Complete Mitochondrial Genome of Haplorchis taichui and Comparative Analysis with Other Trematodes
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Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.
Abstract
Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.
참고문헌 (29)
- Chai JY, "Fish-borne parasitic zoonoses : status and issues", Int J Parasitol, 35, pp.1233~1254 (2005)
- Dung DT, "Fishborne zoonotic intestinal trematodes, Vietnam", Emerg Infect Dis, 13, pp.1828~1833 (2007)
- 채종일, "Foodborne Intestinal Flukes in Southeast Asia", The Korean Journal of Parasitology, 47, pp.69~102 (2009), 대한기생충학ㆍ열대의학회
- Thaenkham U, "Discrimination of Opisthorchis viverrini from Haplorchis taichui using COI sequence marker", Acta Trop, 103, pp.26~32 (2007)
- 전형규, "Human Infections with Liver and Minute Intestinal Flukes in Guangxi, China: Analysis by DNA Sequencing, Ultrasonography, and Immunoaffinity Chromatography", The Korean Journal of Parasitology, 50(4), pp.391~394 (2012), 대한기생충학ㆍ열대의학회
- 채종일, "Foodborne Trematode Metacercariae in Fish from Northern Vietnam and Their Adults Recovered from Experimental Hamsters", The Korean Journal of Parasitology, 50(4), pp.317~325 (2012), 대한기생충학ㆍ열대의학회
- Wongsawad C, "Coprodiagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods", Southeast Asian J Trop Med Public Health, 40, pp.924~928 (2009)
- Jex AR, "The mitochondrial genome of Toxocara canis", PLos Negl Trop Dis, 2, pp.e273~ (2008)
- Lawton SP, "Genomes and geography : genomic insights into the evolution and phylogeography of the genus Schistosoma", Parasit Vectors, 4, pp.131~ (2011)
- Cavalier-Smith T, "Protist phylogeny and the high-level classification of Protozoa", Eur J Protistol, 39, pp.338~348 (2003)
- Gissi C, "Evolution of the mitochondrial genome of Metazoa as exemplified by comparison of congeneric species", Heredity, 101, pp.301~320 (2008)
- Jeon HK, "Complete sequence of the mitochondrial genome of Taenia saginata: comparison with T. solium and T. asiatica", Parasitol Int, 56, pp.243~246 (2007)
- Park JK, "Characterization of the mitochondrial genome of Diphyllobothrium latum(Cestoda : Pseudophyllidea)-implications for the phylogeny of eucestodes", Parasitology, 134, pp.749~762 (2007)
- Liu GH, "Comparative analyses of the complete mitochondrial genomes of Ascaris lumbricoides and Ascaris suum from humans and pigs", Gene, 492, pp.110~116 (2012)
- Shekhovsov SV, "The complete mitochondrial genomes of the liver flukes Opisthorchis felineus and Clonorchis sinensis(Trematoda)", Parasitol Int, 59, pp.100~103 (2010)
- Kyu-Heon Kim, "Characterization of the Complete Mitochondrial Genome of Diphyllobothrium nihonkaiense (Diphyllobothriidae: Cestoda), and Development of Molecular Markers for Differentiating Fish Tapeworms", Molecules and Cells, 23(3), pp.379~390 (2007), 한국분자세포생물학회
- Cai XQ, "Sequences and gene organization of the mithochondrial genomes of the liver flukes Opisthorchis viverrini and Clonorchis sinensis(Trematoda)", Parasitol Res, 110, pp.235~243 (2012)
- Le TH, "Mitochondrial genomes of parasitic flatworms", Trends Parasitol, 18, pp.206~213 (2002)
- Olson PD, "Phylogeny and classification of Digenea(Platyhelminthes : Trematoda)", Int J Parasitol, 33, pp.733~755 (2003)
- Kearse M, "Geneious Basic : an integrated and extendable desktop software platform for the organization and analysis of sequence data", Bioinfomatics, 28, pp.1647~1649 (2012)
- Talavera G, "Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments", Syst Biol, 56, pp.564~577 (2007)
- Masters BC, "Species delimitation-a geneious plugin for the species boundaries", Mol Ecol Resour, 11, pp.154~157 (2010)
- Swofford DL, "Paup*: phylogenetic analysis using parsimony (* and other methods). Version 4" (2003), Sinauer Associates
- Guindon S, "A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood", Syst Biol, 52, pp.696~704 (2003)
- Huelsenbeck JP, "MRBAYES : Bayesian inference of phylogeny", Bioinformatics, 17, pp.754~755 (2001)
- Johnston DA, "Parasitic Flatworms: Molecular Biology, Biochemistry, Immunology and Physiology", pp.37~80 (2006), CAB International
- Justine JL, "Phylogeny of parasitic Platyhelminthes : a critical study of synapomorphies proposed on the basis of the ultrastructure of spermiogenesis and spermatozoa", Can J Zool, 69, pp.1421~1440 (1991)
- 전형규, "Immunoblot Patterns of Taenia asiatica Taeniasis", The Korean Journal of Parasitology, 47(1), pp.73~77 (2009), 대한기생충학ㆍ열대의학회
- Thaenkham U, "Families Opisthorchiidae and Heterophyidae : are they distinct?", Parasitol Int, 61, pp.90~93 (2012)