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Dose-dependent embryotrophic effect of recombinant granulocyte-macrophage colony-stimulating factor and brain-derived neurotrophic factor in culture medium for mouse preimplantation embryo

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ObjectiveTo evaluate the dose effect of recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) orbrain-derived neurotrophic factor (BDNF) in culture medium on the development of in vitro fertilized mouse embryos. MethodsMature oocytes were retrieved from superovulated female BDF1 mice and inseminated by sperm from male BDF1mice. On day 1, two-cell stage embryos were divided and cultured until day 5 in the embryo maintenance mediumsupplemented with 0, 1, 2, 5, or 10 ng/mL of rmGM-CSF or supplemented with 0, 5, 10, or 20 ng/mL of BDNF. Blastocystformation rate and their cell numbers were assessed. ResultsThe blastocyst formation rate and the total cell count in blastocyst was similar in all the rmGM-CSF treatment groupswhen compared with the control. However, the blastocyst formation rate and the total cell count was significantlyhigher in the group supplemented with 10 ng/mL of BDNF compared with the control (63.9%, 45.8±11.5 vs. 52.3%,38.0±6.8; P<0.05, respectively). ConclusionSupplementation of 10 ng/mL of BDNF enhanced the developmental potential of mouse preimplantation embryos,but supplementation of rmGM-CSF did not.

Abstract

ObjectiveTo evaluate the dose effect of recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) orbrain-derived neurotrophic factor (BDNF) in culture medium on the development of in vitro fertilized mouse embryos. MethodsMature oocytes were retrieved from superovulated female BDF1 mice and inseminated by sperm from male BDF1mice. On day 1, two-cell stage embryos were divided and cultured until day 5 in the embryo maintenance mediumsupplemented with 0, 1, 2, 5, or 10 ng/mL of rmGM-CSF or supplemented with 0, 5, 10, or 20 ng/mL of BDNF. Blastocystformation rate and their cell numbers were assessed. ResultsThe blastocyst formation rate and the total cell count in blastocyst was similar in all the rmGM-CSF treatment groupswhen compared with the control. However, the blastocyst formation rate and the total cell count was significantlyhigher in the group supplemented with 10 ng/mL of BDNF compared with the control (63.9%, 45.8±11.5 vs. 52.3%,38.0±6.8; P<0.05, respectively). ConclusionSupplementation of 10 ng/mL of BDNF enhanced the developmental potential of mouse preimplantation embryos,but supplementation of rmGM-CSF did not.

참고문헌 (31)

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