The Route of Leishmania tropica Infection Determines Disease Outcome and Protection against Leishmania major in BALB/c Mice
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Leishmania tropica is one of the causative agents of leishmaniasis in humans. Routes of infection have been reported to be an important variable for some species of Leishmania parasites. The role of this variable is not clear for L. tropica infection. The aim of this study was to explore the effects of route of L. tropica infection on the disease outcome and immunologic parameters in BALB/c mice. Two routes were used; subcutaneous in the footpad and intradermal in the ear. Mice were challenged by Leishmani major, after establishment of the L. tropica infection, to evaluate the level of protective immunity. Immune responses were assayed at week 1 and week 4 after challenge. The subcutaneous route in the footpad in comparison to the intradermal route in the ear induced significantly more protective immunity against L. major challenge, including higher delayed-type hypersensitivity responses, more rapid lesion resolution, lower parasite loads, and lower levels of IL-10. Our data showed that the route of infection in BALB/c model of L. tropica infection is an important variable and should be considered in developing an appropriate experimental model for L. tropica infections.
Abstract
Leishmania tropica is one of the causative agents of leishmaniasis in humans. Routes of infection have been reported to be an important variable for some species of Leishmania parasites. The role of this variable is not clear for L. tropica infection. The aim of this study was to explore the effects of route of L. tropica infection on the disease outcome and immunologic parameters in BALB/c mice. Two routes were used; subcutaneous in the footpad and intradermal in the ear. Mice were challenged by Leishmani major, after establishment of the L. tropica infection, to evaluate the level of protective immunity. Immune responses were assayed at week 1 and week 4 after challenge. The subcutaneous route in the footpad in comparison to the intradermal route in the ear induced significantly more protective immunity against L. major challenge, including higher delayed-type hypersensitivity responses, more rapid lesion resolution, lower parasite loads, and lower levels of IL-10. Our data showed that the route of infection in BALB/c model of L. tropica infection is an important variable and should be considered in developing an appropriate experimental model for L. tropica infections.
참고문헌 (22)
- Control of the Leishmaniases: Report of a Meeting of the WHO Expert Committee on the Control of Leishmaniases, "World Health Organization", WHO, ~, pp.WHO Technical Report Series, No. 949 (2010)
- Hamid Mahmoudzadeh-Niknam, "Viscerotropic growth pattern of Leishmania tropica in BALB", c mice is suggestive of a murine model for human viscerotropic leishmaniasis, 2007, pp.45(4), 247~253[DOI]
- Lira R, "Leishmania tropica: the identification and purification of metacyclic promastigotes and use in establishing mouse and hamster models of cutaneous and visceral disease", Exp Parasitol, 89, pp.331~342 (1998)
- Sacks D, "The immunology of susceptibility and resistance to Leishmania major in mice", Nat Rev Immunol, 2, pp.845~858 (2002)
- Hamid Mahmoudzadeh-Niknam, "Leishmania tropica infection, in comparison toLeishmania major, induces lower delayed type hypersensitivityin BALB", c mice, 2007, pp.45(2), 103~109[DOI]
- Constant SL, "Site of antigen delivery can influence T-cell priming: pulmonary environment promotes preferential Th2-type differentiation", Eur J Immunol, 30, pp.840~847 (2000)
- Méndez S, "Optimization of DNA vaccination against cutaneous leishmaniasis", Vaccine, 20, pp.3702~3708 (2002)
- Bhowmick S, "Vaccination route that induces transforming growth factor beta production fails to elicit protective immunity against Leishmania donovani infection", Infect Immun, 77, pp.1514~1523 (2009)
- Tabbara KS, "Conditions influencing the efficacy of vaccination with live organisms against Leishmania major infection", Infect Immun, 73, pp.4714~4722 (2005)
- Belkaid Y, "Development of a natural model of cutaneous leishmaniasis: powerful effects of vector saliva and saliva preexposure on the long-term outcome of Leishmania major infection in the mouse ear dermis", J Exp Med, 188, pp.1941~1953 (1998)
- Kaur S, "Effect of dose and route of inoculation on the generation of CD4+ Th1", Th2 type of immune response in murine visceral leishmaniasis, 2008, pp.103, 1413~1419
- Mahmoudzadeh-Niknam H, "The problem of mixing up of Leishmania isolates in the laboratory: suggestion of ITS1 gene sequencing for verification of species", Iran J Parasitol, 6, pp.41~48 (2011)
- Mahmoudzadeh-Niknam H, "Protective immunity against Leishmania major induced by Leishmania tropica infection of BALB", c mice, 2011, pp.127, 448~453
- Mahmoudzadeh-Niknam H, "Induction of partial protection against Leishmania major in BALB", c mice by Leishmania tropica, 2004, pp.31, 201~207
- Mohanan D, "Administration routes affect the quality of immune responses: A cross-sectional evaluation of particulate antigen-delivery systems", J Control Release, 147, pp.342~349 (2010)
- Cubas R, "Virus-like particle (VLP) lymphatic trafficking and immune response generation after immunization by different routes", J Immunother, 32, pp.118~128 (2009)
- Sacks DL, "Animal models for the analysis of immune responses to leishmaniasis, In Current Protocols in Immunology", pp.19.2.1~19.2.20. (1998), John Wiley & Sons
- Combadiere B, "Transcutaneous and intradermal vaccination", Hum Vaccin, 7, pp.811~827 (2011)
- Kautz-Neu K, "Langerhans cells are negative regulators of the anti-Leishmania response", J Exp Med, 208, pp.885~891 (2011)
- Bennett CL, "Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity", J Cell Biol, 169, pp.569~576 (2005)
- Kirkpatrick CE, "Rate of Leishmania-induced skin-lesion development in rodents depends on the site of inoculation", Parasitology, 94, pp.451~465 (1987)
- Baldwin TM, "The Site of Leishmania major infection determines disease severity and immune responses", Infect Immun, 71, pp.6830~6834 (2003)