3차원 다공성 콜라겐지지체의 제조 및 특성 분석
Fabrication and Characterization of 3-D Porous Collagen Scaffold
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Collagen scaffolds were synthesized by cross linking into a solution mixture of 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochlorid(EDC) in ethanol, followed by pressing, cleaning and lyophilization processafter the type I atelo-collagen solutions in D.I water(pH3). The experimental conditions are collagen concentrationof 1.0 wt%, 3.0 wt%, 5.0 wt% and differential concentration of cross-linker. Then, parametric studies were performedby varying the parameters to investigate the morphology, the porosity, the swelling ratio and the thickness and genotoxicityof the scaffolds. The scaffolds thickness pattern was regular to concentration of the degree of cross-linkerand collagen. It was observed that the swelling ratio, the degree of crosslink, and the pore size(thickness of scaffold)can be controlled by adjusting the collagen, crosslinker. Among the parameters investigated, the smallest thicknesscan be achieved by collagen, crosslinker concentrate condition. The collagen scaffold is induced no genotoxicity. Thelowest swelling ratio, as an indication of the highest degree of crosslink, can be obtained by adding crosslink agent.
Abstract
Collagen scaffolds were synthesized by cross linking into a solution mixture of 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochlorid(EDC) in ethanol, followed by pressing, cleaning and lyophilization processafter the type I atelo-collagen solutions in D.I water(pH3). The experimental conditions are collagen concentrationof 1.0 wt%, 3.0 wt%, 5.0 wt% and differential concentration of cross-linker. Then, parametric studies were performedby varying the parameters to investigate the morphology, the porosity, the swelling ratio and the thickness and genotoxicityof the scaffolds. The scaffolds thickness pattern was regular to concentration of the degree of cross-linkerand collagen. It was observed that the swelling ratio, the degree of crosslink, and the pore size(thickness of scaffold)can be controlled by adjusting the collagen, crosslinker. Among the parameters investigated, the smallest thicknesscan be achieved by collagen, crosslinker concentrate condition. The collagen scaffold is induced no genotoxicity. Thelowest swelling ratio, as an indication of the highest degree of crosslink, can be obtained by adding crosslink agent.
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