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제대혈 유래 중간엽 줄기세포의 분리 및 특성 규명

Isolation and characterization of mesenchymal stem cells derived from umbilical cord blood

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주제분류산부인과학

초록

=목적: 제대혈 내 중간엽 줄기세포의 분리법을 개발하고 특성을 규명하고자 하였다. 연구 방법: 제대혈로부터 중간엽 줄기세포를 분리하여 제대혈 중간엽 줄기세포의 증식력, 세포주기, 세포화학 지침자, 면역표현형의 발현도를 측정하였고 골아세포로의 분화도와 지방세포로의 분화도를 조사 분석하였다.결과: 제대혈로부터 분리된 중간엽 줄기세포의 84% 이상이 세포주기 G0/G1기에 있었고 α-naphthyl acetate esterase, periodic acid-Schiff에 양성반응을 보였으나 sudan black-B, ALP에 음성반응을 보였다. 이들 줄기세포는 CD13, CD29, CD44, CD49e, CD51, CD54, CD90, SH2, SH3 항원을 발현하였으나 CD11b, CD14, CD31, CD34, CD45, CD49d, CD106, CD133, CD144, CD146, CD163, CD166, Stro-1 항원은 발현하지 않았다. 골생성 배양액에서 배양 시 골아세포의 지침자인 ALP, Runx-2, 제1형 procollagen의 mRNA가 발현되었고 칼슘의 축적 및 ALP활성이 관찰되었다. 지방세포 형성 배양액에서 배양 후 지질축적이 관찰되었다.

참고문헌 (30)

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