三黃洗劑가 Raw 264.7 cell 에서의 항염 효과에 미치는 영향
The Effects of Samhwangseje on Anti-Inflammation in Raw 264.7 cell
2
조회
0
다운로드
5
인용
0
참고문헌
키워드
초록
Objectives : This study was carried out to investigate the effects of Samhwangseje(SHSJ) on anti-Inflammation in Raw 264.7 cell. Methods : The effects of SHSJ on anti-Inflammation were measured by the cytotoxicity of Raw 264.7 cell, the inhibition for NO, TNF-⍺, PGE2, iNOS and COX-2, the blocking NF-κB into nucleus. Results : 1. All concentrations of SHSJ had no cytotoxicity in Raw 264.7 cell. 2. All concentrations of SHSJ inhibited the production of NO in the Raw 264.7 cell stimulated with LPS. 3. All concentrations of SHSJ did not inhibit the production of TNF-⍺ in the Raw 264.7 cell stimulated with LPS. 4. All concentrations of SHSJ inhibited the production of PGE2 in the Raw 264.7 cell stimulated with LPS. 5. All concentrations of SHSJ did not inhibit the expression of COX-2 but concentrations of 50 ㎍/㎖, 100 ㎍/㎖ SHSJ inhibited iNOS expression in the Raw 264.7 cell stimulated with LPS. 6. Concentrations of 50 ㎍/㎖, 100 ㎍/㎖ SHSJ had the effect of blocking NF-κB into nucleus in LPS-induced macrophage Raw 264.7 cell.
Abstract
Objectives : This study was carried out to investigate the effects of Samhwangseje(SHSJ) on anti-Inflammation in Raw 264.7 cell. Methods : The effects of SHSJ on anti-Inflammation were measured by the cytotoxicity of Raw 264.7 cell, the inhibition for NO, TNF-⍺, PGE2, iNOS and COX-2, the blocking NF-κB into nucleus. Results : 1. All concentrations of SHSJ had no cytotoxicity in Raw 264.7 cell. 2. All concentrations of SHSJ inhibited the production of NO in the Raw 264.7 cell stimulated with LPS. 3. All concentrations of SHSJ did not inhibit the production of TNF-⍺ in the Raw 264.7 cell stimulated with LPS. 4. All concentrations of SHSJ inhibited the production of PGE2 in the Raw 264.7 cell stimulated with LPS. 5. All concentrations of SHSJ did not inhibit the expression of COX-2 but concentrations of 50 ㎍/㎖, 100 ㎍/㎖ SHSJ inhibited iNOS expression in the Raw 264.7 cell stimulated with LPS. 6. Concentrations of 50 ㎍/㎖, 100 ㎍/㎖ SHSJ had the effect of blocking NF-κB into nucleus in LPS-induced macrophage Raw 264.7 cell.
참고문헌 (31)
- 顧伯茸, "實用中醫外科學", pp.535~536 (1985), 上海科學技術出版社
- 江克明, "방제대사전", pp.68~ (1991), 의성당
- 楊思澍, "중의임상대전", pp.864~ (1993), 의성당
- 葛華, "三黃洗劑治療陰部濕疹 48例", 陜西中醫, 23(3), pp.257~ (2002)
- 龔明, "三黃洗劑爲主治療汗疱疹 85例", 陜西中醫, 21(3), pp.129~ (2000)
- 張宏亮, "自擬三黃洗劑加減治療手指外傷感染 36例", 內蒙古中醫藥, 19, pp.12~ (2000)
- 董新亮, "三黃洗劑治療濕瘡 54例", 長春中醫學院學報, 15(3), pp.43~ (1999)
- 曹玉忠, "三黃洗劑治療姙娠性痒疹療效觀察", 中國現代醫藥雜志, 10(2), pp.106~ (2008)
- 石秀蘭, "中藥三黃洗劑在皮膚科的應用", 新中醫, pp.105~ (1996)
- 李舜華, "三黃洗劑在皮膚病中的運用", 江西中醫藥, pp.113~ (1996)
- 黃春黎, "蜂膠三黃洗劑治療痤瘡24例臨床觀察", 養蜂科技, pp.39~ (2004)
- 원영호, "三黃洗劑加味方의 항균, 항염 및 항알레르기효과에 대한 실험적 연구", 대한안이비인후피부과학회지, 18(1), pp.135~153 (2005)
- H. Kim, "Studies on the anti-acne effect of Agrimonia pilosa Ledeb", J. Soc. Cosmet. Scientists Korea, 32(1), pp.53~58 (2006)
- T. Mosmann, "Rapid colorimetric assay for cellular growth and survival application of proliferation and cytotoxicity", J. Immunol. Methods, 65, pp.55~ (1983)
- 한상미, "국내산 동양종 꿀벌의 벌독에 의한 RAW 264.7 세포주에서 Nitric Oxide와 Tumor Necrocis Factor-a에 미치는 항염증 작용에 관한 연구", 한국양봉학회지, 19(2), pp.89~ (2004), 한국양봉학회
- 이은방, "Studies on the antiinflammatory effects of natural products", 응용약물학회지, 6(3), pp.269~275 (1998)
- Wu MJ, "Glossogyne tenuifolia acts to inhibit inflammatory mediator production in a macrophage cell line by downregulating LPS-induced NF-κB", J Biomed Sci, 11, pp.186~199 (2004)
- 임사비나, "고량강의 항염증 작용에 대한 연구", 대한본초학회지, 20(2), pp.43~53 (2005), 대한본초학회
- 최승만, "Propionibacterium acnes에 대한 천연물의 항균효과 검색", 대한약학회지, 42, pp.89~94 (1998)
- 홍원식, "精校黃帝內經靈樞", pp.345~350 (1985), 동양의학연구원출판부
- 신연상, "유백피의 항염 및 조직재생에 대한 실험적 연구", 대한외관과학회지, 14(1), pp.129~153 (2001)
- 전국한의과대학 본초학교수, "本草學", pp.178~179 (1998), 영림사
- Han M, "Acetylbritannilatone suppresses NO and PGE2 synthesis in RAW 264.7 macrophages through the inhibition of iNOS and COX-2 gene expression", Life science, 75, pp.675~684 (2004)
- 이연아, "황련 추출물의 항염 효과", 경희의학, 23(1), pp.21~ (2007)
- Tsai, P, "Biochem", Pharmacol, 69(6), pp.971~979 (2005)
- Vilcek J, "Tumor necrosis factor; New insights into the molecular mechanism of its multiple actions", J. Biol Chem, 266, pp.7313~7316 (1991)
- 노민수, "리포폴리사카라이드에 의해 유도되는 대식세포의 프로스타글린딘 생합성을 저해하는 천연물의 탐색", 약학회지, 42, pp.558~566 (1998)
- Keller, B, "Pharmaceut Composit", Therap, 16 (2002)
- Lawrence T, "Possible new role for NF-kappa B in the resolution of inflammation", Nat Med, 7(12), pp.1291~1297 (2001)
- Barnes PJ, "NF-kappa B: a pivotal role in asthma and a new targen for therapy", Trends pharmacol. Sci, 18(2), pp.46~50 (1997)
- 김영일, "牧丹皮․桃仁 배합이 항염증 작용에 미치는 영향", 박사, pp.2005