혈청단백 모세관전기영동의 기술적 고려 사항과 전기영동도의 다양한 특징
Technical Considerations and Miscellaneous Findings on Electrophoretograms of Serum Protein Capillary Electrophoresis
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
혈청단백 모세관전기영동은 겔 기반의 검사법보다 자동화가 용이하여 임상검사실에서 많이 사용되고 있다. 전통적으로 많이 사용되어 온 아가로스겔전기영동과 비교하여 모세관전기영동은 그 검사 원리와 기술적 특성이 다르므로 나타나는 검사상의 문제와 전기영동도의 특성이 차이가 있으며, 이를 이해하는 것은 검사상의 다양한 문제 해결과 결과의 검증에 도움이 된다. 또한 전기영동도에서 단클론면역글로불린 단백 이외의 원인에 의한 다양한 이상피크를 보일 수 있으므로, 이들의 특성을 이해하고 이상 결과에 대해 적절히 대응하여야 한다. 혈청단백 모세관전기영동 검사의 정확한 판독을 위해서는 적절한 교육을 통한 검사 원리의 이해와 다양한 사례에 대한 경험이 판독자에게 필요할 것이다.
Abstract
The use of the serum protein capillary electrophoresis (CE) is prevalent in clinical laboratories owing to its greater feasibility for automation compared with the gel-based methods. Compared with the traditionally commonly used agarose gel electrophoresis, CE exhibits distinctive test principle and technical characteristics, thereby showing different test problems and electrophoretogram characteristics. Proficient comprehension of these properties facilitates the resolution of diverse test problems and verification of results. Electrophoretogram of CE may manifest diverse abnormal peaks beyond monoclonal immunoglobulin protein. Interpreter should understand their characteristics and respond appropriately to abnormal results. To accurately interpret the serum protein CE test, the interpreter would be required to understand the test method and gain experience with various cases through appropriate training.
참고문헌 (55)
- Keren DF, "Protein electrophoresis in clinical diagnosis" (2012), American Society for Clinical Pathology Press
- Jacobs JFM, "An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part II : limit of detection and follow-up of patients with small M-proteins", Clin Chem Lab Med, 58, pp.547~559 (2020)
- Moss MA, "Moving towards harmonized reporting of serum and urine protein electrophoresis", Clin Chem Lab Med, 54, pp.973~979 (2016)
- Chan PC, "On the path to evidence-based reporting of serum protein electrophoresis patterns in the absence of a discernible monoclonal protein-A critical review of literature and practice suggestions", Clin Biochem, 51, pp.29~37 (2018)
- Bazydio LAL, "Tietz textbook of laboratory medicine", pp.225~240 (2023), Elsevier
- Hage DS, "An Overview of CE in clinical analysis", Methods Mol Biol, 1972, pp.3~11 (2019)
- Bossuyt X, "Advances in serum protein electrophoresis", Adv Clin Chem, 42, pp.43~80 (2006)
- Chen FT, "Capillary electrophoresis—a new clinical tool", Clin Chem, 37, pp.14~19 (1991)
- Genzen JR, "Screening and diagnosis of monoclonal gammopathies : an international survey of laboratory practice", Arch Pathol Lab Med, 142, pp.507~515 (2018)
- 조주영, "국내 29개 병원의 혈청단백 전기영동 및 면역고정 전기영동 검사현황 고찰", Laboratory Medicine Online, 12(2), pp.91~99 (2022), 대한진단검사의학회[DOI]
- Regeniter A, "Peaks and tails : Evaluation of irregularities in capillary serum protein electrophoresis", Clin Biochem, 51, pp.48~55 (2018)
- Chartier C, "Evaluation of two automated capillary electrophoresis systems for human serum protein analysis", Clin Biochem, 44, pp.1473~1479 (2011)
- Wijnen PA, "Capillary electrophoresis of serum proteins. Reproducibility, comparison with agarose gel electrophoresis and a review of the literature", Eur J Clin Chem Clin Biochem, 34, pp.535~545 (1996)
- Gay-Bellile C, "Automated multicapillary electrophoresis for analysis of human serum proteins", Clin Chem, 49, pp.1909~1915 (2003)
- Tsui AKY, "Now you see it, now you don’t : a case of covert(invisible)IgM", Clin Biochem, 51, pp.101~102 (2018)
- Clark R, "Differential diagnosis of gammopathies by capillary electrophoresis and immunosubtraction : analysis of serum samples problematic by agarose gel electrophoresis", Electrophoresis, 19, pp.2479~2484 (1998)
- Keren DF, "2-Mercaptoethanol treatment improves measurement of an IgMkappa M-protein by capillary electrophoresis", Clin Chem, 47, pp.1326~1327 (2001)
- Zetterberg H, "False-negative result in the detection of an IgM monoclonal protein by capillary zone electrophoresis", Clin Chem, 50, pp.1878~1880 (2004)
- Schild C, "Monoclonal gammopathy missed by capillary zone electrophoresis", Clin Chem Lab Med, 49, pp.1217~1219 (2011)
- Jenkins MA, "Optimization of serum protein separation by capillary electrophoresis", Clin Chem, 42, pp.1886~ (1996)
- Brouwers A, "Interference of ceftriaxone on capillary zone electrophoresis", Clin Chim Acta, 376, pp.255~256 (2007)
- Kobayashi S, "Bisalbumin(fast and slow type)induced by human pancreatic juice", Ann Clin Biochem, 32, pp.63~67 (1995)
- 고대현, "Identification of Compound Heterozygous Mutation in a Korean Patient with Alpha 1-antitrypsin Deficiency", Annals of Laboratory Medicine, 31(4), pp.294~297 (2011), 대한진단검사의학회
- Kyle RA, "Review of 1027 patients with newly diagnosed multiple myeloma", Mayo Clin Proc, 78, pp.21~33 (2003)
- Kirk RL, "Transferrin variants in Korea and Japan", Jinrui Idengaku Zasshi, 23, pp.1~7 (1978)
- Gijbels K, "Interference by gelatin-based plasma substitutes in capillary zone electrophoresis", Clin Chem, 50, pp.1473~1475 (2004)
- Bossuyt X, "Interferences in clinical capillary zone electrophoresis of serum proteins", Electrophoresis, 25, pp.1485~1487 (2004)
- Bossuyt X, "Automated serum protein electrophoresis by Capillarys", Clin Chem Lab Med, 41, pp.704~710 (2003)
- Chan PC, "Value of reflex testing based on hypogammaglobulinemia as demonstrated in serum protein electrophoresis", Clin Biochem, 48, pp.674~678 (2015)
- Lakshminarayanan R, "Detection by immunofixation of M proteins in hypogammaglobulinemic patients with normal serum protein electrophoresis results", Am J Clin Pathol, 127, pp.746~751 (2007)
- McCudden CR, "Recognition and management of common, rare, and novel serum protein electrophoresis and immunofixation interferences", Clin Biochem, 51, pp.72~79 (2018)
- Arranz-Peña ML, "Interference of iodinated contrast media in serum capillary zone electrophoresis", Clin Chem, 46, pp.736~737 (2000)
- Wheeler RD, "Large abnormal peak on capillary zone electrophoresis due to contrast agent", Ann Clin Biochem, 55, pp.608~611 (2018)
- Mills JR, "Identification of friend or foe : the laboratory challenge of differentiating M-proteins from monoclonal antibody therapies", J Appl Lab Med, 1, pp.421~431 (2017)
- McCudden C, "Monitoring multiple myeloma patients treated with daratumumab : teasing out monoclonal antibody interference", Clin Chem Lab Med, 54, pp.1095~1104 (2016)
- Noori S, "Monitoring the M-protein of multiple myeloma patients treated with a combination of monoclonal antibodies : the laboratory solution to eliminate interference", Clin Chem Lab Med, 59, pp.1963~1971 (2021)
- Liu L, "A novel approach to remove interference of therapeutic monoclonal antibody with serum protein electrophoresis", Clin Biochem, 75, pp.40~47 (2020)
- Thoren KL, "Distinguishing drug from disease by use of the Hydrashift 2", 4 daratumumab assay, 2019, pp.3, 857~863
- Huls F, "Expression of daratumumab and elotuzumab migration by capillary electrophoresis relative to transferrin improves precision of their identification", J Appl Lab Med, 5, pp.419~422 (2020)
- Katzmann JA, "Prospective study of serum protein capillary zone electrophoresis and immunotyping of monoclonal proteins by immunosubtraction", Am J Clin Pathol, 110, pp.503~509 (1998)
- Bossuyt X, "Detection and classification of paraproteins by capillary immunofixation", subtraction, 1998, pp.44, 760~764
- Howard BM, "A comparison of gel(Hydragel 30)and capillary(Capillarys III Tera)electrophoresis for the characterization of human serum proteins", Pract Lab Med, 25, pp.e00233~ (2021)
- Poisson J, "Performance evaluation of the Helena V8 capillary electrophoresis system", Clin Biochem, 45, pp.697~699 (2012)
- Huang RS, "High false-positive rate for monoclonal gammopathy using capillary electrophoresis(CAPILLARYS 2)alone", J Clin Lab Anal, 28, pp.42~46 (2014)
- Lissoir B, "Serum protein electrophoresis : comparison of capillary zone electrophoresis Capillarys(Sebia)and agarose gel electrophoresis Hydrasys(Sebia)", Ann Biol Clin(Paris), 61, pp.557~562 (2003)
- McCudden CR, "Performance comparison of capillary and agarose gel electrophoresis for the identification and characterization of monoclonal immunoglobulins", Am J Clin Pathol, 129, pp.451~458 (2008)
- Keren DF, "Laboratory detection and initial diagnosis of monoclonal gammopathies", Arch Pathol Lab Med, 146, pp.575~590 (2022)
- Rajkumar SV, "International Myeloma Working Group updated criteria for the diagnosis of multiple myeloma", Lancet Oncol, 15, pp.e538~e548 (2014)
- Kumar S, "International Myeloma Working Group consensus criteria for response and minimal residual disease assessment in multiple myeloma", Lancet Oncol, 17, pp.e328~e346 (2016)
- Turner KA, "An international multi-center serum protein electrophoresis accuracy and M-protein isotyping study. Part I : factors impacting limit of quantitation of serum protein electrophoresis", Clin Chem Lab Med, 58, pp.533~546 (2020)
- Tate J, "Recommendations for standardized reporting of protein electrophoresis in Australia and New Zealand", Ann Clin Biochem, 49, pp.242~256 (2012)
- Tate JR, "A global call to arms for clinical laboratories – Harmonised quantification and reporting of monoclonal proteins", Clin Biochem, 51, pp.4~9 (2018)
- Yang Z, "Performance of the Sebia CAPILLARYS 2 for detection and immunotyping of serum monoclonal paraproteins", Am J Clin Pathol, 128, pp.293~299 (2007)
- Thoren KL, "Immunotyping provides equivalent results to immunofixation in a population with a high prevalence of monoclonal gammopathies", J Appl Lab Med, 6, pp.1551~1560 (2021)
- Miyazaki K, "Capillary electrophoresis", immunosubtraction as a better alternative to immunofixation for detecting and immunotyping serum monoclonal proteins in patients with immunoglobulin light chain(AL)amyloidosis, 2016, pp.23, 221~224
