Characterizing Helicobacter pylori cagA in Myanmar
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Background/Aims: Differences in the Helicobacter pylori infection rate are not sufficient to clarify the dissimilarity of gastric cancer incidence between Myanmar and its neighboring countries. To better understand this trend, the H. pylori virulence gene cagA was characterized in Myanmar. Methods: Glutamate-proline-isoleucine-tyrosine-alanine (EPIYA) patterns and CagA multimerization (CM) motifs of cagA genotypes were examined by performing polymerase chain reactions and DNA sequencing. Results: Of 69 tested H. pylori strains, cagA-positive patients had significantly more severe histological scores in their antrum than cagA-negative patients. Sequence analysis revealed that 94.1% of strains had Western-type cagA containing an EPIYA motif (92.6%) or EPIYT motif (6.4%). The intestinal metaplasia scores in the antral of patients infected with the ABC and ABCC types of cagA were significantly higher than those of patients with AB-type cagA. Interestingly, in patients infected with H. pylori, 46.3% of strains with three EPIYA motifs contained two identical Western-typical CM motifs, and these patients showed significantly higher antrum inflammation scores than patients infected with two identical nontypical-CM motif strains (p=0.02). Conclusions: In Myanmarese strains, Western-type cagA was predominant. The presence of CM motifs and the proportion of multiple EPIYA-C segments might partially explain the intermediate gastric cancer risk found in Myanmar.
Abstract
Background/Aims: Differences in the Helicobacter pylori infection rate are not sufficient to clarify the dissimilarity of gastric cancer incidence between Myanmar and its neighboring countries. To better understand this trend, the H. pylori virulence gene cagA was characterized in Myanmar. Methods: Glutamate-proline-isoleucine-tyrosine-alanine (EPIYA) patterns and CagA multimerization (CM) motifs of cagA genotypes were examined by performing polymerase chain reactions and DNA sequencing. Results: Of 69 tested H. pylori strains, cagA-positive patients had significantly more severe histological scores in their antrum than cagA-negative patients. Sequence analysis revealed that 94.1% of strains had Western-type cagA containing an EPIYA motif (92.6%) or EPIYT motif (6.4%). The intestinal metaplasia scores in the antral of patients infected with the ABC and ABCC types of cagA were significantly higher than those of patients with AB-type cagA. Interestingly, in patients infected with H. pylori, 46.3% of strains with three EPIYA motifs contained two identical Western-typical CM motifs, and these patients showed significantly higher antrum inflammation scores than patients infected with two identical nontypical-CM motif strains (p=0.02). Conclusions: In Myanmarese strains, Western-type cagA was predominant. The presence of CM motifs and the proportion of multiple EPIYA-C segments might partially explain the intermediate gastric cancer risk found in Myanmar.
참고문헌 (37)
- Sahara S, "Role of Helicobacter pylori cagA EPIYA motif and vacA genotypes for the development of gastrointestinal diseases in Southeast Asian countries: a meta-analysis", BMC Infect Dis, 12, pp.223~ (2012)[DOI]
- Sodhi JS, "Prevalence of Helicobacter pylori infection and the effect of its eradication on symptoms of functional dyspepsia in Kashmir, India", J Gastroenterol Hepatol, 28, pp.808~813 (2013)[DOI]
- Myint T, "Prevalence of Helicobacter pylori infection and atrophic gastritis in patients with dyspeptic symptoms in Myanmar", World J Gastroenterol, 21, pp.629~636 (2015)[DOI]
- Yamaoka Y, "Mechanisms of disease: Helicobacter pylori virulence factors", Nat Rev Gastroenterol Hepatol, 7, pp.629~641 (2010)[DOI]
- Matsunari O, "Association between Helicobacter pylori virulence factors and gastroduodenal diseases in Okinawa, Japan", J Clin Microbiol, 50, pp.876~883 (2012)[DOI]
- Backert S, "Phosphorylation of tyrosine 972 of the Helicobacter pylori CagA protein is essential for induction of a scattering phenotype in gastric epithelial cells", Mol Microbiol, 42, pp.631~644 (2001)
- Higashi H, "Biological activity of the Helicobacter pylori virulence factor CagA is determined by variation in the tyrosine phosphorylation sites", Proc Natl Acad Sci U S A, 99, pp.14428~14433 (2002)[DOI]
- Selbach M, "Src is the kinase of the Helicobacter pylori CagA protein in vitro and in vivo", J Biol Chem, 277, pp.6775~6778 (2002)[DOI]
- Higashi H, "SHP-2 tyrosine phosphatase as an intracellular target of Helicobacter pylori CagA protein", Science, 295, pp.683~686 (2002)[DOI]
- Yamaoka Y, "Variants of the 3' region of the cagA gene in Helicobacter pylori isolates from patients with different H. pylori-associated diseases", J Clin Microbiol, 36, pp.2258~2263 (1998)
- Yamaoka Y, "Relationship between the cagA 3' repeat region of Helicobacter pylori, gastric histology, and susceptibility to low pH", Gastroenterology, 117, pp.342~349 (1999)[DOI]
- Xia Y, "A comprehensive sequence and disease correlation analyses for the C-terminal region of CagA protein of Helicobacter pylori", PLoS One, 4, pp.e7736~ (2009)[DOI]
- Zhang XS, "A specific A", T polymorphism in Western tyrosine phosphorylation B-motifs regulates Helicobacter pylori CagA epithelial cell interactions, 2015, pp.11, e1004621~
- Miftahussurur M, "Helicobacter pylori virulence genes in the five largest islands of Indonesia", Gut Pathog, 7, pp.26~ (2015)[DOI]
- El-Etr SH, "Phosphorylation-independent effects of CagA during interaction between Helicobacter pylori and T84 polarized monolayers", J Infect Dis, 190, pp.1516~1523 (2004)[DOI]
- Ren S, "Structural basis and functional consequence of Helicobacter pylori CagA multimerization in cells", J Biol Chem, 281, pp.32344~32352 (2006)[DOI]
- Sicinschi LA, "CagA C-terminal variations in Helicobacter pylori strains from Colombian patients with gastric precancerous lesions", Clin Microbiol Infect, 16, pp.369~378 (2010)[DOI]
- Suzuki M, "Helicobacter pylori CagA phosphorylation-independent function in epithelial proliferation and inflammation", Cell Host Microbe, 5, pp.23~34 (2009)[DOI]
- Dixon MF, "Classification and grading of gastritis: the updated Sydney system. International Workshop on the Histopathology of Gastritis, Houston 1994", Am J Surg Pathol, 20, pp.1161~1181 (1996)
- Yamaoka Y, "Relationship of vacA genotypes of Helicobacter pylori to cagA status, cytotoxin production, and clinical outcome", Helicobacter, 3, pp.241~253 (1998)[DOI]
- Suzuki R, "Molecular epidemiology, population genetics, and pathogenic role of Helicobacter pylori", Infect Genet Evol, 12, pp.203~213 (2012)[DOI]
- Yamaoka Y, "Relationship between Helicobacter pylori iceA, cagA, and vacA status and clinical outcome: studies in four different countries", J Clin Microbiol, 37, pp.2274~2279 (1999)
- van Doorn LJ, "Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori", Gastroenterology, 115, pp.58~66 (1998)[DOI]
- Mukhopadhyay AK, "Distinctiveness of genotypes of Helicobacter pylori in Calcutta, India", J Bacteriol, 182, pp.3219~3227 (2000)[DOI]
- Yamaoka Y, "Helicobacter pylori in North and South America before Columbus", FEBS Lett, 517, pp.180~184 (2002)[DOI]
- Summerer M, "Large-scale mitochondrial DNA analysis in Southeast Asia reveals evolutionary effects of cultural isolation in the multi-ethnic population of Myanmar", BMC Evol Biol, 14, pp.17~ (2014)[DOI]
- Vilaichone RK, "Molecular epidemiology and outcome of Helicobacter pylori infection in Thailand: a cultural cross roads", Helicobacter, 9, pp.453~459 (2004)[DOI]
- Chomvarin C, "Prevalence of cagA EPIYA motifs in Helicobacter pylori among dyspeptic patients in northeast Thailand", Southeast Asian J Trop Med Public Health, 43, pp.105~115 (2012)
- Tiwari SK, "Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome", Genet Mol Biol, 34, pp.280~285 (2011)[DOI]
- Argent RH, "Determinants and consequences of different levels of CagA phosphorylation for clinical isolates of Helicobacter pylori", Gastroenterology, 127, pp.514~523 (2004)[DOI]
- Azuma T, "Correlation between variation of the 3' region of the cagA gene in Helicobacter pylori and disease outcome in Japan", J Infect Dis, 186, pp.1621~1630 (2002)[DOI]
- Basso D, "Clinical relevance of Helicobacter pylori cagA and vacA gene polymorphisms", Gastroenterology, 135, pp.91~99 (2008)[DOI]
- Batista SA, "Higher number of Helicobacter pylori CagA EPIYA C phosphorylation sites increases the risk of gastric cancer, but not duodenal ulcer", BMC Microbiol, 11, pp.61~ (2011)[DOI]
- Mitacek EJ, "Geographic distribution of liver and stomach cancers in Thailand in relation to estimated dietary intake of nitrate, nitrite, and nitrosodimethylamine", Nutr Cancer, 60, pp.196~203 (2008)[DOI]
- Lim KG, "Malays in peninsular Malaysia may have the lowest incidence of stomach cancer in the world", Med J Malaysia, 64, pp.91~92 (2009)
- Tassaneeyakul W, "CYP2C19 genetic polymorphism in Thai, Burmese and Karen populations", Drug Metab Pharmacokinet, 21, pp.286~290 (2006)[DOI]
- Lunet N, "Helicobacter pylori infection and gastric cancer: facing the enigmas", Int J Cancer, 106, pp.953~960 (2003)[DOI]
