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자궁내막증 자궁내막의 증가된 증식 활성도

Increased Proliferating Activity of Endometrium in Endometriosis

장두영|이응수|김영아|김미란*|박동욱†|안성희*|황경주*
대한산부인과학회 (Korean Soceity of Obstetrics and Gynecology)|2004.07|Obstetrics & Gynecology Science|제47권 제7호|pp. 1334-1341

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COX-2EndometriosisKi-67Endometrial polyp자궁내막증자궁내막용종NF- B
주제분류산부인과학

초록

목적 : 자궁내막증군과 정상 대조군에서 자궁경을 통한 병리조직 검사와 전사 인자 (transcription factor)인 NF-κB와 염증 매개인자 합성효소인 COX-2, 세포 증식 표지인자인 Ki-67의 면역조직화학적 검사를 통하여 증식 활성도를 비교하고자 하였다. 연구 방법 : 복강경 검사상 자궁내막증이 진단된 환자 92명을 실험군으로 설정하고 자궁내막증이 없는 정상 환자 90명을 대조군으로 하여 진단적 자궁경을 시행하였다. NF-κB, COX-2, Ki-67의 발현은 자궁내막증의 정상부위의 (eutopic) 자궁내막 10예와 정상 자궁내막 10예에서 조직을 채취하여 각각의 항체를 이용하여 면역조직화학적 염색을 시행했다. 결과 : 자궁내막증이 진단된 환자 중 자궁내막 용종이 동반된 환자는 전체 92명 중 53명 (57.6%)이었고 자궁내막증이 없는 환자 중 자궁내막 용종이 동반된 환자는 전체 90명 중 15명 (16.7%)으로 자궁내막증이 있는 경우에 자궁내막 용종이 동반된 경우가 유의하게 높게 나타났다. NF-κB (p65, p50), IκB, COX-2, Ki-67의 면역조직화학적 염색은 정상 자궁내막보다 자궁내막증의 자궁내막의 선상피와 기질세포에서 통계학적으로 의의 있게 발현되었다. 결론 : 자궁내막증의 정상부위 내막의 자궁내경을 통한 내막 조직검사로 용종이 증가되고 NF-κB, COX-2, Ki-67의 과발현으로 증식 활성도 (proliferation activity)가 증가됨을 확인하였다.

Abstract

Objective : We investigated the expression of Ki-67, NF-κB, and COX-2 in endometrial tissue, and the incidence of polyps by hysteroscopic biopsy with or without endometriosis. Methods : The study group was 92 patients with endometriosis and the control group was 90 patients without endometriosis. The subjects were 20 samples. The 10 samples consisted of eutopic endometrium with endometriosis. The control subjects were 10 samples of normal endometrium. Expression of Ki-67, NF-κB, and COX-2 was immunohistochemically investigated by polyclonal antibody. Results : Endometrial polyps were found in 53 of 92 (57.6%) women with endometriosis but only in 15 of 90 (16.7%) women without endometriosis. High expression of Ki-67 was shown in eutopic endometrium with endometriosis. The expression of NF-κB and COX-2 was increased in eutopic endometrium with endometriosis, but normal endometrium showed lower expression. Conclusion : The increase of endometrial polyps and hyperexpression of NF-κB, COX-2, Ki-67 could explain that proliferation activity of eutopic endometrium with endometriosis is increased.

참고문헌 (38)

  1. "Prevalence and anatomical distribution of endometriosis in women with selected gynaecological conditions: results from a multicentric Italian study. Gruppo italiano per lo studio dell'endometriosis", pp.1158~1162 (1994)
  2. "The development of the implantation theory for the origin of peritoneal endometriosis", 40, pp.549~557 (1940)
  3. "Pathogenesis of endometriosis based on endometrial homeoplasia, direct extension, exfoliation and implantation, lymphatic and hematogenous metaplasia. Including five case reports of endometrial tissue in pelvic lymph nodes", 2, pp.399~410 (1949)
  4. "A reappraisal of the coelomic metaplasia theroy by reviewing endometriosis occurring in unusual sites and instances", 165, pp.214~218 (1991)
  5. "Peritoneal endometriosis due to menstrual dissemination of endometrial tissue into the peritoneal cavity", 14, pp.422~429 (1927)
  6. "Altered mutation and function of peritoneal macrophages: possible role in the pathogenesis of endometriosis", 156, pp.783~785 (1987)
  7. "The NF- B and I B proteins: new discoreries and insights", 14, pp.649~683 (1996)
  8. "NF- B: a key role in inflammatory diseases", 107, pp.7~11 (2001)
  9. "Tumor necrosis factor-alpha-induced interleukin-8(IL-8) expression in endometriotic stromal cells, probably through nuclear factor-kappa B activation:gonadotropin-releasing hormone agonist treatment reduced IL-8 expression", 88, pp.730~735 (2003)
  10. "Nuclear Factor- B Regulate Cyclooxygenase- 2 expression and cell proliferattion in Human Gastric Cancer cells", 81, pp.349~360 (2001)
  11. "Hypoxia induces cyclooxygenase-2 via the NF- B p65 transcription factor in human vascular endothelial cells", 272, pp.601~608 (1997)
  12. "Cyclooxygenases: structural, cellular, and molecular biology", 69, pp.145~182 (2000)
  13. "Up-regulation of cyclooxygenase 2 gene expression in human colorectal adenomas and adenocarcinomas", 107, pp.1183~1188 (1994)
  14. "Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis", 74, pp.760~766 (2000)
  15. "Oral contraceptives suppress cell proliferation and enhance apoptosis of eutopic endometrial tissue from patients with endometriosis", 77, pp.1141~1147 (2002)
  16. "Bcl-2 heterodimerises in vivo with a conserved homologue Bax that accelerates programmed cell death", 74, pp.609~619 (1993)
  17. "Aromatase: a key molecule in the pathophysiology of endometriosis and a therapeutic target", 72, pp.961~969 (1999)
  18. "Molecular characterization of the gene locus of the human cell proliferation- associated nuclear protein defined by monoclonal antibody Ki-67", 29, pp.1~12 (1996)
  19. "The Ki-67 protein; Fascinating forms and an unknown function", 257, pp.231~7237 (2000)
  20. "Revised American Fertility Society Classification of Endometriosis", 115, pp.79~83 (1985)
  21. "HLA-DR expression in endometriotic tissue in patients with endometriosis and adenomyosis", 60, pp.834~838 (1993)
  22. "Immnuno-localization and regulation of the chemokine RANTES in the human endometrial and endometriosis tissue and cells", 82, pp.1612~1628 (1997)
  23. "Vascular endothelial growth factor and endometriotic angiogenesis", 6, pp.45~55 (2000)
  24. "Localization in tissues and secretion of eotaxin by cells from normal endometrium and endometriosis", 85, pp.2604~2607 (2000)
  25. "Expression of intercellular adhesion molecule(ICAM)-1 mRNA and protein is enhanced in endometriosis versus endometrial stromal cells in culture", 5(p), pp.642~650 (1998)
  26. "Women with endometriosis show a defect in natural killer cell activity resulting in a decreased cytotoxicity to autologous endometrium", 56, pp.45~51 (1991)
  27. "Steroid and growth factor regulation of matrix metalloproteinase expression in endometriosis", 15, pp.301~308 (1996)
  28. "Mutiple nuclear factors interact with the immunoglobulin enhancer sequences", 46, pp.705~716 (1986)
  29. "Mapping of the inducible I(kappa )B phosphorylation sites that signal its ubiquitination and degradation", 16, pp.1295~1304 (1996)
  30. "Cyclooxygenase-2 overexpression and tumor formation are blocked by sulindac in a murine model of familial adenomatous polyposis", 56, pp.2556~2560 (1996)
  31. "Nuclear Factor- B activity is down-regulated in nasal polyps from aspirin- sensitive asthmatics", 58, pp.122~126 (2003)
  32. "Eicosanoid production by the human gastric cancer cell line AGC and its relation to cell growth", 152, pp.1744~1749 (1992)
  33. "Prostaglandin F2α synthesis and metabolism by luteal phase endometrium in vitro", 52, pp.769~773 (1989)
  34. "Effects of peritoneal macrophages from women with endometriosis on endometrial cellular proliferation in an in vitro coculture model", 72, pp.533~538 (1999)
  35. "Monoclonal antibody Ki 67: its use in histopathology", 17, pp.489~501 (1990)
  36. "Immunohistochemical analysis of proliferative activity and steroid receptor expression in peritoneal and ovarian endometriosis", 68, pp.912~919 (1997)
  37. "PGE2 and PGF2α release by human peritoneal macrophages in endometriosis", 51, pp.49~60 (1996)
  38. "Progesterone-growth factor interactions in uterine stromal cells", 59, pp.464~469 (1998)

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