임신부에서 분리된 B군 연구균의 중합효소연쇄반응과 염기서열분석을 통한 혈청형 분석
Molecular Serotyping of Group B Streptococcus Isolated from the Pregnant Women by Polymerase Chain Reaction and Sequence Analysis
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목 적: 국내 임신부의 생식기와 직장에서 분리된 B군 연구 균의 혈청형 분포를 분자생물학적인 방법으로 확인하고자 하 였다. 방 법: 산전 진찰을 위해 고양시 소재 4개의 산부인과병원 을 방문한 임신 35주 이상 임신부의 생식기와 직장에서 분리 되어 -70℃에 보관 중이던 42개의 B군 연구균에서 DNA를 추출하고 혈청형 특이 PCR 과 염기서열 분석을 이용하여 혈청 형을 결정하였다. 결 과: 42개 균주 모두에서 혈청형 특이 PCR 과 염기서열 분석을 통해 혈청형을 결정할 수 있었고 빈도순으로 III형이 12균주(28.6%), V형이 11균주(26.2%), Ia형이 11균주(26.2 %), VI형이 4균주(9.5%), Ib형이 2균주(4.8%), II형이 2균주 (4.8%)였다. 결 론: 국내의 후기 임신부에서 흔하게 분리되는 B군 연 구균의 혈청형은 III형, V형, Ia형이었고 본 연구에서 이용 한 혈청형 특이 PCR과 염기서열 분석은 앞으로도 관련 연 구에 유용하게 사용할 수 있을 것으로 보인다.
Abstract
Purpose : This study was performed to investigate the serotype distribution of group B streptococcus (GBS) isolated from pregnant Korean women using molecular methods. Methods : The study materials included 42 GBS isolates obtained from the vagina and anorectum of pregnant women in Goyang, Korea between 2005 and 2006. Four clinical isolates with known serotypes (Ia, Ib, III, and V) were used for validation of molecular serotyping. We used serotype-specific primers for identification of the serotypes (Ia, Ib, III, V, and VI). To determine the ambiguous serotypes by serotype-specific PCR, sequence analysis of the PCR amplicons which had been amplified with GBS-common primers was used. Results : The serotypes determined by the molecular methods agreed with the previously known 4 serotypes (Ia, Ib, III, and V). The serotypes of all 42 isolates were successfully determined by molecular methods. The distribution of the GBS serotype was as follows in order of frequency: serotype III was found in 12 isolates (28.6%), serotype V was found in 11 isolates (26.2%), serotype Ia was found in 11 isolates (26.2%), serotype VI was found in 4 isolates (9.5%), serotype Ib was found in 2 isolates (4.8%), and serotype II was found in 2 isolates (4.8%). Conclusion : Serotypes III, V, and Ia were the most frequently identified serotypes in pregnant Korean women. Molecular serotyping is useful for surveillance of the serotype distribution of GBS in colonized pregnant women and GBS diseases of neonates.
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