木香 에탄올추출물의 抗菌效果
Antibacterial Activity of Ethanol Extract of Saussurea lappa
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Objective : The purpose of this study is to investigate the antibacterial activity of Saussurea lappa for the applications of herb-based extracts to both cosmetic and medicinal industries. Methods : Antibacterial activity of ethanol extract of Saussurea lappa (ESL) was assessed using agar diffusion and broth dilution methods, and determined by whether clear zone was formed around paper disc and in terms of the size (mm) of clear zone. Results : ESL provided activity against Staphylococcus aureus KCTC 1927 in concentration of 1 ㎎/㎖ with an clear zone of 16 mm, and showed an activity against Staphylococcus epidermids KCTC 1917 in concentration of 1 ㎎/㎖ with an clear zone of 18㎜. Moreover, the minimum inhibitory concentration (MIC) of ESL against S. aureus and S. epidermidis were 1 ㎎/㎖ for both bacterial species. However, ESL showed no growth inhibition against Pseudomonas aeruginosa KCTC 12513, Enterobacter aerogenes KCTC 2190, Escherichia coli KCTC 2571, Salmonella typhimurium KCTC 1925 and Propionibacterium acnes KCCM 41747. Conclusions : Antibacterial activity of ESL against Staphylococcus aureus and Staphylococcus epidermids causing eye and skin diseases was proved. The result suggest that ESL may be useful as a natural preservative on behalf of synthetic preservatives.
Abstract
Objective : The purpose of this study is to investigate the antibacterial activity of Saussurea lappa for the applications of herb-based extracts to both cosmetic and medicinal industries. Methods : Antibacterial activity of ethanol extract of Saussurea lappa (ESL) was assessed using agar diffusion and broth dilution methods, and determined by whether clear zone was formed around paper disc and in terms of the size (mm) of clear zone. Results : ESL provided activity against Staphylococcus aureus KCTC 1927 in concentration of 1 ㎎/㎖ with an clear zone of 16 mm, and showed an activity against Staphylococcus epidermids KCTC 1917 in concentration of 1 ㎎/㎖ with an clear zone of 18㎜. Moreover, the minimum inhibitory concentration (MIC) of ESL against S. aureus and S. epidermidis were 1 ㎎/㎖ for both bacterial species. However, ESL showed no growth inhibition against Pseudomonas aeruginosa KCTC 12513, Enterobacter aerogenes KCTC 2190, Escherichia coli KCTC 2571, Salmonella typhimurium KCTC 1925 and Propionibacterium acnes KCCM 41747. Conclusions : Antibacterial activity of ESL against Staphylococcus aureus and Staphylococcus epidermids causing eye and skin diseases was proved. The result suggest that ESL may be useful as a natural preservative on behalf of synthetic preservatives.
참고문헌 (24)
- Forbes JM, "Oxidative stress as a major culprit in kidney disease in diabetes", Diabetes, 57(6), pp.1446~1454 (2008)
- 오영삼, "Photorefractive Keratectomy (PRK) 후 착용한 치료용 콘택트렌즈의 오염", 대한안과학회지, 44(2), pp.309~314 (2003), 대한안과학회
- Novotna G, "In vitro activity of telithromycin and quinupri-stin", dalfopristin against methicillin-resistant coagulase-negative staphylococci with defined resistance genotypes, 2007, pp.52(6), 593~599
- Hanssen AM, "Multiple staphylococcal cassette chromosomes and allelic variants of cassette chromosome recombinases in Staphylococcus aureus and coagulase-negative staphylococci from Norway", Antimicrob Agents Ch, 51(5), pp.1671~1677 (2007)
- Kang SH, "Isolation and quantitative determination of costunolide from saussurea root", Kor J Pharmacogn, 30, pp.48~53 (1999)
- Wedge DE, "Fungicidal activity of natural and synthetic sesquiterpene lactone analogs", Phytochemistry, 53(7), pp.747~757 (2000)
- Park HJ, "Syringin 4-O-beta-glucoside, a new phenylpropanoid glycoside, and costunolide, a nitric oxide synthase inhibitor, from the stem bark of Magnolia sieboldii", J Nat Prod, 59(12), pp.1128~1130 (1996)
- Jeong SJ, "Costunolide, a sesquiterpene lactone from Saussurea lappa, inhibits the VEGFR KDR", Flk-1 signaling pathway, 2002, pp.187(1), 129~133
- Yasunaka K, "Antibacterial activity of crued extracts from mexican medicinal plants and purified coumarins and xanthones", J Ethnopharmacol, 97(2), pp.293~299 (2005)
- Jin-Woo Lee, "Bacterial Communities in the Initial Stage of Marine Biofilm Formation on Artificial Surfaces", The Journal of Microbiology, 46(2), pp.174~182 (2008), 한국미생물학회
- Little AE, "Black yeast symbionts compromise the efficiency of antibiotic defenses in fungus-growing ants", Ecology, 89(5), pp.1216~1222 (2008)
- Paladino JA, "Influence of extended-spectrum beta-lactams on gramnegative bacterial resistance", Am J Health Syst Pharm, 65(12), pp.1154~1159 (2008)
- Bastos EM, "In vitro study of the antivicrobial activity of Brazilian propolis against Paenibacillus larvae", J Invest Pathol, 97(3), pp.273~281 (2008)
- Olgen S, "Antimicrobial and antiviral screening of novel indole carboxamide and propanamide derivatives", Z Naturforsch, 63(3), pp.189~195 (2008)
- Hu J, "Phenotyping and genotyping of antibiotic-resistant Escherichia coli isolated from a natural river basin", Environ Sci Technol, 42(9), pp.3415~3420 (2008)
- 신태양사 편집국 백과사전부, "원색최신의료대백과사전", pp.47~48 (1994), 신태양사
- 윤동호, "안과학", pp.2~3 (1996), 일조각
- 장영식, "Staphylococcus epidermidis 각막염의 역학조사", 대한안과학회지, 43(4), pp.4~671 (2002), 대한안과학회
- 정희영, "감염질환", pp.665~675 (1987), 수문사
- 相賀徹夫, "中藥大辭典", pp.2528~ (1985), 上海科學技術出版社
- Ko SG, "Induction of apoptosis by Saussurea lappa and Pharbitis nil on AGS gastric cancer cells", Biol Pharm Bull, 27(10), pp.1604~1610 (2004)
- Ko SG, "Saussurea lappa induces G2-growth arrest and apoptosis in AGS gastric cancer cells", Cancer Lett, 220(1), pp.11~19 (2005)
- Mori H, "Chemopreventive effect of costunolide, a constituent of oriental medicine, on azoxymethane-induced intestinal carcinogenesis in rats", Cancer Lett, 83(1), pp.171~175 (1994)
- Chen CN, "Isocostunolide, a sesquiterpene lactone, induces mitochondrial membrane depolarization and caspase-dependent apoptosis in human melanoma cells", Cancer Lett, 246(1), pp.237~252 (2007)