The Effect of Fibroblast Co-culture on In Vitro Maturation of Mouse Preantral Follicles
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This study was performed to evaluate the effects of fibroblast co-culture on in vitro maturation (IVM) of prepubertal mouse preantral follicles. The intact preantral follicles were obtained from the ovaries of 12－14 day old mice and these were cultured individually in α-minimal essential medium (α-MEM) supplemented with 5% fetal bovine serum (FBS), 100 mIU/㎖ recombinant follicle stimulating hormone (rFSH), 1% insulin-transferrin-selenium, 100 μg/ml penicillin and 50 ㎍/㎖ streptomycin as base medium for 12 days. A total of 200 follicles were cultured in base medium co-cultured with mouse embryonic fibroblast (MEF) (MEF group) (n=100) or only base medium as control group (n=100). Survival rate of follicles on day 12 of culture were significantly higher in the MEF group of 90.0%, compared with 77.0% of the control group (p=0.021). Follicle diameters on day 6 and 8 of the culture period were significantly larger in the MEF group than those in the control group (p=0.021, p=0.007, respectively). Estradiol levels in culture media on day 4, 6, 8, 10 and 12 of the culture period were significantly higher in the MEF group (p=0.043, p=0.021, p=0.006, p<0.001 and p=0.008, retrospectively). Our data suggest that MEF cell co-culture on IVM of mouse preantral follicle increases survival rate and promotes follicular growth and steroid production.
Abstract
This study was performed to evaluate the effects of fibroblast co-culture on in vitro maturation (IVM) of prepubertal mouse preantral follicles. The intact preantral follicles were obtained from the ovaries of 12－14 day old mice and these were cultured individually in α-minimal essential medium (α-MEM) supplemented with 5% fetal bovine serum (FBS), 100 mIU/㎖ recombinant follicle stimulating hormone (rFSH), 1% insulin-transferrin-selenium, 100 μg/ml penicillin and 50 ㎍/㎖ streptomycin as base medium for 12 days. A total of 200 follicles were cultured in base medium co-cultured with mouse embryonic fibroblast (MEF) (MEF group) (n=100) or only base medium as control group (n=100). Survival rate of follicles on day 12 of culture were significantly higher in the MEF group of 90.0%, compared with 77.0% of the control group (p=0.021). Follicle diameters on day 6 and 8 of the culture period were significantly larger in the MEF group than those in the control group (p=0.021, p=0.007, respectively). Estradiol levels in culture media on day 4, 6, 8, 10 and 12 of the culture period were significantly higher in the MEF group (p=0.043, p=0.021, p=0.006, p<0.001 and p=0.008, retrospectively). Our data suggest that MEF cell co-culture on IVM of mouse preantral follicle increases survival rate and promotes follicular growth and steroid production.
참고문헌 (19)
- Berisha B, "Expression, tissue concentration and localisation of some angiogenic factors during the final growth of bovine ovarian follicles", Exp Clin Endocrinol Diabetes, 109(Suppl 1), pp.67~ (2001)
- Bishonga C, "In vitro growth of ovarian peantral follicles and the capacity of their oocytes to develop to the blastocyst stage", J Vet Med Sci, 63, pp.619~624 (2001)
- Cortvrindt R, "In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepubertal mice in a simplified culture system", Hum Reprod, 11(12), pp.2656~2666 (1996)
- Cortvrindt R, "Recombinant luteinizing hormone as a survival and differentiation factor increases oocyte maturation in recombinant follicle stimulating hormone-supplemented mouse preantral follicle culture", Hum Reprod, 13(5), pp.1292~1302 (1998)
- Demeestere I, "Impact of various endocrine and paracrine factors on in vitro culture of preantral follicles in rodents", Reproduction, 130, pp.147~156 (2005)
- Eppig JJ, "Intercommunication between mammalian oocytes and companion somatic cells", Bioassays, 13(11), pp.569~574 (1991)
- Haidari K, "The effect of leukemia inhibitory factor and coculture on the in vitro maturation and ultrastructure of vitrified and nonvitrified isolated mouse preantral follicles", Fertil Steril, 90(6), pp.2389~2397 (2008)
- Hajializadeh N, "The development of mouse early embryos in vitro in fibroblasts and cumulus cells co-cultures supplemented with retinoic acid", Iranian J Vet Res, 9(1), pp.1~8 (2008)
- Hatoya S, "Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocyte", Theriogenology, 66(5), pp.1083~1090 (2006)
- Malekshah AK, "Follicular fluid and cumulus cells synergistically improve mouse early embryo development in vitro", J Reprod Dev, 51(2), pp.195~199 (2005)
- Manova K, "The expression pattern of the c-kit ligand in gonads of mice supports a role for the c-kit receptor in oocyte growth and in proliferation of spermatogonia", Dev Biol, 157, pp.85~99 (1993)
- Mao J, "Effects of culture medium, serum type and various concentrations of follicule stimulating hormone on porcine preantral follicular development and antrum formation in vitro", Biol Reprod, 67(4), pp.1197~1203 (2002)
- McGee EA, "Keratinocyte growth factor promotes the survival, growth and differentiation of preantral follicles", Fertil Steril, 71(4), pp.732~738 (1999)
- Nilsson E, "Basic fibroblast growth factor induces primordial follicle development and initiates folliculogenesis", Mol Cell Endocrinol, 175, pp.123~130 (2001)
- Rubin JS, "Purification and characterization of a newly identified growth factor specific for epithelial cells", Proc Natl Acad Sci USA, 86, pp.802~806 (1989)
- Seghezzi G, "Fibroblast growth factor-2 (FGF-2) induces vascular endothelial growth factor (VEGF) expression in the endothelial cells of forming capillaries: An autocrine mechanism contributing to angiogenesis", J Cell Biol, 141, pp.1659~1673 (1998)
- Shikone T, "Follicle stimulating hormone induces functional receptors for basic fibroblast growth factor in rat granulosa cells", Endocrinology, 131, pp.1063~1068 (1992)
- Sun F, "Preantral follicle culture as a novel in vitro assay in reproductive toxicology testing in mammalian oocytes", Mutagenesis, 19, pp.13~25 (2004)
- Tan XW, "Effects of species and cellular activity of oviductal epithelial cells on their dialogue with cocultured mouse embryos", Cell Tissue Res, 327, pp.55~66 (2007)