生地黃이 혈관신생, 세포생존 및 염증관련 단백질발현에 미치는 영향

Abstract

Objective : Angiogenesis induced by hypoxia and inflammation are an essential process for the metastasis of solid tumors and psoriasis. We researched the HIF-1α(hypoxia inducible factor 1 alpha), VEGF(Vascular Endothelial Growth Factor), survival related PI3K-Akt, and inflammation related COX-2 protein expressions to get the information of the mechanism and effects of Rehmannia glutinosa in HepG2 and HaCaT cell lines. Method : To investigate the roles of the Rehmannia glutinosa extracts, we performed MTS assay and western blots using HaCaT cells and HepG2 cells. HaCaT cells and HepG2 cells were treated with 50㎍/㎖ and 100㎍/㎖ Rehmannia glutinosa extracts. After 4hrs, HaCaT cells were treated with IGF-II protein for 24hrs and HepG2 cells were treated with CoCl2. Results : 1. We could observe that the reduction of the protein level of HIF-1α induced by IGF-Ⅱ in HaCaT cells. 2. We could observe that the decreased PI3K-Akt and COX-2 expression level by Rehmannia glutinosa extracts treated in HaCaT cells independently with ERK1/2. 3. We could observe that the reduction of the protein level of HIF-1α induced by CoCl2 in HepG2 cells. Conclusion : These results suggest that Rehmannia glutinosa extracts contributes to the anti-survival pathway and anti-inflammatory activities. Also, we could assume that Rehmannia glutinosa act as anti-inflammatory or anti-hypoxia agents via reduction of COX-2 and HIF-1α.