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한국여성의 질에서 분리된 유산간균총의 미생물학적, 분자유전학적 분석 및 우량간균 KLB46을 이용한질염 치료의 예비적 임상실험 결과

Microbiologic and Molecular Genetic Analysis of Lactobacillus spp.Isolated from Vagina of Korean Women and A Pilot Clinical Studyon the Treatment of Vaginitis using the Best

박미영|박한뫼|김승철|소재성
대한산부인과학회 (Korean Soceity of Obstetrics and Gynecology)|2004.06|Obstetrics & Gynecology Science|제47권 제6호|pp. 1154-1164

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참고문헌

키워드

ProbioticsBacterial vaginosis세균성 질증Lactobacillus crispatus KLB 46
주제분류산부인과학

초록

목적 : 한국 여성의 질에서 유산간균총을 분리하여 그 중 probiotics로서 사용될 수 있는 유력한 유산간균주를 선정하여 질염의 치료효과를 확인하고자 하였다. 연구 방법 : 205명의 건강한 여자의 질 분비물로부터 분리된 유산균주들을 catalase test, 세포 표면 소수성 검사, 항균 활성도 측정, Restriction fragment length polymorphism (RFLP)을 이용한 분자 유전학적 분석 등을 시행하여 그 종류와 분포 및 항생제 감수성을 분석하였다. 또한 L. crispatus KLB 46 균주를 이용하여 세균성 질증 및 위축성 질염 환자를 대상으로 예비적 임상실험을 시행하였다. 결과 : 분리된 유산간균주는 105종으로 대부분 항균활성을 보였다. 가장 많이 분포하는 우점균주는 L. crispatus와 L. acidophilus (51%)였고, 우량균주 KLB 46은 L. crispatus 였다. L. crispatus KLB 46을 질 내에 접종한 후 세균성 질증 환자와 위축성 질염 환자에서 우수한 치료효과를 보였다. 결론 : 한국 여성의 질에서 분리된 유산간균총의 우점균주는 성장속도, 생존력, 항균활성에서 모두 좋은 결과를 보이며, L. crispatus KLB 46은 세균성 질증의 예비적 임상실험에서 우수한 치료결과를 보여 향후 probiotics로서 유망한 균주로 사료된다.

Abstract

Objective : The aim of this study was to analize the biologic and molecular genetic characteristics of Lactobacillus spp. isolated from vagina of Korean healthy women and to perform a pilot clinical study on the treatment of vaginitis using the best Lactobacillus strain KLB 46. Methods : Vaginal specimens were obtained from 205 Korean women with healthy vaginal ecosystems. 155 lactobacillus strains were used for catalase test, cell surface hydrophobicity (CSH) test and antimicrobial activity test. Also we identified the species prevalence by PCR-RFLP analysis of 16S rDNA gene and performed the minimal inhibitory concentrationtest using14 antibiotics to determine their patterns of antibiotic susceptibility. A pilot clinical trial of 8 bacterial vaginosis, 1 trichomonas vaginitis and 6 atrophic vaginitis using Lactobacillus crispatus KLB 46 was done by vaginal instillation (1010-1011 cells/mL). Results : 155 lactobacilli strains were isolated and all isolates showed high CSH and 61% of the isolates had higher CSH. L. acidophilus and L. crispatus were the most prevalent species by PCR-RFLP analysis of their 16S rDNA gene. And PCR-RFLP analysis suggested that KLB 46 was L. crispatus. The clinical study showed that the vaginal epithelial adherence rate was high as 77% for bacterial vaginosis and 33.3% for atrophic vaginitis. Conclusion : The predominating lactobacilli were L. crispatus and L. acidophilus in Korean women's vaginas. Although further study would be needed, L. crispatus KLB 46 is a good candidate of suitable probiotics for bacterial vaginosis.

참고문헌 (42)

  1. "Establishing the cause of genitourinary symptoms in women in a family practice. Comparison of clinical examination and comprehensive microbiology", 3(251), pp.620~625 (1984)
  2. "Bacterial vaginosis. In:Sexually transmitted diseases", pp.547~559 (1990), McGraw-Hill
  3. "Bacterial vaginosis and women and babies", 67(1), pp.S9~S11 (1999)
  4. "Efficacy of intravaginal 0 75% metronidazole gel for the treatment of bacterial vaginosis", 81(6), pp.963~967 (1993)
  5. "Potential role of lactobacilli as prophylactic agents against genital pathogens", 13(12), pp.747~751 (1999)
  6. "Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women and women with bacterial vaginosis", 27(2), pp.251~256 (1989)
  7. "Evaluation of health and nutritional properties of powder milk and live lactic acid bacteria Food and Agriculture Organization of the United Nations and World Health Organization Report" (2001), http:
  8. "Characterisation and selection of a Lactobacillus species to recolonise the vagina of women with recurrent bacterial vaginosis", 49(6), pp.543~552 (2000)
  9. "Characterization and selection of vaginal Lactobacillus strains for the preparation of vaginal tablets", 93(5), pp.884~893 (2002)
  10. "Use of Lactobacillus to prevent infection by pathogenic bacteria", 4(3), pp.319~324 (2002)
  11. "Phage infection in vaginal lactobacilli:An in vitro study", 5, pp.36~44 (1997)
  12. "Analysis of Lactobacillus phage and bacteriocins in American dairy products and characterization of a phage isolated fromyogurt", 62, pp.2111~2116 (1996)
  13. "Increased cell surfacehydrophobicity of a lipopolysaccharide-defective mutant of Bradyrhzobium Japonicum", 5, pp.241~243 (1995)
  14. "Plasmid-associated bacteriocin production by a strain of Carnobacterium piscicola from meat", 56, pp.2503~2510 (1990)
  15. "Lacticin 3127, a broad-spectrum bacteriocin which selectively dissipates the membrane potential", 64, pp.439~445 (1998)
  16. "Properties of the Fusants of Lactobacillus acidophilus 88and Lactobacillus casei subsp casei KCTC 1121", 7, pp.25~31 (1997)
  17. "Plasmid-associated bacteriocin production by Leuconostoc sp. LAB145-3A Isolatedfrom Kimchi", 7, pp.409~416 (1997)
  18. "Effect of pH and hydrogen peroxide produced by Lactobacillus hilgardii onPediococcus pentosaceus growth", 128, pp.59~62 (1995)
  19. "Molecular identification of vaginal lactobacillus spp isolated from Korean women", 12, pp.312~317 (2002)
  20. "16S ribosomal DNA amplification for phylogenetic study", 173(2), pp.697~703 (1991)
  21. "Fermentation optimization of Plantaricin 423, a bacteriocin produced by Lactobacillus plantarum 423", 86, pp.174~179 (1998)
  22. "Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora", 12(5), pp.856~872 (1990)
  23. "Genetic diversity of vaginal lactobacilli from women in different countries based on 16S rRNA gene sequences", 92(3), pp.451~459 (2002)
  24. "The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species", 180(6), pp.1950~1956 (1999)
  25. "Identification of vaginal lactobacilli from asymptomatic women", 10(4), pp.377~384 (1987)
  26. "Taxonomy of the Lactobacillus acidophilus group", 30, pp.53~68 (1980)
  27. "Evaluation of random amplified polymorphic DNA(RAPD)-PCR as a method to differentiate Lactobacillus acidophilus, Lactobacillus crispatus, Lactobacillus amylovorus, Lactobacillus gallinarum, Lactobacillus gasseri, and Lactobacillus johnsonii", 31(2), pp.114~118 (1995)
  28. "Identification of and hydrogen peroxide production by fecal and vaginal lactobacilli isolated from Japanese women and newborn infants", 37(9), pp.3062~3064 (1999)
  29. "2002 sexually transmitted diseases treatment guidelines", 51(6), pp.42~47 (2002)
  30. "Preterm labor associated with subclinical amniotic fluid infection and with bacterial vaginosis", 67, pp.229~237 (1986)
  31. "Association between bacterial vaginosis and preterm delivery of a low-birth-weight infant", 333, pp.1737~1742 (1995)
  32. "Bacterial vaginosis in early pregnancy and pregnancy outcome", 80, pp.173~177 (1992)
  33. "Gynecologic sequelae of bacterial vaginosis", 67(1), pp.S25~S28 (1999)
  34. "Metronidazole to prevent preterm delivery in pregnant women with asymptomatic bacterial vaginosis", 342(348), pp.534~540 (2000)
  35. "Impact of metronidazole therapy on preterm birth in women with bacterial vaginosis flora(Gardnerella vaginalis) a randomised, placebo controlled trial", 104(12), pp.1391~1397 (1997)
  36. "A longitudinal study of bacterial vaginosis during pregnancy", 101(12), pp.1048~1053 (1994)
  37. "Assessment of the capacity of Lactobacillus to inhibit the growth of uropathogens and block their adhesion to vaginal epithelial cells", 183(3), pp.485~491 (2001)
  38. "Therapy of bacterial vaginosis using exogenously-applied Lactobacillus acidophilus and a low dose of estriol", 46, pp.68~73 (1996)
  39. "Vaginitis", 337(26), pp.1896~1903 (1997)
  40. "Effect of metronidazole on the growth of vaginal lactobacilli in vitro", 9(1), pp.41~45 (2001)
  41. "Nitroimidazole drugs--action and resistance mechanisms. I. Mechanisms of action", 31(1), pp.9~20 (1993)
  42. "Growth inhibition of metronidazole- susceptible and metronidazole-resistant strains of Gardnerella vaginalis by Lactobacilli in vitro", 62(3), pp.1089~1092 (1996)

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