자궁에서의 혈관내피 산화질소 합성효소의 발현
The expression of endothelial nitric oxide synthase in the uterus
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목적: 산화질소는 반감기가 짧고 아질산염으로 쉽게 산화되어 활성도 측정이 쉽지 않기 때문에, 간접적인 방법을 통하여 산화질소가 월경 주기에 따른 영향과 자궁근종에 의한 차이를 보이는 지를 확인하여, 자궁에 영향을 주는 인자로 관여하는 지를 살펴보고자 한다. 연구 방법: 양성 질환으로 전자궁적출술을 받은 폐경 전 여성을 대상으로 근종만 진단된 경우를 실험군으로, 근종이나 다른 병변이 없었던 경우를 대조군으로 하였다. 각각을 면역조직화학염색을 시행하고, 증식기와 분비기로 분류하여 각 시기에 해당하는 실험군과 대조군을 비교하였다. 결과: 자궁내막과 자궁근육에서 혈관내피 산화질소 합성효소 발현은 대조군에 비해 실험군의 혈관내피세포에서 많이 되었으나 통계학적으로 유의하지 않았다 (P=0.319, P=0.264). 월경 주기에 따른 자궁내막의 혈관내피 산화질소 합성효소 발현 비교에서 증식기에는 대조군에서, 분비기에는 실험군에서 강하게 발현하였으나, 통계학적으로 유의한 차이는 보이지 않았다 (P=0.549, P=0.240). 월경 주기에 따른 자궁근육의 혈관내피 산화질소 합성효소 발현 비교에서 증식기에는 대조군에서, 분비기에는 실험군에서 더 강하게 발현되었으나 통계학적으로 유의한 차이를 보이지는 않았다 (P=0.279, P=0.224). 결론: 혈관내피 산화질소 합성효소는 자궁에서 자궁내막이나 자궁근육의 혈관 내피세포에서 발현하고, 통계학적으로 유의한 차이를 보이지 않았으나 자궁근종 환자에서 대조군에 비해 좀 더 많이 발현하고, 분비기에 좀 더 많이 발현하는 양상을 보이고 있다.
Abstract
Objective: Nitric oxide has a short half-life and is easily oxidized by nitrite, we can indirectly find out its activity by measuring the nitric oxide synthase in cells. The purpose of this study is to confirm the differences in the expression modes of eNOS in uterine endometrium and myometrium between the patients of uterine leiomyoma and the control group. Methods: We defined the patient group as perimenopausal women who were diagnosed with uterine leiomyoma and underwent total hysterectomy, and the control group as the women who had no lesions in uterus. All of them were classified into proliferative phase and secretory phase by the Noyes standards and compared by immunohistochemical stain for eNOS. Results: There is no significant differences between the patients of uterine leiomyoma and the control group statistically (endometrium P=0.319, myometrium P=0.264). The expression of eNOS in the vascular endothelial cells of the endometrium did not show significant differences statistically by menstrual cycle (proliferative phage P=0.549, secretory phage P=0.240). The expression of eNOS in the myometrium also did not show significant differences by menstrual cycle or by group statistically (proliferative phage P=0.279, secretory phage P=0.224). Conclusion: In this study, the expression of endothelial nitric oxide synthase was higher in the patients of uterine leiomyoma than in the control group, and during the secretory phase in menstrual period, but there was no statistical significance. We suppose that we can get statistically significant results if we have many cases of subjects.
참고문헌 (39)
- Moncada S, "Nitric oxide: physiology, pathophysiology, and pharmacology", Pharmacol Rev, 43, pp.109~142 (1991)
- Palmer RM, "Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor", Nature, 327, pp.524~526 (1987)
- Marletta MA, "Nitric oxide: biosynthesis and biological significance", Trends Biochem Sci, 14, pp.488~492 (1989)
- Snyder SH, "Nitric oxide. No endothelial NO", Nature, 377, pp.196~197 (1995)
- Albrecht EW, "Protective role of endothelial nitric oxide synthase", J Pathol, 199, pp.8~17 (2003)
- Telfer JF, "Expression of endothelial and inducible nitric oxide synthase in non-pregnant and decidualized human endometrium", Mol Hum Reprod, 3, pp.69~75 (1997)
- Knowles RG, "Nitric oxide synthases in mammals", Biochem J, 298, pp.249~258 (1994)
- Nathan C, "Nitric oxide as a secretory product of mammalian cells", FASEB J, 6, pp.3051~3054 (1992)
- Buhimschi I, "Differential regulation of nitric oxide in the rat uterus and cervix during pregnancy and labour", Hum Reprod, 11, pp.1755~1766 (1996)
- Telfer JF, "Identification of nitric oxide synthase in human uterus", Hum Reprod, 10, pp.19~23 (1995)
- Tseng L, "Cyclic expression of endothelial nitric oxide synthase mRNA in the epithelial glands of human endometrium", J Soc Gynecol Investig, 3, pp.33~38 (1996)
- Khorram O, "Endometrial and myometrial expression of nitric oxide synthase isoforms in pre- and postmenopausal women", J Clin Endocrinol Metab, 84, pp.2226~2232 (1999)
- Tschugguel W, "Induction of inducible nitric oxide synthase expression in human secretory endometrium", Hum Reprod, 13, pp.436~444 (1998)
- Ota H, "Endothelial nitric oxide synthase in the endometrium during the menstrual cycle in patients with endometriosis and adenomyosis", Fertil Steril, 69, pp.303~308 (1998)
- Taguchi M, "Endothelial nitric oxide synthase is differently expressed in human endometrial vessels during the menstrual cycle", Mol Hum Reprod, 6, pp.185~190 (2000)
- Buhimschi I, "Involvement of a nitric oxide-cyclic guanosine monophosphate pathway in control of human uterine contractility during pregnancy", Am J Obstet Gynecol, 172, pp.1577~1584 (1995)
- Norman J, "Nitric oxide and the myometrium", Pharmacol Ther, 70, pp.91~100 (1996)
- Myatt L, "The action of nitric oxide on the perfused human fetal placental circulation", Am J Obstet Gynecol, 164, pp.687~692 (1991)
- Waldman SA, "Biochemical mechanisms underlying vascular smooth muscle relaxation: the guanylate cyclase-cyclic GMP system", J Cardiovasc Pharmacol, 12(5), pp.S115~S118 (1988)
- Tsai AL, "How does NO activate hemoproteins?", FEBS Lett, 32, pp.141~145 (1994)
- Giles WB, "Fetal umbilical artery flow velocity wave forms and placntal resistence: pathologic correlation", Br J Obstet Gynecol, 92, pp.31~38 (1985)
- Jones CJP, "An ultrastructural and ultrahistochemical study of the human placenta in maternal preeclampsia", Placenta, 1, pp.61~76 (1980)
- Myatt L, "Failure of nitric oxide from the maternal intervillous space to regulate villous vascular reactivity in the fetal circulation of the human placenta in vitro", J Soc Gynecol Invest, 2, pp.258~271 (1995)
- Lyall F, "Nitric oxide concentrations are increased in the feto-placental circulation in intrauterine growth restriction", Placenta, 17, pp.165~168 (1996)
- Gokdeniz R, "Endothelial nitric oxide synthase expression in leiomyoma and parental myometrium", Gynecol Obstet Invest, 49, pp.132~136 (2000)
- Gokdeniz R, "GnRH agonist decreases endothelial nitric oxide synthase (eNOS) expression in leiomyoma", Int J Gynaecol Obstet, 70, pp.347~352 (2000)
- Veille JC, "Effects of estrogen on nitric oxide biosynthesis and vasorelaxant activity in sheep uterine and renal arteries in vitro", Am J Obstet Gynecol, 174, pp.1043~1049 (1996)
- Rosenfeld CR, "Nitric oxide contributes to estrogen-induced vasodilation of the ovine uterine circulation", J Clin Invest, 98, pp.2158~2166 (1996)
- Sutherland RS, "Vesicourethral function in mice with genetic disruption of neuronal nitric oxide synthase", J Urol, 157, pp.1109~1116 (1997)
- Hoyle CH, "Innervation of vasculature and microvasculature of the human vagina by NOS and neuropeptide-containing nerves", J Anat, 188, pp.633~644 (1996)
- Rein MS, "Fibroid and myometrial steroid receptors in women treated with gonadotropin-releasing hormone agonist leuprolide acetate", Fertil Steril, 53, pp.1018~1023 (1990)
- Otubu JA, "Unconjugated steroids in leiomyomas and tumor-bearing myometrium", Am J Obstet Gynecol, 143, pp.130~133 (1982)
- Deligdish L, "Endometrial changes associated with myomata of the uterus", J Clin Pathol, 23, pp.676~680 (1970)
- Brandon DD, "Progesterone receptor messenger ribonucleic acid and protein are overexpressed in human uterine leiomyomas", Am J Obstet Gynecol, 169, pp.78~85 (1993)
- Tang XM, "Insulin-like growth factor Ⅰ (IGF-Ⅰ), IGF-Ⅰ receptors, and IGF binding proteins 1-4 in human uterine tissue: tissue localization and IGF-I action in endometrial stromal and myometrial smooth muscle cells in vitro", Biol Reprod, 50, pp.1113~1125 (1994)
- TGF beta 1-binding sites, "Endocrinology", 1994, 439~449
- Rauk PN, "Mitogenic effect of basic fibroblast growth factor and estradiol on cultured human myometrial and leiomyoma cells", Am J Obstet Gynecol, 173, pp.571~577 (1995)
- Dou Q, "Suppression of transforming growth factor-beta (TGF beta) and TGF beta receptor messenger ribonucleic acid and protein expression in leiomyomata in women receiving gonadotropin-releasing hormone agonist therapy", J Clin Endocrinol Metab, 81, pp.3222~3230 (1996)
- Favini R, "Nitric oxide synthesis in human nonpregnant myometrium and uterine myomas", Fertil Steril, 79(1), pp.749~753 (2003)