乾漆이 혈관신생관련 단백질 발현에 미치는 영향

Abstract

Objective : Angiogenesis is an essential process for metastasis of solid tumors and Psoriasis. Lots of Researches for anti-angiogenic effect to angiogenic factors have been carried out in the world. So this experiment was carried out for whether Lacca Sinica Exsiccata(LSE) extracts have an anti-angiogenic effect for angiogenic factors. Methods : To investigate the roles of the LSE extracts, we performed MTS assay, western blots using HaCaT cells and HepG2 cells. And then, HaCaT cells were treated with 10, 50, 100, 250, 500㎍/㎖ LSE extracts. After 4hrs, HaCaT cells were theated with IGF-II protein for 1hr. HepG2 cells were treated with 1, 10, 25, 50, 100, 200 ㎍/㎖ LSE extracts. After 4hrs, HepG2 cells were theated with CoCl2 for 24hrs. Results : 1. In 50㎍/㎖ and 100㎍/㎖ density we confirmed the inhibition effect of LSE extracts to HIF-1α activation which was induced by IGF-II in HaCaT cells. 2. In 25㎍/㎖ density we confirmed the inhibition effect of LSE extracts to HIF-1α activation which was induced by CoCl2 in HepG2 cells. 3. In 25㎍/㎖ density we confirmed the inhibition effect of LSE extracts to VEGF activation which was induced by CoCl2 in HepG2 cells. Conclusion : The above-mentioned results proved that LSE extracts reduced HIF-1α protein level in the HaCaT cells and HepG2 cells. These results suggest that inhibition of HaCaT cell and HepG2 cell proliferation by LSE extracts contributes to the anti-angiogenic activities on the keratinocytes and hepatocellular carcinoma.