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PPAR-γ조절을 통한 麗澤通氣湯의 항알레르기와 항염증 효과

The Antiallergic Effects of Yeotaectonggi-tang (YTT) on the Mice Model with Allergic Rhinitis by Regulation of PPAR-γ

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Objectives : The aim of this study is to investigate the antiallergic effects of YTT on allergic rhinitis by regulation of peroxisome proliferator-activated receptor gamma (PPAR-γ). Methods : The thirty rats were divided into three groups : normal group, control (allergic rhinitis elicited)group, sample (Yeotaectonggi-tang treated after allergic rhinitis elicitation) group. To induce allergic rhinitis in control group and sample group, rats were sensitized intraperitoneally with 0.1% ovalbumin(OVA)solution 3 times at intervals of 1 week. Then intranasal sensitization was performed by diffusing 0.1%ovalbumin(OVA) solution 3 times at intervals of 2 days. After that time rats in sample group were oral administration treated by YTT for 7days. The change of the amounts of eosinophil, substance P, MIP-2,PPAR-γ, IL-4, iNOS were observed in each group. we used the statistical method of ANOVA test(p<0.05). Results : The number of eosinophil in sample group noticeably decreased than control group. And the decrease of substance P and MIP-2 positive reaction were observed in mucosa. YTT inhibited IL-4 and iNOS production, mucus secretion, activation of mast cells and fibrosis remodeling by regulation of PPAR-γactivation. Conclusion : According to the above results, it is considered that YTT mitigated mucosa damage on the mice model with allergic rhinitis by regulation of PPAR-γ.

Abstract

Objectives : The aim of this study is to investigate the antiallergic effects of YTT on allergic rhinitis by regulation of peroxisome proliferator-activated receptor gamma (PPAR-γ). Methods : The thirty rats were divided into three groups : normal group, control (allergic rhinitis elicited)group, sample (Yeotaectonggi-tang treated after allergic rhinitis elicitation) group. To induce allergic rhinitis in control group and sample group, rats were sensitized intraperitoneally with 0.1% ovalbumin(OVA)solution 3 times at intervals of 1 week. Then intranasal sensitization was performed by diffusing 0.1%ovalbumin(OVA) solution 3 times at intervals of 2 days. After that time rats in sample group were oral administration treated by YTT for 7days. The change of the amounts of eosinophil, substance P, MIP-2,PPAR-γ, IL-4, iNOS were observed in each group. we used the statistical method of ANOVA test(p<0.05). Results : The number of eosinophil in sample group noticeably decreased than control group. And the decrease of substance P and MIP-2 positive reaction were observed in mucosa. YTT inhibited IL-4 and iNOS production, mucus secretion, activation of mast cells and fibrosis remodeling by regulation of PPAR-γactivation. Conclusion : According to the above results, it is considered that YTT mitigated mucosa damage on the mice model with allergic rhinitis by regulation of PPAR-γ.

참고문헌 (68)

  1. Divya Seth, "Elizabeth Secord, and Deepak Kamat. Allergic Rhinitis", Clinical Pediatrics,, 46, pp.401~407 (2007)
  2. Strachan D, "Worldwide variations in prevalence of symptoms of allergic rhinoconjunctivitis in children:the international Study of Asthma and Allergies in Childhood (ISAAC)", Pediatr Allergy Immunol, 8(4), pp.161~176 (1997)
  3. 지혜미, "2006년 초등학생의 알레르기 질환 유병률의 전국적 역학조사", 대한소아알레르기및호흡기학회지, 19(2), pp.165~172 (2009), 대한 소아알레르기 호흡기학회
  4. 白萬基, "最新耳鼻咽喉科學", pp.169~172 (2001), 一潮閣
  5. 李東垣, "東垣十種醫書", pp.179~180 (1993), 一中社
  6. 許俊, "對譯東醫寶鑑", pp.592~ (1999), 法仁文化社
  7. 손동석, "비질환의 치료방제에 대한 문헌적 고찰", 대한외관과학회지, 14(1), pp.11~31 (2001)
  8. 윤찬호, "비색의 병인.병기.치료에 대한 문헌적 고찰", 대한외관과학회지, 12(1), pp.295~312 (1999)
  9. 박진구, "여택통기탕과 여택통기탕가미방의 항알레르기 효과에 대한 실험적 연구", 동의학회지, 5(1), pp.76~97 (2001)
  10. 고정순, "仙麗澤通氣湯이 ovalbumin에 의하여 유발된 allergic mouse의 면역기능에 미치는 影響", 한방안이비인후피부과학회지, 18(1), pp.94~103 (2005), 대한한방안이비인후피부과학회
  11. Jiang C, "PPAR-gamma agonists inhibit production of monocyte inflammatory cytokines", Nature, 391(6662), pp.82~86 (1998)
  12. Jeon EJ, "The effects of peroxisome proliferator-activated receptor-gamma agonist on a murine model of experimental allergic rhinitis", Otolaryngol Head Neck Surg, 139(1), pp.124~130 (2008)
  13. 대한천식및알레르기학회, "천식과 알레르기 질환", pp.189~ (2002), 군자출판사
  14. 蔡炳允, "韓方眼耳鼻咽喉科學", pp.321~326 (1982), 集文堂
  15. 盧石善, "原色眼耳卑咽喉科學", pp.558~561 (2003), 周珉出版社
  16. Rosenwasser L, "New insights into the pathophysiology of allergic rhinitis", Allergy Asthma Proc, 28, pp.10~15 (2007)
  17. KleinJan A, "Basophil and eosinophil accumulation and mast cell degranulation in the nasal mucosa of pathents with hay fever after local allergen provocation", J Allergy Clin Immunol, 106, pp.677~686 (2000)
  18. Christodoulopoulos P, "Molecular pathology of allergic disease Ⅱ : Upper airway disease", J Allergy Clin Immunol., 105, pp.211~223 (2000)
  19. 이주희, "비색증과 알레르기비염에 관한 문헌적 고찰(원인과 증상을 중심으로)", 대한한방내과학회지, 14(1), pp.26~34 (1993)
  20. 曹孝忠, "聖濟總錄 下", pp.948~ (1978), 新文豊出版公司
  21. 襲廷賢, "萬病回春 下", pp.14~, 행림서원
  22. 서부일, "龍葵의 免疫調節作用 및 알레르기 低減化에 關한 硏究", 대한한의학방제학회, 5(1), pp.184~202 (1997)
  23. Du Q, "Inhibitory effects of astragaloside IV on ovalbumin-induced chronic experimental asthma", Can J Physiol Pharmacol, 86(7), pp.449~457 (2008)
  24. Cho JH, "Inhibitory effects of diterpene acids from root of Aralia cordata on IgE-mediated asthma in guinea pigs", Pulm Pharmacol Ther, 23(3), pp.190~199 (2010)
  25. 최수형, "수종 생약의 항알레르기 작용", 약학회지, 36(2), pp.140~149 (1992)
  26. 곽상교, "감초가 천식모델 생쥐의 혈청내 Cytokine에 미치는 효과", 동의생리병리학회지, 18(2), pp.463~467 (2004), 대한동의병리학회
  27. 송상진, "감초가 천식모델 생쥐의 plasma내 histamine과 폐조직내 cytokien 생성에 미치는 효과", 동의생리병리학회지, 18(3), pp.841~845 (2004), 대한동의병리학회
  28. 한영주, "감초(Glycyrrhiza uralensis Fisch, GLU)가 천식모델 생쥐의 BALF내 면역세포 및 Cytokine에 미치는 효과", 대한한방내과학회지, 25(3), pp.408~417 (2004), 대한한방내과학회
  29. Liu YG, "Effects of among compositions of Herba Ephedraedecoction on genic xpression of 5-lipoxygenase activating protein, IL-4 and leukotriene C4 in asthmatic mice", Zhongguo Zhong Yao Za Zhi, 32(3), pp.246~249 (2007)
  30. Lee J, "Inhibitory effect of phytoglycoprotein (24kDa) on allergyrelated factors in compound 48", 80-induced mast cells in vivo and in vitro, 2010, pp.10(5), 591~599
  31. Ahui ML, "Ginger prevents Th2-mediated immune responses in a mouse model of airway inflammation", Int Immunopharmacol, 8(12), pp.1626~1632 (2008)
  32. Ryu M, "Astragali Radix elicits anti-inflammation via activation of MKP-1, concomitant with attenuation of p38 and Erk", J Ethnopharmacol, 115(2), pp.184~193 (2008)
  33. Jang MH, "Atractylodes japonica suppresses lipopolysaccharide-stimulated expressions of inducible nitric oxide synthase and cyclooxygenase-2 in RAW 264.7 macrophages", Biol Pharm Bull, 27(3), pp.324~327 (2004)
  34. 이도연, "강활과 방풍의 항염증 상승작용에 관한 연구", 대한본초학회지, 23(4), pp.39~44 (2008), 대한본초학회
  35. Hyun Lim, "Anti-inflammatory Activity of the Constituents of the Roots of Aralia continentalis", Archives of Pharmacal Research, 32(9), pp.1237~1243 (2009), 대한약학회
  36. Sakai S, "Inhibitory effect of ferulic acid and isoferulic acid on the production of macrophage inflammatory protein-2 in response to respiratory syncytial virus infection in RAW264.7 cells", Mediators Inflamm, 8(3), pp.173~175 (1999)
  37. Hirabayashi T, "Inhibitory effect of ferulic acid and isoferulic acid on murine interleukin-8 production in response to influenza virus infections in vitro and in vivo", Planta Med, 61(3), pp.221~226 (1995)
  38. 김영란, "승마의 항알러지작용 및 항염증작용:활성성분의 부분적 정제", 한국응용약물학회지, 2(2), pp.149~154 (1994)
  39. Kim IT, "Anti-inflammatory and anti-nociceptive effects of the extract from Kalopanax pictus, Pueraria thunbergiana and Rhus verniciflua", J Ethnopharmacol, 94(1), pp.165~173 (2004)
  40. Shin EM, "Anti-inflammatory effects of glycyrol isolated from Glycyrrhizauralensis in LPS-stimulated RAW264.7 macrophages", Int Immunopharmacol, 8(11), pp.1524~1532 (2008)
  41. Kim JY, "Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-kappaB in RAW 264.7 macrophages", Eur J Pharmacol, 584(1), pp.175~184 (2008)
  42. Lee SJ, "Inhibitory effect of ZPDC glycoprotein on the expression of inflammation-related cytokines through p38 MAP kinase and JNK in lipopolysaccharide-stimulated RAW 264.7 cells", Inflamm Res, 58(4), pp.184~191 (2009)
  43. Lee SJ, "ZPDC glycoprotein inhibits inflammation-related cytokine and protein via nuclear factor-kappa B in dextran sulfate sodium-stimulated ICR mouse", Biomed Pharmacother, 63(7), pp.528~536 (2009)
  44. 이상현, "芷貝散의 抗炎症 效能에 대한 硏究", 대한한의학 방제학회지, 16(1), pp.79~94 (2008), 대한한의학방제학회
  45. Lin CH, "Byakangelicol, isolated from Angelica dahurica, inhibits both the activity and induction of cyclooxygenase-2 in human pulmonary epithelial cells", J Pharm Pharmacol, 54(9), pp.1271~1278 (2002)
  46. 정환용, "腦神經小膠細胞에서 생강 헥산 분획물의 염증매개물질 生成 抑制效果", 대한한의학회지, 30(2), pp.17~29 (2009), 대한한의학회
  47. -shogaol, "J Ethnopharmacol", 2010, 515~520
  48. Ahui ML, "Ginger prevents Th2-mediated immune responses in a mouse model of airway inflammation", Int Immunopharmacol, 8(12), pp.1626~ (2008)
  49. 김희택, "후각장애 환자에서의 麗澤通氣湯 치험 1례", 동의생리병리학회지, 15(5), pp.830~832 (2001), 대한동의병리학회
  50. Woerly G, "Peroxisome proliferatoractivated receptors alpha and gamma down-regulate allergic inflammation andeosinophil activation", J Exp Med, 198(3), pp.411~421 (2003)
  51. Won HY, "Anti-allergic function and regulatory mechanisms of KR62980 in allergen-induced airway inflammation", Biochem Pharmacol, 79(6), pp.888~896 (2010)
  52. Hammad H, "Activation of peroxisome proliferator-activated receptor-gamma in dendritic cells inhibits the development of eosinophilic airway inflammation in a mouse model of asthma", Am J Pathol, 164(1), pp.263~271 (2004)
  53. Koreck AI, "Rhinophototherapy: a new therapeutic tool for the management of allergic rhinitis", J Allergy Clin Immunol, 115(3), pp.541~547 (2005)
  54. Rousset F, "Shifts in interleukin-4 and interferon-gamma production by T cells of patients with elevated serum IgE levels and the modulatory effects of these lymphokines on spontaneous IgE synthesis", J Allergy Clin Immunol, 87(1), pp.58~69 (1991)
  55. Trifilieff A, "PPAR-alpha and -gamma but not -delta agonists inhibit airway inflammation in a murine model of asthma: in vitro evidence for an NF-kappaB-independent effect", Br J Pharmacol, 139(1), pp.163~171 (2003)
  56. Hibbs JB Jr, "Macrophage cytotoxicity: role for L-arginine deiminase and imino nitrogen oxidation to nitrite", Science, 235(4787), pp.473~476 (1987)
  57. Toda N, "Nitric oxide:ocular blood flow, glaucoma, and diabetic retinopathy", Prog Retin Eye Res, 26(3), pp.205~238 (2007)
  58. Aktan F, "iNOS-mediated nitric oxide production and its regulation", Life Sci, 75(6), pp.639~653 (2004)
  59. Radi R, "Peroxynitrite-induced membrane lipid peroxidation: the cytotoxic potential of superoxide and nitric oxide", Arch Biochem Biophys, 288(2), pp.481~487 (1991)
  60. Villa L. M, "Peroxynitrite induces both vasodilatation and impaired vascular relaxation in the isolated perfused rat heart", Proc Natl Acad nSci USA, 91(26), pp.12383~12387 (1994)
  61. Robbins RA, "Grisham MB. Nitric oxide", Int J Biochem Cell Biol, 29, pp.857~904 (1997)
  62. Honda K, "Peroxisome proliferatoractivated receptor gamma is expressed in airways and inhibits features of airway remodeling in a mouse asthma model", J Allergy Clin Immunol, 113(5), pp.882~888 (2004)
  63. Sugiyama H, "Peroxisome proliferator-activated receptors are expressed in human cultured mast cells: a possible role of these receptors in negative regulation of mast cell activation", Eur J Immunol, 30(12), pp.3363~3370 (2000)
  64. Pfaar O, "Pathophysiology of itching and sneezing in allergic rhinitis", 139(3), pp.35~40 (2009)
  65. Misery L, "Skin, immunity, and nervous system", Br J Dermatol, 137, pp.843~859 (1997)
  66. Sherry, "Resolution of the two components of macrophage inflammatory protein 1, and cloning and characterization of one of those components, macrophage inflammatory protein 1-beta", J. Exp. Med, 168, pp.2251~2259 (1988)
  67. Irving, "Two inflammatory mediator cytokine genes are closely linked and variably amplified on chromosome 17q", Nucleic Acids Res., 18, pp.3261~3270 (1990)
  68. Yoshida S, "Involvement of IL-8, vascular endothelial growth factor, and basic fibroblast growth factor in TNF-α dependent angiogenesis", Mol. Cell Biol, 17, pp.4015~4023 (1997)

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