과배란유도에서 난포액내 NO 농도와 난소반응 및연령과의 상관관계
Correlation of Nitric Oxide Concentrations in Follicular Fluidwith the Ovarian Response as well as the Age in Women Undergoing Controlled Ovarian Hyperstimulation
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
=목적: 체외수정을 위한 과배란유도를 시행 받는 여성에서 난포액내 NO 농도와 난소반응 및 연령과의 상관관계를 알아보고자 하였다. 연구 방법: 창원 한마음병원 및 부산대학교병원 불임클리닉에서 2003년 8월부터 12월까지 체외수정술을 받은 72주기 (72명)의 환자를 대상으로 하였다. 난자 채취 시 난포의 크기별로 구분하여 난포액을 회수한 후 난포액내 NO 농도를 측정하였다. 체외수정을 위한 과배란유도를 시행 받는 여성에서 난포액내 NO 농도와 난소반응 및 연령과의 상관관계를 분석하였다.결과: 72명 가운데 27명에서 임신되어 37.5%의 임신율을 보였다. 임신군과 비임신군에서 대상자의 연령, 사용된 성선자극호르몬 용량, hCG 투여당일 E2 농도, 회수된 난자수, 수정률 등은 유의한 차이가 없었다. 그러나 난포의 크기와 난포액내 NO 농도사이에는 음성적 상관관계를 보였다. 난포액내 NO 농도와 대상자의 연령사이에는 유의한 상관관계가 없었다. 대상자의 연령층 별로 임신군과 비임신군에서 난포액내 NO 농도를 비교한 결과, 난포액내 NO 농도는 40세 이상의 비임신군에서 임신군에 비해 유의하게 높았다.결론: 이러한 결과는 난포액내 NO 농도는 체외수정을 위한 과배란유도에서 난소반응과 상관관계가 없으며, 난포의 크기를 제외한 대상자 연령 및 모든 변수들과도 상관관계가 없다는 것을 시사한다.
Abstract
Objective: To elucidate the correlation of nitric oxide (NO) concentrations in follicular fluid (FF) with the ovarian response as well as the age in women undergoing controlled ovarian hyperstimulation (COH). Methods: This study was performed in 72 cycles of whom underwent COH for in vitro fertilization at infertility clinic of Changwon Hanmaeum Hospital and Pusan National University Hospital from Aug., 2003 to Dec., 2003. Each FF was collected at the oocyte retrieval and NO concentrations in FF were assessed. We analyzed the correlation of NO concentrations in FF with the ovarian response as well as the age in women undergoing COH. Results: Among 72 cycles, the clinical pregnancy rate was 37.5% (27cycles). There were no significant differences in patient’s age, ampules of gonadotropins used, estradiol level on the day of hCG administration, the number of oocytes retrieved, and fertilization rate between pregnant and nonpregnant groups. However, negative correlation was found between the follicular size and NO concentrations in FF. No significant correlation was found between age and NO concentrations in FF. Comparing NO concentrations in FF between pregnant and nonpregnant women by age, NO concentrations in FF were higher at nonpregnant women, significantly those of them more than 40 years old. Conclusion: These results suggest that NO concentrations in FF cannot be a marker for the ovarian response, and have no correlation with all parameters of COH including patients’ age with exception for the follicular size.
참고문헌 (24)
- Lee KS, "Relationships between concentrations of tumor necrosis factor-α and nitric oxide in follicular fluid and oocyte quality", 17, pp.222~7 (2000)
- Rosselli M, "Role of nitric oxide in the biology and pathophysiology of reproduction", 4, pp.3~24 (1998)
- Murohara T, "Vascular endothelial growth factor", vascular permeability factor enhances vascular permeability via nitric oxide and prostacyclin, 1998, pp.97, 99~107
- Hum Reprod 2000, "Follicular fluid concentrations of adrenomedullin vascular endothelial growth factor and nitric oxide in IVF cycles relationship to ovarian response", 15, pp.1295~9 (2000)
- Sugino N, "Nitric oxide concentrations in the follicular fluid and apoptosis of granulosa cells in human follicles", 11, pp.2484~7 (1996)
- Van der Zee R, "augments nitric oxide release from quiescent rabbit and human vascular endothelium", 95, pp.1030~1037 (1997)
- Kubes P, "Nitric oxide modulates microvascular permeability", 262, pp.~5 (1992)
- Barroso G, "and leptin follicular fluid levels correlate negatively with embryo quality in IVF patients", 72, pp.1024~6 (1999)
- Gordon JD, "Angiogenesis in the human female reproductive tract", 50, pp.688~97 (1995)
- Friedman CI, "Follicular fluid vascular endothelial growth factor concentrations are elevated in women of advanced reproductive age undergoing ovulation induction", 68, pp.607~12 (1997)
- Ravindranath L, "Vascular endothelial growth factor messenger ribonucleic acid expression in the primate ovary", 131, pp.254~60 (1992)
- Kamat BR, "Expression of vascular permeability factor", vascular endothelial growth factor by human granulosa and theca lutein cells:Role in corpus luteum development, 1995, pp.146, 157~65
- Nguyen LS, "Substance P increases microvascular permeability via nitric oxide-mediated convective pathways", 268, pp.~8 (1995)
- Barrionuevo MJ, "Follicular fluid nitric oxide level and its impact on oocyte potential", 7, pp.165~7 (1997)
- Revel A, "Characterization of intraperitoneal cytokines and nitrites in women with severe ovarian hyperstimulation syndrome", 66, pp.66~71 (1996)
- Griess LC, "Analysis of nitrate nitrite and 15N nitrate in biological fluid", wish(k js), pp.131~8
- Bourne TH, "Ultrasound studies of vascular and morphological changes in the human corpus luteum during the menstrual cycle", 65, pp.753~8 (1996)
- Steer CV, "The use of transvaginal color flow imaging after in vitro fertilization to identify optimum uterine conditions before embryo transfer", 57, pp.372~6 (1992)
- Van Blerkom J, "The developmental potential of the human oocyte is related to the dissolved oxygen content of follicular fluid:association with vascular endothelial growth factor levels and perifollicular blood flow characteristics", 12, pp.1047~55 (1997)
- Van Blerkom J, "Epigenetic influences on oocyte developmental competence:perifollicular vascularity and intrafollicular oxygen", 15, pp.226~34 (1998)
- Jinno M, "Measurement of endometrial tissue blood flow:a novel way to assess uterine receptivity for implantation", 76, pp.1168~74 (2001)
- Powers RW, "Gonadotropin-stimulated regulation of blood-follicle barrier is mediated by nitric oxide", 269, pp.~8 (1995)
- Faller DV, "Endothelial cell responses to hypoxic stress", 26, pp.74~84 (1999)
- Voorhis BJ, "Nitric oxide:An autocrine regulator of human granulosa-luteal cell steroidogenesis", 135, pp.1799~806 (1994)