국내 Acinetobacter 임상분리주 내 adeG, adeB, adeE, adeY, abeM 및 adeJ 유출 펌프 유전자의 분포
Distribution of adeG, adeB, adeE, adeY, abeM, and adeJ Efflux Pump Genes in Clinical Isolates of Acinetobacter Species from Korea
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
배경본 연구의 목적은 국내의 병원들에서 수집된 Acinetobacter 임상분리주가 보유한 6가지 유출 펌프 유전자의 빈도를 측정하는데 있다. 방법본 연구에서 사용한 총 339주의 Acinetobacter 균주는 Acinetobacter calcoaceticus–Acinetobacter baumannii (ACB) complex 279주와 non-ACB complex Acinetobacter 60주로 구성되었다. 다약제 유출 시스템인 AdeFGH와 AdeABC, AdeDE, AdeXYZ, AbeM 및 AdeIJK 유출 펌프 각각의 운송(transporter) 유전자인 adeG와 adeB, adeE, adeY, abeM 및 adeJ 유전자를 검출하기 위해 각 유출 펌프 유전자에 특이적인 PCR 분석을 수행하였다. 결과adeG와 adeB, adeE, adeY, abeM 및 adeJ의 6가지 유출 펌프 유전자의 빈도는 14군의 Acinetobacter 균종별로 다양하게 나타났다. A. baumannii 군과 A. nosocomialis 군에서 adeE와 abeM, adeJ의 양성률은 두 균종 간에 유의한 차이가 있었다. A. baumannii 154주는 SAB, MDRAB, XDRAB 군별로 adeB와 adeE, adeY 및 adeJ의 빈도에 유의한 차이를 보였다. Imipenem-감수성군과 imipenem-비감수성군은 adeB와 adeY, adeJ의 양성률에 유의한 차이를 보였다. Ciprofloxacin-감수성군과 ciprofloxacin-비감수성군은 adeB와 adeY의 양성률에 유의한 차이를 보였다. 2012년에 수집한 86주의 A. baumannii는 수집지역별로 6가지 유출 펌프 유전자 모두 양성률에 유의한 차이가 없었다. 결론이 연구에서 얻어진 6가지 유출 펌프 유전자의 양성률은 국내 Acinetobacter 임상분리주 내 유출 펌프 유전자의 기본적인 역학적 특성을 보여주었다.
Abstract
BackgroundThe aim of the present study was to determine the frequency of six efflux pump genes in Acinetobacter clinical isolates collected from South Korean hospitals. MethodsIn this study, we used a total of 339 Acinetobacter strains, comprising 279 Acinetobacter calcoaceticus–Acinetobacter baumannii (ACB) complex and 60 non-ACB complex strains. We performed specific PCR assays to detect adeG, adeB, adeE, adeY, abeM, and adeJ, transporter genes of the multidrug efflux pumps AdeFGH, AdeABC, AdeDE, AdeXYZ, AbeM, and AdeIJK, respectively. ResultsFrequencies of six efflux pump genes varied according to the species of Acinetobacter . Frequencies of adeE, abeM, and adeJ between A. baumannii group and A. nosocomialis group were found to be significantly different. Significant differences were found in the frequencies of adeB, adeE, adeY, and adeJ among the susceptible A. baumannii (SAB), multidrug-resistant A. baumannii (MDRAB), and extensively drug-resistant A. baumannii (XDRAB) groups within the 154 strains of A. baumannii. The frequencies of efflux pump genes in imipenem-susceptible and imipenemnonsusceptible groups were significantly different for adeB, adeY, and adeJ. The frequencies of efflux pump genes in ciprofloxacin-susceptible and ciprofloxacin-nonsusceptible groups were significantly different for adeB and adeY. No significant difference was found in the frequency of efflux pump genes among groups sampled from different regions of Korea, across 86 strains of A. baumannii collected in 2012. ConclusionsThe frequencies of six efflux pump genes obtained in this study demonstrate the fundamental epidemiological feature of efflux pump genes in Korean Acinetobacter clinical isolates.
참고문헌 (23)
- Coyne S, "efflux-mediated antibiotic resistance in Acinetobacter spp", Antimicrob Agents Chemother, 55, pp.947~953 (2011)
- Hernando-Amado S, "Multidrug efflux pumps as main players in intrinsic and acquired resistance to antimicrobials", Drug Resist Updat, 28, pp.13~27 (2016)
- Yoon EJ, "Contribution of resistance-nodulation-cell division efflux systems to antibiotic resistance and bio lm formation in Acinetobacter baumannii", MBio, 6, pp.e00309~15 (2015)
- Magiorakos AP, "Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria : an international expert proposal for interim standard de nitions for acquired resistance", Clin Microbiol Infect, 18, pp.268~281 (2012)
- Higgins PG, "A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU", Clin Microbiol Infect, 13, pp.1199~1201 (2007)
- Higgins PG, "gyrB multiplex PCR to differentiate between Acinetobacter calcoaceticus and Acinetobacter genomic species 3", J Clin Microbiol, 48, pp.4592~4594 (2010)
- La Scola B, "Sequencing of the rpoB gene and flanking spacers for molecular identi cation of Acinetobacter species", J Clin Microbiol, 44, pp.827~832 (2006)
- Woodford N, "Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp", Int J Antimicrob Agents, 27, pp.351~353 (2006)
- Lee MJ, "Comparison of rpoB gene sequencing, 16S rRNA gene sequencing, gyrB multiplex PCR, and the VITEK2 system for identification of Acinetobacter clinical isolates", Diagn Microbiol Infect Dis, 78, pp.29~34 (2014)
- Li XM, "Development and evaluation of species-specific PCR for detection of nine Acinetobacter species", Ann Clin Lab Sci, 46, pp.270~278 (2016)
- Coyne S, "Overexpression of resistance-nodulation-cell division pump AdeFGH confers multidrug resistance in Acinetobacter baumannii", Antimicrob Agents Chemother, 54, pp.4389~4393 (2010)
- Magnet S, "Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454", Antimicrob Agents Chemother, 45, pp.3375~3380 (2001)
- Chau SL, "Novel resistance-nodulation-cell division efflux system AdeDE in Acinetobacter genomic DNA group 3", Antimicrob Agents Chemother, 48, pp.4054~4055 (2004)
- Chu YW, "Presence of active efflux systems AdeABC, AdeDE and AdeXYZ in different Acinetobacter genomic DNA groups", J Med Microbiol, 55, pp.477~478 (2006)
- Su XZ, "AbeM, an H+-coupled Acinetobacter baumannii multidrug efflux pump belonging to the MATE family of transporters", Antimicrob Agents Chemother, 49, pp.4362~4364 (2005)
- Damier-Piolle L, "AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii", Antimicrob Agents Chemother, 52, pp.557~562 (2008)
- Lin L, "Distribution of the multidrug efflux pump genes, adeABC, adeDE and adeIJK, and class 1 integron genes in multiple-antimicrobial-resistant clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus complex", Int J Antimicrob Agents, 33, pp.27~32 (2009)
- Nowak J, "Prevalence of eight resistance-nodulation-division efflux pump genes in epidemiologically characterized Acinetobacter baumannii of worldwide origin", J Med Microbiol, 64, pp.630~635 (2015)
- Wieczorek P, "Distribution of AdeABC efflux system genes in genotypically diverse strains of clinical Acinetobacter baumannii", Diagn Microbiol Infect Dis, 77, pp.106~109 (2013)
- Nemec A, "Relationship between the AdeABC efflux system gene content, netilmicin susceptibility and multidrug resistance in a genotypically diverse collection of Acinetobacter baumannii strains", J Antimicrob Chemother, 60, pp.483~489 (2007)
- Huys G, "Distribution of tetracycline resistance genes in genotypically related and unrelated multiresistant Acinetobacter baumannii strains from different European hospitals", Res Microbiol, 156, pp.348~355 (2005)
- Pagdepanichkit S, "Distribution and expression of the Ade multidrug efflux systems in Acinetobacter baumannii clinical isolates", Can J Microbiol, 62, pp.794~801 (2016)
- Hou PF, "Study of the correlation of imipenem resistance with efflux pumps AdeABC, AdeIJK, AdeDE and AbeM in clinical isolates of Acinetobacter baumannii", Chemotherapy, 58, pp.152~158 (2012)