/g Hb in the patient group and 2.72±0.50 nM/ml, 3.71±0.47 U/ml, and 62.3±4.3 U/g Hb in the control group, respectively. Malondialdehyde levels were found statistically significant in patients with vivax malaria higher than in healthy controls (P<0.001). On the other hand, superoxide dismutase and glutathione peroxidase activities were found to be significantly lower in vivax malaria patients than in controls (P<0.05). There was an increase in oxidative stress in vivax malaria. The results suggested that antioxidant defense mechanisms may play an important role in the pathogenesis of P. vivax.
Abstract
Malaria is still a leading cause of morbidity and mortality. The increase in lipid peroxidation reported in malaria infection and antioxidant status may be a useful marker of oxidative stress during malaria infection. The aim of this study was to investigate the role of antioxidant enzymes against toxic reactive oxygen species in patients infected with Plasmodium vivax and healthy controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were determined in 91 P. vivax patients and compared with 52 controls. Malondialdehyde levels, superoxide dismutase, and glutathione peroxidase activities were 8.07±2.29 nM/ml, 2.69±0.33 U/ml, and 49.6±3.2 U/g Hb in the patient group and 2.72±0.50 nM/ml, 3.71±0.47 U/ml, and 62.3±4.3 U/g Hb in the control group, respectively. Malondialdehyde levels were found statistically significant in patients with vivax malaria higher than in healthy controls (P<0.001). On the other hand, superoxide dismutase and glutathione peroxidase activities were found to be significantly lower in vivax malaria patients than in controls (P<0.05). There was an increase in oxidative stress in vivax malaria. The results suggested that antioxidant defense mechanisms may play an important role in the pathogenesis of P. vivax.
참고문헌 (25)
- Li J, "Geographic subdivision of the range of the malaria parasite Plasmodium vivax", Emerg Infect Dis, 7, pp.35~42 (2001)
- WHO Expert Committee on Malaria: Twentieth report, "World Health Organization", WHO, 3~18, pp.WHO Technical Report Series, No. 892 (1998)
- World Health Organization, "New Perspectives, Malaria Diagnosis", pp.5~40 (2000), WHO
- Akdur R, "Sıtma Temel Bilgiler", pp.1~21 (2004), Palme Yayıncılık
- Becker K, "Oxidative stress in malaria parasite-infected erythrocytes: host-parasite interactions", Int J Parasitol, 34, pp.163~189 (2004)
- Schwartz E, "The role of superoxide dismutation in malaria parasites", Inflammation, 23, pp.361~370 (1999)
- Mishra NC, "Oxidative stress and malaria-infected erythrocytes", Indian J Malariol, 31, pp.77~87 (1994)
- Sarin K, "Oxidative stress and antioxidant defence mechanism in Plasmodium vivax malaria before and after chloroquine treatment", Indian J Malariol, 30, pp.127~133 (1993)
- Draper HH, "A review of recent studies on the metabolism of exogenous and endogenous malondialdehyde", Xenobiotica, 20, pp.901~907 (1990)
- Nayak DU, "Antioxidant vitamins and enzymatic and synthetic oxygen-derived free radical scavengers in the prevention and treatment of cardiovascular disease", Heart Dis, 3, pp.28~45 (2001)
- Romero FJ, "Lipid peroxidation products and antioxidants in human disease", Environ Health Perspect, 106, pp.1229~1234 (1998)
- Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Anal Biochem, 95, pp.351~358 (1979)
- Sun Y, "A simple method for clinical assay of superoxide dismutase", Clin Chem, 34, pp.497~500 (1988)
- Beutler E, "Red Cell Metabolism: A Manual of Biochemical Methods", pp.74~76 (1975), Grune and Starton
- Descamps-Latscha B, "Generation of reactive oxygen species in whole blood from patients with acute falciparum malaria", Parasite Immunol, 9, pp.275~279 (1987)
- Kaikai P, "The influence of riboflavin deficiency on Plasmodium berghei infection in rats", Trans R Soc Trop Med Hyg, 77, pp.680~686 (1983)
- Thurnham DI, "Riboflavin status and malaria in infants in Papua New Guinea", Trans R Soc Trop Med Hyg, 77, pp.423~424 (1983)
- Erel O, "Oxidative stress and antioxidative status of plasma and erythrocytes in patients with vivax malaria", Clin Biochem, 30, pp.631~639 (1997)
- Blum J, "Inactivation of glutathione peroxidase by superoxide radical", Arch Biochem Biophys, 240, pp.500~508 (1985)
- Jacob HS, "Effects of sulfhydryl inhibition on red blood cells. 3. Glutathione in the regulation of the hexose monophosphate pathway", J Biol Chem, 241, pp.4243~4250 (1966)
- Bhattacharya J, "Reduction in erythrocytic GSH level and stability in Plasmodium vivax malaria", Trans R Soc Trop Med Hyg, 81, pp.64~66 (1987)
- Das BS, "Evidence for erythrocyte lipid peroxidation in acute falciparum malaria", Trans R Soc Trop Med Hyg, 93, pp.58~62 (1999)
- Yazar S, "Serum malondialdehyde levels in patients infected with Plasmodium vivax", West Indian Med J, 53, pp.147~149 (2004)
- Pabón A, "Oxidative stress in patients with non-complicated malaria", Clin Biochem, 36, pp.71~78 (2003)
- Hamel CT, "Nonoperative treatment of splenic rupture in malaria tropica: review of literature and case report", Acta Trop, 82, pp.1~5 (2002)