저출력 레이져 자극이 근육세포의 증식 및 유전자 발현에 미치는 효과
Effect of Low-Energy Laser Irradiation on the Proliferation and Gene Expression of Myoblast Cells
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Laser irradiation is known to affect various tissues such as skin, bone, nerve, and skeletal muscle. Laser irradiation promotes ATP synthesis, facilitates wound healing, and stimulates cell proliferation and angiogenesis. In skeletal muscle, laser irradiation is related to the proliferation of skeletal muscle satellite cells. Normal skeletal muscle contains remodeling capacity from myogenic cells that are derived from mononuclear satellite cells. Their processes are activated by the expression of genes related with myogenesis such as muscle-specific transcription factors (MyoD and Myf5) and VEGF (vascular endothelial growth factor). In this study, we hypothesized that laser irradiation would enhance and regulate muscle cell proliferation and regeneration through modulation of the gene expressions related with the differentiation of skeletal muscle satellite cells. C2C12 myoblastic cells were exposed to continuous/non-continuous laser irradiation (660nm/808nm) for 10 minutes daily for either 1 day or 5 days. After laser irradiation, cell proliferation and gene expression (MyoD, Myf5, VEGF) were quantified. Continuous 660nm laser irradiation significantly increased cell proliferation and gene expression compared to control, continuous 808nm laser irradiation, and non-continuous 660nm laser irradiation groups. These results indicate that continuous 660nm laser irradiation can be applied to the treatment and regeneration of skeletal muscle tissue.
Abstract
Laser irradiation is known to affect various tissues such as skin, bone, nerve, and skeletal muscle. Laser irradiation promotes ATP synthesis, facilitates wound healing, and stimulates cell proliferation and angiogenesis. In skeletal muscle, laser irradiation is related to the proliferation of skeletal muscle satellite cells. Normal skeletal muscle contains remodeling capacity from myogenic cells that are derived from mononuclear satellite cells. Their processes are activated by the expression of genes related with myogenesis such as muscle-specific transcription factors (MyoD and Myf5) and VEGF (vascular endothelial growth factor). In this study, we hypothesized that laser irradiation would enhance and regulate muscle cell proliferation and regeneration through modulation of the gene expressions related with the differentiation of skeletal muscle satellite cells. C2C12 myoblastic cells were exposed to continuous/non-continuous laser irradiation (660nm/808nm) for 10 minutes daily for either 1 day or 5 days. After laser irradiation, cell proliferation and gene expression (MyoD, Myf5, VEGF) were quantified. Continuous 660nm laser irradiation significantly increased cell proliferation and gene expression compared to control, continuous 808nm laser irradiation, and non-continuous 660nm laser irradiation groups. These results indicate that continuous 660nm laser irradiation can be applied to the treatment and regeneration of skeletal muscle tissue.
참고문헌 (13)
- Mauro A, "Satellite cell of skeletal muscle fibers", J Biophys Biochem Cytol, 9, pp.493~495 (1961)
- Peng H, "Muscle-derived stem cells for musculoskeletal tissue regeneration and repair", Transpl Immunol, 12(3), pp.311~319 (2004)
- Holterman CE, "Molecular regulation of satellite cell function", Semin Cell Dev Biol, 16(4), pp.575~584 (2005)
- Martin P, "Growth factors and cutaneous wound repair", Prog Growth Factor Res, 4(1), pp.25~44 (1992)
- Nemeth GG, "Growth factors and their role in wound and fracture healing", Prog Clin Biol Res, 266, pp.1~17 (1988)
- Conlan MJ, "Biostimulation of wound healing by low-energy laser irradiation", A review. J Clin Periodontol, 23(5), pp.492~496 (1996)
- Yaakobi T, "Promotion of bone repair in the cortical bone of the tibia in rats by low energy laser (He-Ne) irradiation", Calcif Tissue Int, 59(4), pp.297~300 (1996)
- Assia E, "Temporal parameters of low energy laser irradiation for optimal delay of post-traumatic degeneration of rat optic nerve", Brain Res, 476(2), pp.205~212 (1989)
- Bibikova A, "Promotion of muscle regeneration in the toad (Bufo viridis) gastrocnemius muscle by low-energy laser irradiation", Anat Rec, 235(3), pp.374~380 (1993)
- Karu T, "Primary and secondary mechanisms of action of visible to near-IR radiation on cells", J Photochem Photobiol B, 49(1), pp.1~17 (1999)
- MoRimoto Y, "Effect of low-intensity argon laser irradiation on mitochondrial respiration", Lasers Surg Med, 15(2), pp.191~199 (1994)
- Yu W, "Photomodulation of oxidative metabolism and electron chain enzymes in rat liver mitochondria", Photochem Photobiol, 66(6), pp.866~871 (1997)
- Weiss N, "Enhancement of muscle regeneration in the rat gastrocnemius muscle by low energy laser irradiation", Anat Embryol (Berl), 186(5), pp.497~503 (1992)