Genetic Instability in Gastric Epithelial Neoplasias Categorized by the Revised Vienna Classification
2
조회
0
다운로드
2
인용
0
참고문헌
초록
Background/Aims: The aim of this study was to determine the structural chromosomal aberrations, such as loss of heterozygosity (LOH) and microsatellite instability (MSI), at multiple tumor suppressor gene loci in gastric epithelial neoplasia categorized by the revised Vienna classification. Methods: All tissue samples were excised by endoscopic mucosal resection. Sixty category 3 (low-grade adenoma) tissue samples and 51 category 4 samples (high-grade adenoma and intramucosal carcinoma with adenoma) were examined at the 7 sets of microsatellite loci linked to the tumor suppressor gene locus. Results: For category 3 and 4 tissue samples, there were no differences in the frequencies of LOH-positive chromosomes or the extent of chromosomal loss. The Helicobacter-pylori (H. pylori)-positive rate was significantly higher in MSI-positive category 4 samples than in category 3 samples (p=0.04). The frequency of MSI positivity was significantly higher in category 4 samples than in category 3 samples (p=0.003). Conclusions: H. pylori infection is associated with genetic instability of the premalignant lesion. MSI occurs in the early stages of gastric carcinogenesis and its occurrence increases during malignant transformation. Detection of MSI in premalignant gastric lesions may be a surveillant of risk of malignant transformation.
Abstract
Background/Aims: The aim of this study was to determine the structural chromosomal aberrations, such as loss of heterozygosity (LOH) and microsatellite instability (MSI), at multiple tumor suppressor gene loci in gastric epithelial neoplasia categorized by the revised Vienna classification. Methods: All tissue samples were excised by endoscopic mucosal resection. Sixty category 3 (low-grade adenoma) tissue samples and 51 category 4 samples (high-grade adenoma and intramucosal carcinoma with adenoma) were examined at the 7 sets of microsatellite loci linked to the tumor suppressor gene locus. Results: For category 3 and 4 tissue samples, there were no differences in the frequencies of LOH-positive chromosomes or the extent of chromosomal loss. The Helicobacter-pylori (H. pylori)-positive rate was significantly higher in MSI-positive category 4 samples than in category 3 samples (p=0.04). The frequency of MSI positivity was significantly higher in category 4 samples than in category 3 samples (p=0.003). Conclusions: H. pylori infection is associated with genetic instability of the premalignant lesion. MSI occurs in the early stages of gastric carcinogenesis and its occurrence increases during malignant transformation. Detection of MSI in premalignant gastric lesions may be a surveillant of risk of malignant transformation.
참고문헌 (53)
- Fuchs CS, "Gastric carcinoma", N Engl J Med, 333, pp.32~41 (1995)
- Correa P, "Human gastric carcinogenesis: a multistep and multifactorial process--First American cancer society award lecture on cancer epidemiology and prevention", Cancer Res, 52, pp.6735~6740 (1992)
- Hiyama T, "In situ mRNA hybridization technique for analysis of human telomerase RNA in gastric precancerous and cancerous lesions", Jpn J Cancer Res, 89, pp.1187~1194 (1998)
- Tahara E, "Molecular mechanism of stomach carcinogenesis", J Cancer Res Clin Oncol, 119, pp.265~272 (1993)
- Loeb LA, "Microsatellite instability: marker of a mutator phenotype in cancer", Cancer Res, 54, pp.5059~5063 (1994)
- Lengauer C, "Genetic instability in colorectal cancers", Nature, 386, pp.623~627 (1997)
- Thibodeau SN, "Microsatellite instabilityin cancer of the proximal colon", Science, 260, pp.816~819 (1993)
- Ionov Y, "Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis", Nature, 363, pp.558~561 (1993)
- Kamiya T, "Long-term follow-up study on gastric adenoma and its relation to gastric protruded carcinoma", Cancer, 50, pp.2496~2503 (1982)
- Orlowska J, "Malignant transformation of benign epithelial gastric polyps", Am J Gastroenterol, 90, pp.2152~2159 (1995)
- Lauwers GY, "Gastric epithelial dysplasia", Gut, 45, pp.784~790 (1999)
- Schlemper RJ, "Differences in diagnostic criteria for gastric carcinoma between Japanese and western pathologists", Lancet, 349, pp.1725~1729 (1997)
- Schlemper RJ, "The Vienna classification of gastrointestinal epithelial neoplasia", Gut, 47, pp.251~255 (2000)
- Stolte M, "The new Vienna classification of epithelial neoplasia of the gastrointestinal tract: advantages anddisadvantages", Virchows Arch, 442, pp.99~106 (2003)
- Kim KM, "Genetic classification of intestinal-type and diffuse-type gastric cancers based on chromosomal loss and microsatellite instability", Virchows Arch, 443, pp.491~500 (2003)
- Schlemper RJ, "Differences in the diagnostic criteria used by Japanese and Western pathologists to diagnose colorectal carcinoma", Cancer, 82, pp.60~69 (1998)
- Schlemper RJ, "Review of histological classifications of gastrointestinal epithelial neoplasia: differences in diagnosis of early carcinomas between Japanese and Western pathologists", J Gastroenterol, 36, pp.445~456 (2001)
- Schlemper RJ, "Well-differentiated adenocarcinoma or dysplasia of the gastric epithelium: rationale for a new classification system", Verh Dtsch Ges Pathol, 83, pp.62~70 (1999)
- Stolte M, "Diagnosis of gastric carcinoma: Japanese fairy tales or Western deficiency?", Virchows Arch, 434, pp.279~280 (1999)
- Takenawa H, "Differential gene-expression profiles associated with gastric adenoma", Br J Cancer, 90, pp.216~223 (2004)
- Park SY, "Long-term follow-up study of gastric intraepithelial neoplasias: progression from low-grade dysplasia to invasive carcinoma", Eur J Gastroenterol Hepatol, 20, pp.966~970 (2008)
- Fearon ER, "A genetic model for colorectal tumorigenesis", Cell, 61, pp.759~767 (1990)
- Kinzler KW, "Identification of a gene located at chromosome 5q21 that is mutated in colorectal cancers", Science, 251, pp.1366~1370 (1991)
- Su LK, "Association of the APC tumor suppressor protein with catenins", Science, 262, pp.1734~1737 (1993)
- Lengauer C, "Genetic instability in colorectal cancers", Nature, 386, pp.623~627 (1997)
- Nowak MA, "The role of chromosomal instability in tumor initiation", Proc Natl Acad Sci U S A, 99, pp.16226~16231 (2002)
- Nowak MA, "Evolutionary dynamics of tumor suppressor gene inactivation", Proc Natl Acad Sci U S A, 101, pp.10635~10638 (2004)
- Shih IM, "Evidence that genetic instability occurs at an early stage of colorectal tumorigenesis", Cancer Res, 61, pp.818~822 (2001)
- Raghavan SC, "DNA structures at chromosomal translocation sites", Bioessays, 28, pp.480~494 (2006)
- Gorringe KL, "Evidence that both genetic instability and selection contribute to the accumulation of chromosome alterations in cancer", Carcinogenesis, 26, pp.923~930 (2005)
- Bayani J, "Genomic mechanisms and measurement of structural and numerical instability in cancer cells", Semin Cancer Biol, 17, pp.5~18 (2007)
- Tanaka A, "Low-grade gastric adenomas", dysplasias: phenotypic expression, DNA ploidy pattern, and LOH at microsatellites linked to the APC gene, 2008, pp.204, 1~9
- Powell SM, "APC mutations occur early during colorectal tumorigenesis", Nature, 359, pp.235~237 (1992)
- Kaplan KB, "A role for the Adenomatous Polyposis Coli protein in chromosome segregation", Nat Cell Biol, 3, pp.429~432 (2001)
- Fodde R, "Mutations in the APC tumour suppressor gene cause chromosomal instability", Nat Cell Biol, 3, pp.433~438 (2001)
- Lee JH, "Inverse relationship between APC gene mutation in gastric adenomas and development of adenocarcinoma", Am J Pathol, 161, pp.611~618 (2002)
- Ebert MP, "Increased beta-catenin mRNA levels and mutational alterations of the APC and beta-catenin gene are present in intestinal-type gastric cancer", Carcinogenesis, 23, pp.87~91 (2002)
- Nakatsuru S, "Somatic mutation of the APC gene in gastric cancer: frequent mutations in very well differentiated adenocarcinoma and signet-ring cell carcinoma", Hum Mol Genet, 1, pp.559~563 (1992)
- Fang DC, "Mutation analysis of APC gene in gastric cancer with microsatellite instability", World J Gastroenterol, 8, pp.787~791 (2002)
- Weber JL, "Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction", Am J Hum Genet, 44, pp.388~396 (1989)
- Hayden JD, "The role of microsatellite instability in gastric carcinoma", Gut, 42, pp.300~303 (1998)
- Arzimanoglou II, "Microsatellite instability in human solid tumors", Cancer, 82, pp.1808~1820 (1998)
- Weissenbach J, "A second-gen eration linkage map of the human genome", Nature, 359, pp.794~801 (1992)
- Samowitz WS, "Microsatellite instability in colorectal adenomas", Gastroenterology, 112, pp.1515~1519 (1997)
- Jernvall P, "Microsatellite instability: impact on cancer progression in proximal and distal colorectal cancers", Eur J Cancer, 35, pp.197~201 (1999)
- Young J, "Genomic instability occurs in colorectal carcinomas but not in adenomas", Hum Mutat, 2, pp.351~354 (1993)
- Garay J, "Infrequency of microsatellite instability in complete and incomplete gastric intestinal metaplasia", Hum Pathol, 35, pp.102~106 (2004)
- Leung WK, "Microsatellite instability in gastric intestinal metaplasia in patients with and without gastric cancer", Am J Pathol, 156, pp.537~543 (2000)
- Machado AM, "Helicobacter pylori infection induces genetic instability of nuclear and mitochondrial DNA in gastric cells", Clin Cancer Res, 15, pp.2995~3002 (2009)
- Mirzaee V, "Helicobacter pylori infection and expression of DNA mismatch repair proteins", World J Gastroenterol, 14, pp.6717~6721 (2008)
- Park DI, "Risk factors suggesting malignant transformation of gastric adenoma: univariate and multivariate analysis", Endoscopy, 33, pp.501~506 (2001)
- Tabata H, "Difference in degree of mucosal atrophy between elevated and depressed types of gastric epithelial tumors", Scand J Gastroenterol, 36, pp.1134~1140 (2001)
- Vieth M, "Elevated risk for gastric adenocarcinoma can be predicted from histomorphology", World J Gastroenterol, 12, pp.6109~6114 (2006)
