BioPAC 모듈을 이용한 마우스 시각유발전위 측정 시스템 확립
Measuring System of Visual Evoked Potential (VEP) in Mice using BioPAC Modules
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
For the development of feasible retinal prosthesis, one of the important elements is acquiring proper judging tool if electrical stimulus leads to patient’s visual perception. If evoked potential to electrical stimulus is recorded in primary visual (V1) cortex, it means that the stimulus effectively evokes visual perception. Therefore, in this study, we established VEP recording system on V1 cortex using BioPAC modules as the judging tool. And the measuring system was evaluated by recording VEP of mice. After anesthesia, normal mice (C57BL/6J strain; n = 6) were secured to stereotaxic apparatus (Harvard Apparatus, USA). For the recording of VEP, the stainless steel needle electrode (impedance: 2-5 kΩ) was positioned on the surface of the cortex through the burr hole at 2.5 mm lateral and 4.6 mm caudal to bregma. DA 100C and EEG 100C BioPAC modules were used for the trigger signal and VEP recording, respectively. When left eye was blocked by black cover and right eye was stimulated by flash light using HMsERG (RetVet Corp, USA), VEP response at left V1 cortex was detected, but there was no response at right V1 cortex. Amplitudes and latencies of P2, N3 peaks of VEP recording varied according to the depths of the electrodes on V1 cortex. From the surface upto 600 μm depth, amplitudes of P2 and N3 increased, while deeper than 600 μm, those amplitudes decreased. The deeper the insertion depth of the electrode, the latency of N1 peaks tends to be delayed. However, there was no statistically significant difference among the latencies of P2 and N3 peaks (P > 0.05, ANOVA). Our VEP recording data such as the insertion depth and the latency and amplitudes of peaks might be used as guidelines for electrically-evoked potential (EEP) recording experiment in near future.
Abstract
For the development of feasible retinal prosthesis, one of the important elements is acquiring proper judging tool if electrical stimulus leads to patient’s visual perception. If evoked potential to electrical stimulus is recorded in primary visual (V1) cortex, it means that the stimulus effectively evokes visual perception. Therefore, in this study, we established VEP recording system on V1 cortex using BioPAC modules as the judging tool. And the measuring system was evaluated by recording VEP of mice. After anesthesia, normal mice (C57BL/6J strain; n = 6) were secured to stereotaxic apparatus (Harvard Apparatus, USA). For the recording of VEP, the stainless steel needle electrode (impedance: 2-5 kΩ) was positioned on the surface of the cortex through the burr hole at 2.5 mm lateral and 4.6 mm caudal to bregma. DA 100C and EEG 100C BioPAC modules were used for the trigger signal and VEP recording, respectively. When left eye was blocked by black cover and right eye was stimulated by flash light using HMsERG (RetVet Corp, USA), VEP response at left V1 cortex was detected, but there was no response at right V1 cortex. Amplitudes and latencies of P2, N3 peaks of VEP recording varied according to the depths of the electrodes on V1 cortex. From the surface upto 600 μm depth, amplitudes of P2 and N3 increased, while deeper than 600 μm, those amplitudes decreased. The deeper the insertion depth of the electrode, the latency of N1 peaks tends to be delayed. However, there was no statistically significant difference among the latencies of P2 and N3 peaks (P > 0.05, ANOVA). Our VEP recording data such as the insertion depth and the latency and amplitudes of peaks might be used as guidelines for electrically-evoked potential (EEP) recording experiment in near future.
참고문헌 (23)
- Schiefer U, "Clinical Neuro-Ophthalmology: A Practical Guide" (2007), Springer
- Park JY, "Visual Evoked Potentials", Clin Neuroophthalmol, 4(1), pp.27~32 (2014)
- Odom JV, "ISCEV Standard for Clinical Visual Evoked Potentials (2009 update)", Doc Ophthalmol, 120(1), pp.111~119 (2010)
- Slotnick SD, "Using multi-stimulus VEP source localization to obtain a retinotopic map of human primary visual cortex", Clin Neurophysiol, 110(10), pp.1793~1800 (1999)
- Di Russo F, "Cortical sources of the early components of the visual evoked potential", Hum Brain Mapp, 15(2), pp.95~111 (2002)
- 김성훈, "시각유발전위 검사상 후-시신경교차부위 병변을 보인 환자들의 뇌 영상 결과와의 연관성", Annals of Clinical Neurophysiology, 2(1), pp.13~20 (2000), 대한임상신경생리학회
- Sieving PA, "Ophthalmology" (2008), Elsevier
- Arnold JJ, "Age related macular degeneration", Clin Evid, 701 (2007)
- Stingl K, "Subretinal Visual Implant Alpha IMS--Clinical trial interim report", Vision Res, 111, pp.149~160 (2015)
- Luo YH, "The $Argus(^{(R)})$ II Retinal Prosthesis System", Prog Retin Eye Res, 50, pp.89~107 (2016)
- Goo YS, "Retinal ganglion cell responses to voltage and current stimulation in wild-type and rd1 mouse retinas", J Neural Eng, 8, pp.035003~ (2011)
- Ye JH, "The slow wave component of retinal activity in rd", rd mouse recorded with a multi-electrode array, 2007, pp.28, 1079~1088
- 예장희, "Functional Connectivity Map of Retinal Ganglion Cells for Retinal Prosthesis", The Korean Journal of Physiology & Pharmacology, 12(6), pp.307~314 (2008), 대한약리학회
- 구용숙, "Spontaneous Oscillatory Rhythm in Retinal Activities of Two Retinal Degeneration (rd1 and rd10) Mice", The Korean Journal of Physiology & Pharmacology, 15(6), pp.415~422 (2011), 대한약리학회
- 재설아, "Electrophysiological and Histologic Evaluation of the Time Course of Retinal Degeneration in the rd10 Mouse Model of Retinitis Pigmentosa", The Korean Journal of Physiology & Pharmacology, 17(3), pp.229~235 (2013), 대한약리학회
- Martin M, "Myelin Deficiencies Visualized In Vivo: VEP and T2-Weighted MRI of Shiverer Mutant and Wild-Type mice", J Neurosci Res, 84(8), pp.1716~1726 (2006)
- Ridder WH, "The Visual Evoked Potential in the Mouse-Origins and Response Characteristics", Vision Res, 46, pp.902~913 (2006)
- Scott-McKean JJ, "The Mouse Model of Down Syndrome Ts65Dn Presents Visual Deficits as Assessed by Pattern Visual Evoked Potential", Invest Ophthalmol Vis Sci, 51(6), pp.3300~3308 (2010)
- Paxinos G, "The mouse brain in stereotaxic coordinates" (2004), Elsevier Academic Press
- Leamey CA, "Ten_m3 Regulates Eye-Specific Patterning in the Mammalian Visual Pathway and Is Required for Binocular Vision", PLoS Biol, 5(9), pp.2077~2092 (2007)
- Bear MF, "Neuroscience: Exploring the Brain" (2007), Lippincott Williams & Wilkins
- Porciatti V, "The Visual Physiology of the Wild Type Mouse Determined with Pattern VEPs", Vision Res, 39(18), pp.3071~3081 (1999)