자궁경부암에서 Annexin I 및 Thymosin β4의 발현에 관한 연구
The expression of annexin I and thymosin β4 in cervical cancer
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=목적: Annexin I은 암세포의 침윤 및 맥관형성을 자극하는 인자이고, thymosin β4는 암의 전이와 맥관형성에 관여하는 인자로서, 자궁경부암에서 annexin I 및 thymosin β4의 발현에 관하여 알아보고자 하였다.방법: 33예의 침윤성 자궁경부암 환자와 10예의 자궁경부상피내종양 환자, 10예의 정상자궁경부 조직에서 QC-PCR과 western blot analysis를 이용하여 annxin I, thymosin β4 mRNA와 protein의 발현을 측정하였고, SiHa 세포주에 thymosin β4 peptide를 처리한 후 처리 전후의 annexin I의 발현 변화를 관찰하였다.결과: 자궁경부암에서 thymosin β4와 annexin I mRNA의 발현은 정상 자궁경부, 자궁경부상피내종양에 비해 통계적으로 유의하게 높았으며, thymosin β4와 annexin I 단백질도 정상자궁경부보다 자궁경부암에서 높게 발현되었다. Thymosin β4와 annexin I mRNA의 발현은 다른 예후인자와 유의한 상관관계가 없었으나, thymosin β4와 annexin I 단백질의 경우 임상병기가 진행될수록 발현이 증가하는 양상을 보였다. Thymosin β4 peptide를 처리한 SiHa 세포에서는 처리하지 않은 세포보다 annexin I의 발현이 약간 증가하는 소견을 보였다.
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