小靑龍湯이 생쥐의 肺 大食細胞 Cytokine 遺傳子 발현에 미치는 영향

Abstract

In many recent studies, molecular biological methods have been used to investigate the role of cytokines in pathogenesis of lung disease. This Experiment was conducted to investigate the effects of Sochungyong-tang on gene expressions in Mouse Alveolar Macrophage. For this purpose, we observed the cytokines (IL-1β, IL-6, IL-10, iNOS, MIP-1α, MIP-1β, MIP-1γ, TGF-β, TNF-α). We picked the alveolar macrophage out of mice and cultured it. We analyzed the cytokine gene expression by reverse transcription-PCR. The results obtained were as follows : 1. Sochungyong-tang showed inhibitory effects on IL-1β in time and concentration. 2. Sochungyong-tang showed inhibitory effects on IL-6 in time and concentration. 3. Sochungyong-tang showed inhibitory effects on IL-10 in concentration. 4. Sochungyong-tang showed inhibitory effects on iNOS. 5. Sochungyong-tang showed inhibitory effects on TGF-β in time and concentration. 6. Sochungyong-tang showed no inhibitory effects on MIP-1α, MIP-1β, MIP-1γ, TGF-β, TNF-α. According to above results, it is supposed that Sochungyong-tang has the inhibitory effects on cytokine gene expression in mouse alveolar macrophage and can be usefully applied for curing inflammatory process of lung disease. Advanced studies are required to investigate the cure mechanism of Sochungyong-tang in the future.