口瘡의 外用藥으로 多用되는 黃柏, 靑黛, 白礬 및 五倍子의 抗炎作用에 관한 硏究

Abstract

This experimental study was performed to investigate the anti-inflammatory effects of Phellodendri Cortex(PC), Indigo Naturalis(IN), Alumen(AM), and Chinensis Galla(CG). These four prescriptions most frequently have been used in oriental medicine for the external theraphy of Gu-Chang. This study was done to evaluate the activity of superoxide dismutase(SOD) and the inhibitory effects of the formation of cyclooxygenase-2(COX-2), tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), prostaglandin-E2(PGE2) on the monocyte and neutrophil. The results were summerized as follows. 1. IN has no cytotoxicity but PC, AM, CG have a little cytotoxicity by the increase of concentrations. 2. PC, IN, AM and CG all inhibited the formation of COX-2 in the human neutrophil. 3. 1000㎍/㎖ of PC increased the formation of SOD in the human monocyte. 4. 1000㎍/㎖ of PC, 100㎍/㎖ and 1000㎍/㎖ of IN, 100㎍/㎖ of CG inhibited the formation of TNF-α in the human monocyte. 5. 10㎍/㎖ of PC, 1000㎍/㎖ of IN inhibited the formation of IL-1 β in the human monocyte. 6. PC, IN, AM and CG all did not inhibit the production of PGE2 in the human monocyte In addition, the results show that PC, IN, AM and CG all have anti-inflammatory effects and can be used for the external theraphy of Gu-Chang.