수종의 청열약물의 미백 및 항알러지 효과에 대한 실험적 연구
Experimental studies on the Whitening and Anti-allergic effect of various Herbal medicines that clear heat
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Abstract
Objective : In this study, herbal medicine(GJE, Gardenia jasminoides Ellis; HCT, Houttuynia cordata Thunb.; CIL, Chrysanthemum indicum Linne; PMS,Paeonia moutan Sims, P. subfruticosa Makino; APL, Agrimonia pilosa Ledebour) were screened for their inhibitory activities against Tyrosinase and PMA plus A23187-induced TNF-α, IL-6, IL-8 productions in HMC-1 cells to reveal their skin -whitening and anti-allergic effect. Method : To investigate Tyrosinase inhibition we treated Mushroom Tyrosinase(Fluka, 93898) 10㎕ and 7.5mM Tyrosine (Sigma, T3754)20㎕ with 80% ethanol medicine extracts. Then we observed 96well micro plate extinction at 490nm. In the next experiment, to investigate Anti-allergic effect we blended cultured Human Mast Cells(HMC-1) with medicine extracts. We treated the blended solution with Phorbol 12-myristate 13-acetate(PMA) and A23187, then observed TNF-α, IL-6, IL-8 by ELISA (enzyme-linked immunosorbent assay) at 450nm. Results : In inhibiting Tyrosinase the results are as follows. 1.We observed 22% inhibition of Mushroom Tyrosinase at 500 ㎍/㎖ concentration of GJE extracts. 2.We also could observe that the decreased Mushroom Tyrosinase activities in HCT, CIL extracts. In inhibiing TNF-α, IL-6, IL-8 productions in HMC-1 cells the results are as follows. 1.Of the extracts examined, HCT, PMS, APL extracts showed over 50% inhibitions of Cytokines at 200 ㎍/㎖ concentration. 2.In particular, APL extracts showed the best inhibitory effect on Cytokine productions in a dose-dependent manner. Conclusion:These results suggest that GJE extracts contributes to the anti-melanin activities and represent a potential source of whitening agent. Thus these herbal medicines suggest novel drugs on anti-allergic effects.
참고문헌 (18)
- "Screening of national products that have activities Skin-anging", 14(6), pp.568~572 (2001)
- "Inhibitory effects of plant extracts on Tyrosinase, L-DOPA oxidation and melanoma systhesis", 70, pp.393~407 (2007)
- "Bio chemical control of melanogenesis and melanomal organization", 4, pp.24~ (1999)
- "T Fuction and skin depigmental activity of crude drugs", pp.56~
- "A study on Antioxidative Effect of Leonury Herba extract", 31(2), pp.189~196 (2005)
- "Pharmacological properties of traditional medicine. ？뵤？, Pharmacokinetic study of mulberroside A and its metabolites in rat", 19(11), pp.1463~ (1996)
- "Mast cells can amplify airway reactivity and features of chronic inflammation in an asthma model in mice", 192, pp.455~462 (2000)
- "PW Initiation of delayed-type hypersensitivity by low doses of monoclonal IgE antibody. Mediation by serotonin and inhibition by histamine. J. Immunol. 1991", pp.3929~36
- "Tumour necrosis factor-alpha: the role of this multifunctional Cytokine in asthma", 79, pp.132~140 (2001)
- "Mast cells enhance T cell activation: importance of mast cell-derived TNF", 102, pp.6467~6472 (2005)
- "Phosphatase inhibition potentiates IL-6 production by mast cells in response to Fcepsilon RI-mediated activation: involvement of p38 MAPK", 76, pp.1075~1081 (2004)
- "The immediate early gene 1 product of human cytomegalovirus is sufficient for up-regulation of interleukin-8 gene expression", 279(1), pp.298~304 (2000)
- "The study on the whitening effect of Ephedra sinica Extract", 31(2), pp.153~159 (2005)
- "？穉？？？W세？g놔경？？. 1977 translated by Kim", pp.2525~
- Lee, SJ, "Screening of plants in jeju for whitening materials in cosmeceutical", 31(1), pp.115~119 (2005)
- Bernard, P, "Resveratol: an original mechanism on Tyrosinase inhibition", 22, pp.219~226 (2000)
- RPA, "PGH Clinival Aspects of Immunology", pp.317~37 (1963)
- Janeway, CA, "in Immunobiology: The Immune System in Health and Disease", pp.461~488 (1999), Current Biology Publication, London