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환경강화가 뇌손상 흰쥐의 해마에서 BDNF 발현과 기능변화에 미치는 영향

Effects of Enrichmented Environment on the Expression of BDNF in the Hippocampus and Functional Change of Rats Whose Brain was Damaged

황보 각|임창훈
특수교육재활과학연구소 (The Research Institute for Special Education & Rehabilitation Science)|2008.06|특수교육재활과학연구|제47권 제2호|pp. 223-240

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키워드

뇌가소성brain plasticity환경강화신경영양성 물질environmental enrichmentneuro-trophic factor
주제분류기타사회과학

초록

뇌손상 질환의 장애를 가진 환자들의 치료에는 다양한 방법들이 사용되어지고 있다. 뇌손상의 회복과 관련하여 현재에는 기능적인 측면뿐만 아니라 뇌신경의 형태학적 구조가 재생된 뇌가소성을 말하고 있다. 그래서 강화된 환경이 뇌의 가소성과 관련이 있는 신경영양물질 중 BDNF 발현에 영향을 미치는지 규명하며, 기능적 변화에도 영향을 주는지 확인하여 다양한 뇌질환 환자들의 치료방법 중 가정에서 강화된 환경의 일상생활을 통해 기능적 회복과 시간, 경제적 측면에서 효과적이며 간편한 치료방법을 재활 전문가들에게 제안하고자 하였다.

Abstract

This study aims to find out whether or not neuro-trophic factors are expressed in rats whose brain was damaged by the evironmental enrichment. This study was analyzed with experimental animals. For this study, mature male Sprague-Dawley family rats, which are 7-9 weeks old, 250-300g in weight, healthy and having no trouble neurologically, were randomly selected and had an operation under anesthesia to remove the somatomotor area of rata. After conducting an operation of rats, the rats were randomly divided into two groups to raise them: 3 rats of the experimental group to be raised in the environment-reinforced cage, and 3 rats of the control group to be raised in the standard cage. BNDF expression was inspected in the hippocampus of rats with immunohistochemistry on 1, 3, 7, 14 days after operation. As a result of the inspection, BDNF expression and dendrite increased in the hippocampus of rats in the environment- reinforced cage, BDNF was synthesized for recovery of damage, and, as the time passes by, the number increased. And the effect is presumed to be continuous for regeneration due to changes of morphological changes of cranial nerves. neurological behavior tests(Postural Reflex Test, Beam-Walking Test) were showed that the experimental group which underwent evironmental enrichment cage had the highest neurological behavior test score except for the day 1 group. On the basis of such results, it is considered that reinforced environment leads to an increase of the expression of neuro-trophic factors, thus advances performance and becomes a part of various mechanisms which disclose plasticity of the central nervous system. In conclusion, environment enrichment is an effective treatment for patients of brain injury.

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