3 차원 co-culture 시스템을 통한 BMSC의 NP-like cell로의 분화
The Differentiation of bone Marrow Stromal Cells into NP-like Cells through 3-Dimensional Co-culture System
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The goal of this study is to investigate the effect and potential of three-dimensional Co-culture of BMSCs (bone marrow stromal Cells) and NP (nucleus pulposus) Cells on the differentiation of BMSCs into NP-like Cells.The NP Cells and BMSCs were isolated and cultured from New Zealand White rabbits. The isolated NP Cells and BMSCs were prepared in different alginate beads. Those two types of beads were separated by a track-etched membrane of 3 μm pore in a 6-well culture plate. No growth factors were used. In addition to these, NP and BMSC were cultured in the beads independently for control. The number of Cells in Co-culturing system was half of those in two control groups. Proliferation and production of glycosaminoglycan (GAG) were evaluated along with histological observation. The GAG production rate(GAG contents/Cell) of Co-cultured BMSCs were much higher than that of BMSCs cultured alone. The total amounts of GAG produced by BMSCs in Co-culturing system were larger than those produced by BMSCs in control group and were comparable with those produced by NP alone even the number of each Cell was half of BMSCs in Co-culturing system.This study showed the potential of differentiation of BMSCs into NP-like Cells through three-dimensional Co-culture system even without any chemical agents.
Abstract
The goal of this study is to investigate the effect and potential of three-dimensional Co-culture of BMSCs (bone marrow stromal Cells) and NP (nucleus pulposus) Cells on the differentiation of BMSCs into NP-like Cells.The NP Cells and BMSCs were isolated and cultured from New Zealand White rabbits. The isolated NP Cells and BMSCs were prepared in different alginate beads. Those two types of beads were separated by a track-etched membrane of 3 μm pore in a 6-well culture plate. No growth factors were used. In addition to these, NP and BMSC were cultured in the beads independently for control. The number of Cells in Co-culturing system was half of those in two control groups. Proliferation and production of glycosaminoglycan (GAG) were evaluated along with histological observation. The GAG production rate(GAG contents/Cell) of Co-cultured BMSCs were much higher than that of BMSCs cultured alone. The total amounts of GAG produced by BMSCs in Co-culturing system were larger than those produced by BMSCs in control group and were comparable with those produced by NP alone even the number of each Cell was half of BMSCs in Co-culturing system.This study showed the potential of differentiation of BMSCs into NP-like Cells through three-dimensional Co-culture system even without any chemical agents.
참고문헌 (16)
- Butler D, "Disc degenerate before facets", Spine, 15, pp.111~113 (1990)
- Lipson SJ, "Proteoglycans in experimental intervertebral disc degeneration", Spine, 6, pp.194~210 (1981)
- Gruber HE, "Autologous intervertebral disc Cell implantation: a model using Psammomys obesus, the sand rat", Spine, 27, pp.1626~1633 (2002)
- Mizuno H, "Tissue-engineered comp- osites of anulus fibrosus and nucleus pulposus for intervertebral disc replacement", Spine, 29, pp.1290~1297 (2004)
- Maroudas A, "Factors invol- ved in the nutrition of the human lumber intervertebral disc: Cellularity and diffusion of glucose in vitro", J Anat, 120, pp.113~130 (1975)
- Stephen M, "Intervertebral Disc Cell-Mediated Mesencymal Stem Cell Differentiation", Stem cells, 24, pp.707~716 (2006)
- Steck E, "Induction of Intervertebral Disc-Like Cells From Adult Mesenchymal Stem Cells", Stem cells, 23, pp.403~411 (2005)
- Risbud MV, "Differentiation of mesenchymal stem Cells towards a nucleus pulposus-like phenotype in vitro: Implications for Cell-based transplantation therapy", Spine, 29, pp.2627~2632 (2004)
- Aguiar DJ, "Notochordal Cells Interact with Nucleus Pulposus Cells: Regulation of Proteoglycan Synthesis", Exp Cell Res, 246, pp.129~137 (1999)
- Yamamoto Y, "Upregulation of the Viability of Nucleus Pulposus Cells by Bone Marrow-Derived Stromal Cells. Significance of Direct Cell-to-Cell Contact in Coculture System", Spine, 29, pp.1508~1514 (2004)
- Aulthous AL, "Expression of the human chondrocyte phenotype in vitro", In Vitro Cell Dev Biol, 25, pp.659~668 (1989)
- Archer CW, "Phenotypic modulation in sub-populations of human articular chondrocytes in vitro", J Cell Sci, 97, pp.361~371 (1990)
- Kimura T, "Chondrocytes embedded in collagen gels maintain cartilage phenotype during long-term cultures", Clin Orthop, 186, pp.231~239 (1984)
- Bassleer C, "Human chondrocytes in tridimensional culture", In Vitro Cell Dev Biol, 22, pp.113~119 (1986)
- Beekman B, "Synthesis of collagen by bovine chondrocytes cultured in alginate; posttranslational modifications and Cell-matrix interaction", Exp Cell Res, 237, pp.135~141 (1997)
- Richardson SM, "The differentiation of bone marrow mesenchymal stem Cells into chondrocyte-like Cells on poly-L-lactic acid (PLLA) scaffolds", Biomaterials, 27, pp.4069~4078 (2006)