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Cisplatin 내성 난소암 세포주로부터 2차 내성 아세포 수립과 성격규명

Establishment and characterization of secondary drug resistant cell line originating from cisplatin-resistant SNU-8

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키워드

주제분류산부인과학

초록

=목적: 난소암에서 1차 항암치료로 백금제제를 사용한 저항성 재발 난소암 환자는 2차 항암제에 반응율이 낮고 또한 재발율도 높다. 본 논문에서는 2차 항암제에 대한 내성 난소암 아세포주를 수립하여 항암제 내성기전을 규명고자 하였다.방법: 2차 항암제 내성 아세포주 SNU-8/Fac를 수립하여 MTT assay로 항암제 감수성을 측정하였고 RT-PCR방법과 western blot방법으로 내성 기전을 규명하였다. 결과: 2차 항암제 내성 아세포주 SNU-8/Fac를 수립하여 항암제 감수성을 측정한 결과 multidrug resistance를 보였다. 내성 관련 유전자를 관찰한 결과 mRNA수준에서는 LRP는 발현 정도를 관찰할 수 없었고 MRP1, BCRP, TS와 MT는 SNU-8/Fac에서 SNU-8/WT보다 더 과발현되지 않았으나 MDR1와 ATP7B는 더 과발현되었고 protein수준에서는 P-gp와 ATP7B가 더 과발현되었다.

참고문헌 (38)

  1. "난소암 조직에서 HLA-G유전자 발현과 예후 인자와의 상관성", 48(12), pp.2910~2918 (2005)
  2. "Remaining controversies in the upfront management of advanced ovarian cancer", 14(5), pp.707~720 (2004)
  3. "Chemotherapy of advanced ovarian cancer", 25(3), pp.340~348 (1998)
  4. "Update in the management of advanced ovarian carcinoma", 32, pp.49~58 (1999)
  5. "Preservation of the recipient inferior vena cava in liver transplantation", 1, pp.~1 (1994)
  6. "Internal duplication and homology with bacterial transport proteins in the mdr1 gene from multidrug-resistance human cells", pp.381~9
  7. "Overexpression of a transporter gene in a multidrug resistant human lung cancer cell line", pp.1650~4
  8. "Transport of glutathione conjugates and glucuronides by the multidrug resistance proteins MRP1 and MRP2", 378, pp.787~791 (1997)
  9. "Drug resistance-associated marker Lrp for prediction of response to chemotherapy and prognosis in advanced ovarian carcinoma", 87, pp.1230~7 (1985)
  10. "A multidrug resistance transporter from human MCF-7 breast cancer cells", 95(26), pp.15665~15670 (1998)
  11. "Surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants", 455, pp.152~62 (1976)
  12. "Amplification of P-glycoprotein genes in multidrug-resistant mammalian cell lines", 316, pp.817~9 (1985)
  13. "Role of the ATP-binding cassette (ABC), mitoxantrone-resistance half-transporter (MXR), and potential for glucuronidation in MXR-expressing cells", 59(23), pp.5938~5946 (1999)
  14. "Distribution of multi-drug resistance- associated P-glycoprotein in normal and neoplastic human tissues Analysis with 3 monoclonal antibodies recognizing different epitopes of the P-glycoprotein molecule", pp.56~64, Ketelaars H
  15. "The mouse Bcrp1", Mxr, pp.1999, 59(17)[DOI]
  16. , 2000, 3514~3521
  17. Scheffer GL, "The drug resistance-related protein LRP is the human major vault protein", 1, pp.578~582 (1995)
  18. Izquierdo MA, "Broad distribution of the multidrug resistance- related vault lung resistance protein in normal human tissues and tumors", 148, pp.877~887 (1996)
  19. Beck J, "Expression of mdr1, mrp, topoisomerase II alpha", beta, and cyclin A in primary or relapsed states of acute lymphoblastic leukaemias, 1995, pp.89(2), 356~363
  20. Newman EM, "Mechanisms of cross-resistance to methotrexate and 5-fluorouracil in an A2780 human ovarian carcinoma cell subline resistant to cisplatin", pp.443~7
  21. Takeishi K, "Nucleotide sequence of a functional cDNA for human thymidylate synthase", 13, pp.2035~43 (1985)
  22. Lambert E, "Cloning and sequencing a novel metallothionein I isoform expressed in human reticulocytes", 389(2), pp.210~212 (1996)
  23. Chernov I, "Characterization of the Wilson disease gene encoding a P-type copper transporting ATPase", ge(mic organization), pp.1647~56
  24. "Role of copper transporters in the uptake and efflux of platinum containing drugs", 234(1), pp.34~39 (2006)
  25. "Adaptation of the rapid automated tetrazolium dye based assay for chemosensitivity testing in childhood leukemia", pp.323~32
  26. "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction", pp.156~9
  27. "Molecular structure of the human cytoplasmic beta-actin gene interspecies homology of sequences in the introns", pp.6133~7
  28. "Remarks and conclusions on ovarian cancer treatment", 11, pp.77~81 (2001)
  29. "Reversal of P-glycoprotein-mediated multidrug resistance by protopanaxatriol ginsenosides from Korean red ginseng", 69(3), pp.235~240 (2003)
  30. "Purification of P-glycoprotein from plasma membrane vesicles of Chinese hamster ovary cell mutants with reduced colchicines permeability", pp.12701~5
  31. "P-glycoprotein expression is a marker for chemotherapy resistance and prognosis in advanced ovarian cancer", 20, pp.1061~1067 (2000)
  32. "Evaluation of chemoresistance markers in women with epithelial ovarian carcinoma", 81, pp.18~24 (2001)
  33. "Expression of multidrug resistance-associated markers, their relation to quantitative pathologic tumour characteristics and prognosis in advanced ovarian cancer", 24(1), pp.17~23 (2002)
  34. "A 190-kDa protein overexpressed in non-P- glycoprotein containing multidrug-resistant cells and its relationship to the MRP gene", 86, pp.110~7 (1994)
  35. "난소암 세포주와 cisplatin 내성주에서의 MDR 관련 유전자의 발현에 관한 연구", 44(9), pp.1633~1638 (2001)
  36. "Copper-transporting P-Type adenosine triphospatase (ATP7B) as a cisplatin based chemoresistance marker in ovarian carcinoma: Comparative analysis with expression of MDR1, MRP1, MRP2, LRP AND BCRP", 101, pp.488~495 (2002)
  37. "Analysis of the expression of MRP the gene for a new putative transmembrane drug transporter", pp.1747~50
  38. "Long-term cultivation of colorectal carcinoma cells with anti-cancer drugs induces drug resistance and telomere elongation an in vitro study Published", shi(miya n)

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